Initial cell type divergence in Dictyostelium is independent of DIF-1 - PubMed (original) (raw)
. 1996 Mar 15;174(2):214-20.
doi: 10.1006/dbio.1996.0067.
Affiliations
- PMID: 8631494
- DOI: 10.1006/dbio.1996.0067
Free article
Initial cell type divergence in Dictyostelium is independent of DIF-1
G Shaulsky et al. Dev Biol. 1996.
Free article
Abstract
Prespore and prestalk cells can be distinguished within aggregates of Dictyostelium by the expression of well-characterized cell type-specific genes. Fusion of the tagB regulatory region to Escherichia coli beta-galactosidase revealed that this prestalk specific gene marks the differentiation of the initial prestalk cell population, PST-1. The reporter gene was expressed normally in tagB- mutant cells despite the fact that they do not accumulate measurable levels of DIF-I, a morphogen that was previously implicated in prestalk differentiation. In an independent experimental system, wild-type cells respond to the addition of DIF-I by induction of the prestalk marker ecmA and repression of the prespore marker cotB. We found that DIF-1 did not affect the expression of the tagB or carB genes, both of which are prestalk specific and essential for PST-A cell differentiation. We conclude that the initiation of prestalk development is not dependent on DIF-1 and suggest that the morphogen participates mainly at later stages.
Similar articles
- A multidrug resistance transporter/serine protease gene is required for prestalk specialization in Dictyostelium.
Shaulsky G, Kuspa A, Loomis WF. Shaulsky G, et al. Genes Dev. 1995 May 1;9(9):1111-22. doi: 10.1101/gad.9.9.1111. Genes Dev. 1995. PMID: 7744252 - The cAMP receptor subtype cAR2 is restricted to a subset of prestalk cells during Dictyostelium development and displays unexpected DIF-1 responsiveness.
Saxe CL 3rd, Yu Y, Jones C, Bauman A, Haynes C. Saxe CL 3rd, et al. Dev Biol. 1996 Mar 15;174(2):202-13. doi: 10.1006/dbio.1996.0066. Dev Biol. 1996. PMID: 8631493 - Autonomous and nonautonomous regulation of axis formation by antagonistic signaling via 7-span cAMP receptors and GSK3 in Dictyostelium.
Ginsburg GT, Kimmel AR. Ginsburg GT, et al. Genes Dev. 1997 Aug 15;11(16):2112-23. doi: 10.1101/gad.11.16.2112. Genes Dev. 1997. PMID: 9284050 Free PMC article. - [Transcription factors and cell differentiation: roles of STAT proteins in Dictyostelium].
Kawata T. Kawata T. Tanpakushitsu Kakusan Koso. 1998 Jun;43(7):825-33. Tanpakushitsu Kakusan Koso. 1998. PMID: 9635388 Review. Japanese. No abstract available. - Signalling pathways that direct prestalk and stalk cell differentiation in Dictyostelium.
Early A. Early A. Semin Cell Dev Biol. 1999 Dec;10(6):587-95. doi: 10.1006/scdb.1999.0342. Semin Cell Dev Biol. 1999. PMID: 10706823 Review.
Cited by
- SDF-2 induction of terminal differentiation in Dictyostelium discoideum is mediated by the membrane-spanning sensor kinase DhkA.
Wang N, Söderbom F, Anjard C, Shaulsky G, Loomis WF. Wang N, et al. Mol Cell Biol. 1999 Jul;19(7):4750-6. doi: 10.1128/MCB.19.7.4750. Mol Cell Biol. 1999. PMID: 10373524 Free PMC article. - Cheating by exploitation of developmental prestalk patterning in Dictyostelium discoideum.
Khare A, Shaulsky G. Khare A, et al. PLoS Genet. 2010 Feb 26;6(2):e1000854. doi: 10.1371/journal.pgen.1000854. PLoS Genet. 2010. PMID: 20195510 Free PMC article. - Dictyostelium amoebae lacking an F-box protein form spores rather than stalk in chimeras with wild type.
Ennis HL, Dao DN, Pukatzki SU, Kessin RH. Ennis HL, et al. Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3292-7. doi: 10.1073/pnas.97.7.3292. Proc Natl Acad Sci U S A. 2000. PMID: 10725352 Free PMC article. - Expression of an activated rasD gene changes cell fate decisions during Dictyostelium development.
Louis SA, Spiegelman GB, Weeks G. Louis SA, et al. Mol Biol Cell. 1997 Feb;8(2):303-12. doi: 10.1091/mbc.8.2.303. Mol Biol Cell. 1997. PMID: 9190209 Free PMC article. - Control of cell type proportioning in Dictyostelium discoideum by differentiation-inducing factor as determined by in situ hybridization.
Maruo T, Sakamoto H, Iranfar N, Fuller D, Morio T, Urushihara H, Tanaka Y, Maeda M, Loomis WF. Maruo T, et al. Eukaryot Cell. 2004 Oct;3(5):1241-8. doi: 10.1128/EC.3.5.1241-1248.2004. Eukaryot Cell. 2004. PMID: 15470253 Free PMC article.