Myofibroblasts differentiate from fibroblasts when plated at low density - PubMed (original) (raw)

Myofibroblasts differentiate from fibroblasts when plated at low density

S K Masur et al. Proc Natl Acad Sci U S A. 1996.

Abstract

Myofibroblasts, defined by their expression of smooth muscle alpha-actin, appear at corneal and dermal incisions and promote wound contraction. We report here that cultured fibroblasts differentiate into myofibroblasts by a cell density-dependent mechanism. Fibroblasts seeded at low density (5 cells per mm2) produced a cell culture population consisting of 70-80% myofibroblasts, 5-7 days after seeding. In contrast, fibroblasts seeded at high density (500 cells per mm2) produced cultures with only 5-10% myofibroblasts. When the myofibroblast-enriched cultures were subsequently passaged at high density, the smooth muscle alpha-actin phenotype was lost within 3 days. Furthermore, initially 60% of the low density-cultured cells incorporated BrdUrd compared to 30% of cells passaged at high density. Media from myofibroblast-enriched cultures had more latent and active transforming growth factor beta (TGF-beta) than did media from fibroblast-enriched cultures. Although there was a trend towards increased numbers of myofibroblasts after addition of exogenous TGF-beta, the results did not reach statistical significance. We conclude that myofibroblast differentiation can be induced in fibroblasts by plating at low density. We propose a cell density-dependent model of myofibroblast differentiation during wounding and healing in which at least two factors interact: loss of cell contact and the presence of TGF-beta.

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References

1. Science. 1970 Dec 4;170(3962):1095-6 - PubMed
1. Am J Ophthalmol. 1957 Oct;44(4 Pt 2):173-80; discussion 180-2 - PubMed
1. Invest Ophthalmol Vis Sci. 1980 Oct;19(10):1204-21 - PubMed
1. J Cell Biol. 1986 Feb;102(2):343-52 - PubMed
1. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4894-7 - PubMed

Myofibroblasts differentiate from fibroblasts when plated at low density - PubMed (original) (raw)