Decreased skeletal muscle phosphotyrosine phosphatase (PTPase) activity towards insulin receptors in insulin-resistant Zucker rats measured by delayed Europium fluorescence - PubMed (original) (raw)

Comparative Study

doi: 10.1007/BF00403956.

Affiliations

• PMID: 8635665
• DOI: 10.1007/BF00403956

Comparative Study

Decreased skeletal muscle phosphotyrosine phosphatase (PTPase) activity towards insulin receptors in insulin-resistant Zucker rats measured by delayed Europium fluorescence

D Worm et al. Diabetologia. 1996 Feb.

Abstract

In order to measure the phosphotyrosine phosphatase (PTPase) activity in small muscle biopsies, a sandwich-immunofluorescence assay was developed using the phosphorylated human insulin receptor as a substrate, a C-terminal insulin receptor antibody as catching antibody and Europium-labelled anti-phosphotyrosine as detecting antibody. Soluble and particulate muscle fractions were prepared from soleus muscle of obese, diabetic (fa/fa) Zucker rats and their lean littermates (Fa/-). In the soluble muscle fractions of the obese (fa/fa) rats PTPase activity was significantly reduced compared to control (Fa/-) rats (45.2 +/- 2.6% vs 61.3 +/- 4.7%, p < 0.02). This reduction was completely prevented by 24 days of metformin treatment which decreased plasma glucose and plasma insulin levels. In particulate muscle fractions, however, no difference in PTPase activity was found among any groups of rats examined. These results show that the alterations in soluble PTPase activity in the insulin-resistant, diabetic Zucker rat vary with the abnormality in glucose homeostasis.

PubMed Disclaimer

Similar articles

• Altered basal and insulin-stimulated phosphotyrosine phosphatase (PTPase) activity in skeletal muscle from NIDDM patients compared with control subjects.
  Worm D, Vinten J, Staehr P, Henriksen JE, Handberg A, Beck-Nielsen H. Worm D, et al. Diabetologia. 1996 Oct;39(10):1208-14. doi: 10.1007/BF02658508. Diabetologia. 1996. PMID: 8897009
• Effects of adenosine receptor antagonism on protein tyrosine phosphatase in rat skeletal muscle.
  Crist GH, Xu B, Berkich DA, LaNoue KF. Crist GH, et al. Int J Biochem Cell Biol. 2001 Aug;33(8):817-30. doi: 10.1016/s1357-2725(01)00051-6. Int J Biochem Cell Biol. 2001. PMID: 11404185
• Alterations in skeletal muscle protein-tyrosine phosphatase activity and expression in insulin-resistant human obesity and diabetes.
  Ahmad F, Azevedo JL, Cortright R, Dohm GL, Goldstein BJ. Ahmad F, et al. J Clin Invest. 1997 Jul 15;100(2):449-58. doi: 10.1172/JCI119552. J Clin Invest. 1997. PMID: 9218523 Free PMC article.
• Metformin-insulin interactions: from organ to cell.
  Wiernsperger N, Rapin JR. Wiernsperger N, et al. Diabetes Metab Rev. 1995 Sep;11 Suppl 1:S3-12. doi: 10.1002/dmr.5610110503. Diabetes Metab Rev. 1995. PMID: 8529482 Review. No abstract available.

Cited by

• Characterization of selective resistance to insulin signaling in the vasculature of obese Zucker (fa/fa) rats.
  Jiang ZY, Lin YW, Clemont A, Feener EP, Hein KD, Igarashi M, Yamauchi T, White MF, King GL. Jiang ZY, et al. J Clin Invest. 1999 Aug;104(4):447-57. doi: 10.1172/JCI5971. J Clin Invest. 1999. PMID: 10449437 Free PMC article.
• Altered basal and insulin-stimulated phosphotyrosine phosphatase (PTPase) activity in skeletal muscle from NIDDM patients compared with control subjects.
  Worm D, Vinten J, Staehr P, Henriksen JE, Handberg A, Beck-Nielsen H. Worm D, et al. Diabetologia. 1996 Oct;39(10):1208-14. doi: 10.1007/BF02658508. Diabetologia. 1996. PMID: 8897009

References

1. 1. J Biol Chem. 1989 Jun 5;264(16):9497-504 - PubMed
2. 1. J Clin Endocrinol Metab. 1986 Oct;63(4):898-905 - PubMed
3. 1. Diabetologia. 1987 Jun;30(6):437-40 - PubMed
4. 1. J Clin Invest. 1991 Apr;87(4):1286-94 - PubMed
5. 1. Endocrinology. 1972 May;90(5):1320-30 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program