Native 3D structure of eukaryotic 80s ribosome: morphological homology with E. coli 70S ribosome - PubMed (original) (raw)
Comparative Study
Native 3D structure of eukaryotic 80s ribosome: morphological homology with E. coli 70S ribosome
A Verschoor et al. J Cell Biol. 1996 May.
Abstract
A three-dimensional reconstruction of the eukaryotic 80S monosome from a frozen-hydrated electron microscopic preparation reveals the native structure of this macromolecular complex. The new structure, at 38A resolution, shows a marked resemblance to the structure determined for the E. coli 70S ribosome (Frank, J., A. Verschoor, Y. Li, J. Zhu, R.K. Lata, M. Radermacher, P. Penczek, R. Grassucci, R.K. Agrawal, and Srivastava. 1996b. In press; Frank, J., J. Zhu, P. Penczek, Y. Li, S. Srivastava ., A. Verschoor, M. Radermacher, R. Grassucci, R.K. Lata, and R. Agrawal. 1995. Nature (Lond.).376:441-444.) limited to a comparable resolution, but with a number of eukaryotic elaborations superimposed. Although considerably greater size and intricacy of the features is seen in the morphology of the large subunit (60S vs 50S), the most striking differences are in the small subunit morphology (40S vs 30S): the extended beak and crest features of the head, the back lobes, and the feet. However, the structure underlying these extra features appears to be remarkably similar in form to the 30S portion of the 70S structure. The intersubunit space also appears to be strongly conserved, as might be expected from the degree of functional conservation of the ribosome among kingdoms (Eukarya, Eubacteria, and Archaea). The internal organization of the 80S structure appears as an armature or core of high-density material for each subunit, with the two cores linked by a single bridge between the platform region of the 40S subunit and the region below the presumed peptidyltransferase center of the 60S subunit. This may be equated with a close contact of the 18S and 28S rRNAs in the translational domain centered on the upper subunit:subunit interface.
Similar articles
- Three-dimensional structure of the yeast ribosome.
Verschoor A, Warner JR, Srivastava S, Grassucci RA, Frank J. Verschoor A, et al. Nucleic Acids Res. 1998 Jan 15;26(2):655-61. doi: 10.1093/nar/26.2.655. Nucleic Acids Res. 1998. PMID: 9421530 Free PMC article. - A map of protein-rRNA distribution in the 70 S Escherichia coli ribosome.
Svergun DI, Nierhaus KH. Svergun DI, et al. J Biol Chem. 2000 May 12;275(19):14432-9. doi: 10.1074/jbc.275.19.14432. J Biol Chem. 2000. PMID: 10799526 - Three-dimensional electron cryomicroscopy of ribosomes.
Stark H. Stark H. Curr Protein Pept Sci. 2002 Feb;3(1):79-91. doi: 10.2174/1389203023380873. Curr Protein Pept Sci. 2002. PMID: 12370013 Review. - Structural studies of the translational apparatus.
Agrawal RK, Frank J. Agrawal RK, et al. Curr Opin Struct Biol. 1999 Apr;9(2):215-21. doi: 10.1016/S0959-440X(99)80031-1. Curr Opin Struct Biol. 1999. PMID: 10322215 Review.
Cited by
- Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation.
Titlow J, Robertson F, Järvelin A, Ish-Horowicz D, Smith C, Gratton E, Davis I. Titlow J, et al. J Cell Biol. 2020 Mar 2;219(3):e201903135. doi: 10.1083/jcb.201903135. J Cell Biol. 2020. PMID: 32040548 Free PMC article. - Joachim Frank's Binding with the Ribosome.
Li W, Agrawal RK. Li W, et al. Structure. 2019 Mar 5;27(3):411-419. doi: 10.1016/j.str.2018.11.006. Epub 2018 Dec 27. Structure. 2019. PMID: 30595455 Free PMC article. Review. - Setting Up Shop: The Formation and Function of the Viral Factories of Cauliflower mosaic virus.
Schoelz JE, Leisner S. Schoelz JE, et al. Front Plant Sci. 2017 Oct 30;8:1832. doi: 10.3389/fpls.2017.01832. eCollection 2017. Front Plant Sci. 2017. PMID: 29163571 Free PMC article. Review. - Structure of the 40S ribosomal subunit of Plasmodium falciparum by homology and de novo modeling.
Mwangi HN, Wagacha P, Mathenge P, Sijenyi F, Mulaa F. Mwangi HN, et al. Acta Pharm Sin B. 2017 Jan;7(1):97-105. doi: 10.1016/j.apsb.2016.10.003. Epub 2016 Dec 7. Acta Pharm Sin B. 2017. PMID: 28119814 Free PMC article. - Advances in the characterization of RNA-binding proteins.
Marchese D, de Groot NS, Lorenzo Gotor N, Livi CM, Tartaglia GG. Marchese D, et al. Wiley Interdiscip Rev RNA. 2016 Nov;7(6):793-810. doi: 10.1002/wrna.1378. Epub 2016 Aug 8. Wiley Interdiscip Rev RNA. 2016. PMID: 27503141 Free PMC article. Review.
References
- J Mol Biol. 1982 Oct 15;161(1):107-33 - PubMed
- J Mol Biol. 1995 Feb 3;245(5):461-6 - PubMed
- Mol Biol Rep. 1982 Nov 30;8(4):185-9 - PubMed
- J Cell Biol. 1983 May;96(5):1471-4 - PubMed
- Proc Natl Acad Sci U S A. 1982 Oct;79(19):5948-52 - PubMed