Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage - PubMed (original) (raw)
. 1996 May 24;258(5):747-62.
doi: 10.1006/jmbi.1996.0284.
Affiliations
- PMID: 8637007
- DOI: 10.1006/jmbi.1996.0284
Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage
L L Bonnycastle et al. J Mol Biol. 1996.
Abstract
The structural requirements for peptide binding to an antibody may be elucidated by probing it with a variety of peptides having different constraints. To this end, we have constructed and screened a panel of peptide libraries displayed by filamentous bacteriophage. The peptides in most of the libraries have the potential for constraint by fixed Cys residues, which have been placed at different sites within a randomized amino acid sequence of varying length. When taken together, the binding data obtained from screening the panel with a given antibody allow one to determine the types of constraints that promote binding, as well as the residues that are critical for binding. We describe the construction of 11, pVIII-displayed, peptide libraries, whose sizes range from 150 million to 10 billion clones. The libraries were screened with a number of polyclonal and monoclonal antibodies against peptides, proteins and carbohydrates. Cross-reactivity with peptides was always found for antibodies produced against peptides, linear epitopes on folded proteins and, surprisingly, carbohydrates, whereas antibodies against discontinuous epitopes on proteins were found less frequently. The implications of these results are discussed in terms of the structural basis for cross-reactivity with peptides.
Similar articles
- Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions.
Stephen CW, Helminen P, Lane DP. Stephen CW, et al. J Mol Biol. 1995 Apr 21;248(1):58-78. doi: 10.1006/jmbi.1995.0202. J Mol Biol. 1995. PMID: 7537340 - Epitope mapping of monoclonal antibodies to keratin 19 using keratin fragments, synthetic peptides and phage peptide libraries.
Böttger V, Stasiak PC, Harrison DL, Mellerick DM, Lane EB. Böttger V, et al. Eur J Biochem. 1995 Jul 15;231(2):475-85. doi: 10.1111/j.1432-1033.1995.tb20721.x. Eur J Biochem. 1995. PMID: 7543411 - Homodimeric peptides displayed by the major coat protein of filamentous phage.
Zwick MB, Shen J, Scott JK. Zwick MB, et al. J Mol Biol. 2000 Jul 7;300(2):307-20. doi: 10.1006/jmbi.2000.3850. J Mol Biol. 2000. PMID: 10873467 - Epitope mapping using bacteriophage peptide libraries.
Lane DP, Stephen CW. Lane DP, et al. Curr Opin Immunol. 1993 Apr;5(2):268-71. doi: 10.1016/0952-7915(93)90016-l. Curr Opin Immunol. 1993. PMID: 7685173 Review. - Surface presentation of protein epitopes using bacteriophage expression systems.
Smith GP. Smith GP. Curr Opin Biotechnol. 1991 Oct;2(5):668-73. doi: 10.1016/0958-1669(91)90032-z. Curr Opin Biotechnol. 1991. PMID: 1370065 Review.
Cited by
- Targeting Plasmodium Life Cycle with Novel Parasite Ligands as Vaccine Antigens.
Khan S, Patel MP, Patni AD, Cha SJ. Khan S, et al. Vaccines (Basel). 2024 Apr 30;12(5):484. doi: 10.3390/vaccines12050484. Vaccines (Basel). 2024. PMID: 38793735 Free PMC article. Review. - Peptide selection via phage display to inhibit _Leishmania_-macrophage interactions.
Verga JBM, Graminha MAS, Jacobs-Lorena M, Cha SJ. Verga JBM, et al. Front Microbiol. 2024 Feb 27;15:1362252. doi: 10.3389/fmicb.2024.1362252. eCollection 2024. Front Microbiol. 2024. PMID: 38476939 Free PMC article. - Plasmodium female gamete surface HSP90 is a key determinant for fertilization.
Cha S-J, Vega-Rodriguez J, Tao D, Kudyba HM, Hanner K, Jacobs-Lorena M. Cha S-J, et al. mBio. 2024 Feb 14;15(2):e0314223. doi: 10.1128/mbio.03142-23. Epub 2023 Dec 22. mBio. 2024. PMID: 38131664 Free PMC article. - Propagation Capacity of Phage Display Peptide Libraries Is Affected by the Length and Conformation of Displayed Peptide.
Kamstrup Sell D, Sinkjaer AW, Bakhshinejad B, Kjaer A. Kamstrup Sell D, et al. Molecules. 2023 Jul 10;28(14):5318. doi: 10.3390/molecules28145318. Molecules. 2023. PMID: 37513190 Free PMC article. - Streamlined phage display library protocols for identification of insect gut binding peptides highlight peptide specificity.
Mishra R, Guo Y, Kumar P, Cantón PE, Tavares CS, Banerjee R, Kuwar S, Bonning BC. Mishra R, et al. Curr Res Insect Sci. 2021 Feb 18;1:100012. doi: 10.1016/j.cris.2021.100012. eCollection 2021. Curr Res Insect Sci. 2021. PMID: 36003592 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources