The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing - PubMed (original) (raw)
. 1996 Jun 1;10(11):1356-68.
doi: 10.1101/gad.10.11.1356.
Affiliations
- PMID: 8647433
- DOI: 10.1101/gad.10.11.1356
Free article
The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing
P Zuo et al. Genes Dev. 1996.
Free article
Abstract
The splicing factor U2AF (U2 snRNP auxiliary factor) is a heterodimer with subunits of 65 and 35 kD (U2AF65 and U2AF35). U2AF65 binds specifically to 3' splice sites, but previous studies failed to demonstrate a function for U2AF35. Here, we report that U2AF35 is required for constitutive splicing and also functions as a mediator of enhancer-dependent splicing. Nuclear extracts deficient in U2AF35 were inactive; however, both constitutive and enhancer-dependent splicing could be restored by the addition of purified recombinant U2AF35. In vitro protein-RNA interaction studies with pre-mRNAs containing either a constitutive or regulated splicing enhancer revealed that U2AF35 directly mediates interactions between U2AF65 and proteins bound to the enhancers. Thus, U2AF35 functions as a bridge between U2AF65 and the enhancer complex to recruit U2AF65 to the adjacent intron.
Similar articles
- A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA.
Tronchère H, Wang J, Fu XD. Tronchère H, et al. Nature. 1997 Jul 24;388(6640):397-400. doi: 10.1038/41137. Nature. 1997. PMID: 9237760 - The role of U2AF35 and U2AF65 in enhancer-dependent splicing.
Graveley BR, Hertel KJ, Maniatis T. Graveley BR, et al. RNA. 2001 Jun;7(6):806-18. doi: 10.1017/s1355838201010317. RNA. 2001. PMID: 11421359 Free PMC article. - Functional recognition of the 3' splice site AG by the splicing factor U2AF35.
Wu S, Romfo CM, Nilsen TW, Green MR. Wu S, et al. Nature. 1999 Dec 16;402(6763):832-5. doi: 10.1038/45590. Nature. 1999. PMID: 10617206 - Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases.
Blencowe BJ. Blencowe BJ. Trends Biochem Sci. 2000 Mar;25(3):106-10. doi: 10.1016/s0968-0004(00)01549-8. Trends Biochem Sci. 2000. PMID: 10694877 Review. - Initial splice-site recognition and pairing during pre-mRNA splicing.
Reed R. Reed R. Curr Opin Genet Dev. 1996 Apr;6(2):215-20. doi: 10.1016/s0959-437x(96)80053-0. Curr Opin Genet Dev. 1996. PMID: 8722179 Review.
Cited by
- Cellular sex throughout the organism underlies somatic sexual differentiation.
Hérault C, Pihl T, Hudry B. Hérault C, et al. Nat Commun. 2024 Aug 13;15(1):6925. doi: 10.1038/s41467-024-51228-6. Nat Commun. 2024. PMID: 39138201 Free PMC article. - Exonic splicing code and coordination of divalent metals in proteins.
Bakhtiar D, Vondraskova K, Pengelly RJ, Chivers M, Kralovicova J, Vorechovsky I. Bakhtiar D, et al. Nucleic Acids Res. 2024 Feb 9;52(3):1090-1106. doi: 10.1093/nar/gkad1161. Nucleic Acids Res. 2024. PMID: 38055834 Free PMC article. - Regulation of Flowering Time and Other Developmental Plasticities by 3' Splicing Factor-Mediated Alternative Splicing in Arabidopsis thaliana.
Lee KC, Kim YC, Kim JK, Lee H, Lee JH. Lee KC, et al. Plants (Basel). 2023 Oct 9;12(19):3508. doi: 10.3390/plants12193508. Plants (Basel). 2023. PMID: 37836248 Free PMC article. Review. - Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.
Black CS, Whelan TA, Garside EL, MacMillan AM, Fast NM, Rader SD. Black CS, et al. RNA. 2023 May;29(5):531-550. doi: 10.1261/rna.079273.122. Epub 2023 Feb 3. RNA. 2023. PMID: 36737103 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases