Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo - PubMed (original) (raw)
Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo
G A Michelotti et al. Mol Cell Biol. 1996 Jun.
Abstract
Transcription activation and repression of eukaryotic genes are associated with conformational and topological changes of the DNA and chromatin, altering the spectrum of proteins associated with an active gene. Segments of the human c-myc gene possessing non-B structure in vivo located with enzymatic and chemical probes. Sites hypertensive to cleavage with single-strand-specific S1 nuclease or the single-strand-selective agent potassium permanganate included the major promoters P1 and P2 as well as the far upstream sequence element (FUSE) and CT elements, which bind, respectively, the single-strand-specific factors FUSE-binding protein and heterogeneous nuclear ribonucleoprotein K in vitro. Active and inactive c-myc genes yielded different patterns of S1 nuclease and permanganate sensitivity, indicating alternative chromatin configurations of active and silent genes. The melting of specific cis elements of active c-myc genes in vivo suggested that transcriptionally associated torsional strain might assist strand separation and facilitate factor binding. Therefore, the interaction of FUSE-binding protein and heterogeneous nuclear ribonucleoprotein K with supercoiled DNA was studied. Remarkably, both proteins recognize their respective elements torsionally strained but not as liner duplexes. Single-strand- or supercoil-dependent gene regulatory proteins may directly link alterations in DNA conformation and topology with changes in gene expression.
Similar articles
- Activating transcription from single stranded DNA.
Tomonaga T, Levens D. Tomonaga T, et al. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5830-5. doi: 10.1073/pnas.93.12.5830. Proc Natl Acad Sci U S A. 1996. PMID: 8650178 Free PMC article. - Heterogeneous nuclear ribonucleoprotein K is a transcription factor.
Michelotti EF, Michelotti GA, Aronsohn AI, Levens D. Michelotti EF, et al. Mol Cell Biol. 1996 May;16(5):2350-60. doi: 10.1128/MCB.16.5.2350. Mol Cell Biol. 1996. PMID: 8628302 Free PMC article. - A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif.
Duncan R, Bazar L, Michelotti G, Tomonaga T, Krutzsch H, Avigan M, Levens D. Duncan R, et al. Genes Dev. 1994 Feb 15;8(4):465-80. doi: 10.1101/gad.8.4.465. Genes Dev. 1994. PMID: 8125259 - Structure of transcriptionally active chromatin.
Yaniv M, Cereghini S. Yaniv M, et al. CRC Crit Rev Biochem. 1986;21(1):1-26. doi: 10.3109/10409238609113607. CRC Crit Rev Biochem. 1986. PMID: 3015490 Review. - The importance of being supercoiled: how DNA mechanics regulate dynamic processes.
Baranello L, Levens D, Gupta A, Kouzine F. Baranello L, et al. Biochim Biophys Acta. 2012 Jul;1819(7):632-8. doi: 10.1016/j.bbagrm.2011.12.007. Epub 2012 Jan 3. Biochim Biophys Acta. 2012. PMID: 22233557 Free PMC article. Review.
Cited by
- FUBP1 in human cancer: Characteristics, functions, and potential applications.
Zhang F, Xiong Q, Wang M, Cao X, Zhou C. Zhang F, et al. Transl Oncol. 2024 Oct;48:102066. doi: 10.1016/j.tranon.2024.102066. Epub 2024 Jul 26. Transl Oncol. 2024. PMID: 39067088 Free PMC article. Review. - Negative Electron Transfer Collision-Induced Dissociation of G-Quadruplexes: Uncovering the Guanine Radical Anion Loss Pathway.
Brundridge NM, Fritz JM, Dickerhoff J, Yang D, McLuckey SA. Brundridge NM, et al. J Am Soc Mass Spectrom. 2024 Apr 3;35(4):756-766. doi: 10.1021/jasms.3c00443. Epub 2024 Mar 8. J Am Soc Mass Spectrom. 2024. PMID: 38456425 - The RNA-binding KH-domain in the unique transcription factor of the malaria parasite is responsible for its transcriptional regulatory activity.
Komaki-Yasuda K, Kano S. Komaki-Yasuda K, et al. PLoS One. 2023 Dec 21;18(12):e0296165. doi: 10.1371/journal.pone.0296165. eCollection 2023. PLoS One. 2023. PMID: 38128025 Free PMC article. - MYC function and regulation in physiological perspective.
Jha RK, Kouzine F, Levens D. Jha RK, et al. Front Cell Dev Biol. 2023 Oct 24;11:1268275. doi: 10.3389/fcell.2023.1268275. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 37941901 Free PMC article. Review. - G-Quadruplex DNA and Other Non-Canonical B-Form DNA Motifs Influence Productive and Latent HIV-1 Integration and Reactivation Potential.
Ajoge HO, Kohio HP, Paparisto E, Coleman MD, Wong K, Tom SK, Bain KL, Berry CC, Arts EJ, Barr SD. Ajoge HO, et al. Viruses. 2022 Nov 11;14(11):2494. doi: 10.3390/v14112494. Viruses. 2022. PMID: 36423103 Free PMC article.
References
- EMBO J. 1992 Sep;11(9):3307-14 - PubMed
- EMBO J. 1992 Dec;11(12):4653-63 - PubMed
- EMBO J. 1995 Jul 3;14(13):3215-25 - PubMed
- Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7125-9 - PubMed
- Nucleic Acids Res. 1995 Sep 11;23(17):3373-9 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources