ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism - PubMed (original) (raw)
. 1996 May 1;10(9):1096-107.
doi: 10.1101/gad.10.9.1096.
Affiliations
- PMID: 8654925
- DOI: 10.1101/gad.10.9.1096
Free article
ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism
J B Kim et al. Genes Dev. 1996.
Free article
Abstract
Adipocyte determination and differentiation-dependent factor 1 (ADD1) is a member of the basic helix-loop-helix leucine zipper (bHLH-LZ) family of transcription factors that binds to two distinct DNA sequences and has been associated with both adipocyte development and cholesterol homeostasis (where it has been termed SREBP1). To investigate the biological role of ADD1, we expressed wild-type and dominant negative forms of this protein with retroviral vectors in preadipocytes and nonadipogenic cells. A dominant-negative form of ADD1 with a point mutation in the DNA-binding domain sharply represses the differentiation of 3T3-L1 cells as observed morphologically or by the expression of adipocyte-specific mRNAs. When NIH-3T3 cells ectopically expressing ADD1 are cultured under hormonal conditions not favoring differentiation, they do not overtly differentiate but still activate expression of mRNAs for fatty acid synthase (FAS) and lipoprotein lipase (LPL), two key genes that regulate fatty acid metabolism. Under culture conditions permissive for differentiation including a PPAR activator, 15%-20% of the cells expressing ADD1 undergo adipogenesis while 2%-3% of cells containing a control vector differentiate. Simultaneous expression of ADD1 with PPARgamma increases the transcriptional activity of this adipogenic nuclear hormone receptor, suggesting involvement of ADD1 in this pathway. These data indicate that ADD1 plays an important role in fat cell gene expression and differentiation, and suggest that it may function by augmenting a step in PPARgamma-mediated transcription.
Similar articles
- Dual DNA binding specificity of ADD1/SREBP1 controlled by a single amino acid in the basic helix-loop-helix domain.
Kim JB, Spotts GD, Halvorsen YD, Shih HM, Ellenberger T, Towle HC, Spiegelman BM. Kim JB, et al. Mol Cell Biol. 1995 May;15(5):2582-8. doi: 10.1128/MCB.15.5.2582. Mol Cell Biol. 1995. PMID: 7739539 Free PMC article. - Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1.
Kim JB, Sarraf P, Wright M, Yao KM, Mueller E, Solanes G, Lowell BB, Spiegelman BM. Kim JB, et al. J Clin Invest. 1998 Jan 1;101(1):1-9. doi: 10.1172/JCI1411. J Clin Invest. 1998. PMID: 9421459 Free PMC article. - Control of adipocyte differentiation by CCAAT/enhancer binding protein alpha (C/EBP alpha).
Lane MD, Lin FT, MacDougald OA, Vasseur-Cognet M. Lane MD, et al. Int J Obes Relat Metab Disord. 1996 Mar;20 Suppl 3:S91-6. Int J Obes Relat Metab Disord. 1996. PMID: 8680485 Review. - Regulation of adipocyte gene expression and differentiation by peroxisome proliferator activated receptor gamma.
Tontonoz P, Hu E, Spiegelman BM. Tontonoz P, et al. Curr Opin Genet Dev. 1995 Oct;5(5):571-6. doi: 10.1016/0959-437x(95)80025-5. Curr Opin Genet Dev. 1995. PMID: 8664544 Review.
Cited by
- Lipid Storage, Lipolysis, and Lipotoxicity in Obesity.
Engin A. Engin A. Adv Exp Med Biol. 2024;1460:97-129. doi: 10.1007/978-3-031-63657-8_4. Adv Exp Med Biol. 2024. PMID: 39287850 Review. - A Closer Look into White Adipose Tissue Biology and the Molecular Regulation of Stem Cell Commitment and Differentiation.
Dowker-Key PD, Jadi PK, Gill NB, Hubbard KN, Elshaarrawi A, Alfatlawy ND, Bettaieb A. Dowker-Key PD, et al. Genes (Basel). 2024 Aug 2;15(8):1017. doi: 10.3390/genes15081017. Genes (Basel). 2024. PMID: 39202377 Free PMC article. Review. - The Role of Adipose Tissue and Nutrition in the Regulation of Adiponectin.
Baldelli S, Aiello G, Mansilla Di Martino E, Campaci D, Muthanna FMS, Lombardo M. Baldelli S, et al. Nutrients. 2024 Jul 26;16(15):2436. doi: 10.3390/nu16152436. Nutrients. 2024. PMID: 39125318 Free PMC article. Review. - Navigating the Adipocyte Precursor Niche: Cell-Cell Interactions, Regulatory Mechanisms and Implications for Adipose Tissue Homeostasis.
Kesharwani D, Brown AC. Kesharwani D, et al. J Cell Signal. 2024;5(2):65-86. doi: 10.33696/signaling.5.114. J Cell Signal. 2024. PMID: 38826152 Free PMC article. - Effects of Chronic Sleep Restriction on Transcriptional Sirtuin 1 Signaling Regulation in Male Mice White Adipose Tissue.
Rendine M, Cocci P, de Vivo L, Bellesi M, Palermo FA. Rendine M, et al. Curr Issues Mol Biol. 2024 Mar 7;46(3):2144-2154. doi: 10.3390/cimb46030138. Curr Issues Mol Biol. 2024. PMID: 38534754 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous