Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: evidence for rolling-circle replication in a hyperthermophile - PubMed (original) (raw)

Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: evidence for rolling-circle replication in a hyperthermophile

G Erauso et al. J Bacteriol. 1996 Jun.

Abstract

The plasmid pGT5 (3,444 bp) from the hyperthermophilic archaeon Pyrococcus abyssi GE5 has been completely sequenced. Two major open reading frames with a good coding probability are located on the same strand and cover 85% of the total sequence. The larger open reading frame encodes a putative polypeptide which exhibits sequence similarity with Rep proteins of plasmids using the rolling-circle mechanism for replication. Upstream of this open reading frame, we have detected an 11-bp motif identical to the double-stranded origin of several bacterial plasmids that replicate via the rolling-circle mechanism. A putative single-stranded origin exhibits similarities both to bacterial primosome-dependent single-stranded initiation sites and to bacterial primase (dnaG) start sites. A single-stranded form of pGT5 corresponding to the plus strand was detected in cells of P. abyssi. These data indicate that pGT5 replicates via the rolling-circle mechanism and suggest that members of the domain Archaea contain homologs of several bacterial proteins involved in chromosomal DNA replication. Phylogenetic analysis of Rep proteins from rolling-circle replicons suggest that diverse families diverged before the separation of the domains Archaea, Bacteria, and Eucarya.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1989 Dec 25;17(24):10501 -PubMed
    1. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):859-63 -PubMed
    1. Annu Rev Microbiol. 1989;43:537-65 -PubMed
    1. Trends Biotechnol. 1992 Nov;10(11):395-402 -PubMed
    1. J Mol Biol. 1994 Apr 8;237(4):388-400 -PubMed

Publication types

MeSH terms

LinkOut - more resources