Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates - PubMed (original) (raw)
Enzymatic synthesis of a quorum-sensing autoinducer through use of defined substrates
M I Moré et al. Science. 1996.
Abstract
Many bacteria, including several pathogens of plants and humans, use a pheromone called an autoinducer to regulate gene expression in a cell density-dependent manner. Agrobacterium autoinducer [AAI, N-(3-oxo-octanoyl)-L-homoserine lactone] of A. tumefaciens is synthesized by the Tral protein, which is encoded by the tumor-inducing plasmid. Purified hexahistidinyl-Tral (H6-Tral) used S-adenosylmethionine to make the homoserine lactone moiety of AAI, but did not use related compounds. H6-Tral used 3-oxo-octanoyl-acyl carrier protein to make the 3-oxo-octanoyl moiety of AAI, but did not use 3-oxo-octanoyl-coenzyme A. These results demonstrate the enzymatic synthesis of an autoinducer through the use of purified substrates.
Comment in
- Bacteria also vote.
Kaiser D. Kaiser D. Science. 1996 Jun 14;272(5268):1598-9. doi: 10.1126/science.272.5268.1598. Science. 1996. PMID: 8658131 No abstract available.
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