Roles of XPG and XPF/ERCC1 endonucleases in UV-induced immunostaining of PCNA in fibroblasts - PubMed (original) (raw)
. 1996 Jul 10;226(1):126-32.
doi: 10.1006/excr.1996.0210.
Affiliations
- PMID: 8660947
- DOI: 10.1006/excr.1996.0210
Roles of XPG and XPF/ERCC1 endonucleases in UV-induced immunostaining of PCNA in fibroblasts
M Miura et al. Exp Cell Res. 1996.
Abstract
To investigate the relationship between proliferating cell nuclear antigen (PCNA) complex formation and dual incisions in the nucleotide excision repair (NER) process, xeroderma pigmentosum group G (XP-G), XP-F, and XP-G equivalent mouse UV-sensitive mutant ERCC group 5 cells were utilized as a model in this study. These cells are deficient in endonucleases related to 3' (XP-G and ERCC group 5) or 5' (XP-F) incision of the DNA lesions in the NER process. PCNA complex formation was detected by an indirect immunofluorescence method after the cells were fixed in methanol. When Sps1 (XP-G) and XL216-7 (ERCC group 5) cells were UV irradiated, neither of them showed PCNA staining. In contrast, SFN4 (a human normal strain) and heterokaryons of Sps1 and XP96TO (XP-A) cells fused by polyethylene glycol treatment showed PCNA staining following UV irradiation. Furthermore, XLgfPAneo1 cells, derived from XL216-7 cells transfected with a plasmid containing mouse ERCC5 (xpg) cDNA, also restored staining and UV sensitivity. On the other hand, we observed a very faint PCNA staining in XP2YO (XP-F) cells, expressing no detectable ERCC1 or XPF protein, after UV irradiation. X rays induced PCNA staining in all cell lines with a similar staining pattern, and radiosensitivity was exactly the same between XL216-7 and XLgfPAneo1 cells. These results may have implications for the NER process in vivo in that coordinately occurring dual incisions by XPG and XPF/ERCC1 proteins play an important role in inducing PCNA complex formation, but the step may not be required for PCNA-dependent repair of X-ray-induced DNA damage.
Similar articles
- Detection of nucleotide excision repair incisions in human fibroblasts by immunostaining for PCNA.
Aboussekhra A, Wood RD. Aboussekhra A, et al. Exp Cell Res. 1995 Dec;221(2):326-32. doi: 10.1006/excr.1995.1382. Exp Cell Res. 1995. PMID: 7493631 - Xeroderma pigmentosum and molecular cloning of DNA repair genes.
Boulikas T. Boulikas T. Anticancer Res. 1996 Mar-Apr;16(2):693-708. Anticancer Res. 1996. PMID: 8687116 Review. - Multiple roles of the ERCC1-XPF endonuclease in DNA repair and resistance to anticancer drugs.
Kirschner K, Melton DW. Kirschner K, et al. Anticancer Res. 2010 Sep;30(9):3223-32. Anticancer Res. 2010. PMID: 20944091 Review.
Cited by
- Porcine alveolar macrophages host proteins interacting with African swine fever virus p72.
Weng Z, Zheng X, Liang Y, Chen X, Peng Q, Zhang G, Gong L, Zheng Z. Weng Z, et al. Front Microbiol. 2024 Feb 28;15:1370417. doi: 10.3389/fmicb.2024.1370417. eCollection 2024. Front Microbiol. 2024. PMID: 38481793 Free PMC article. - Quantification of excision repair cross-complementing group 1 and survival in p16-negative squamous cell head and neck cancers.
Mehra R, Zhu F, Yang DH, Cai KQ, Weaver J, Singh MK, Nikonova AS, Golemis EA, Flieder DB, Cooper HS, Lango M, Ridge JA, Burtness B. Mehra R, et al. Clin Cancer Res. 2013 Dec 1;19(23):6633-43. doi: 10.1158/1078-0432.CCR-13-0152. Epub 2013 Oct 2. Clin Cancer Res. 2013. PMID: 24088734 Free PMC article. - The comings and goings of nucleotide excision repair factors on damaged DNA.
Riedl T, Hanaoka F, Egly JM. Riedl T, et al. EMBO J. 2003 Oct 1;22(19):5293-303. doi: 10.1093/emboj/cdg489. EMBO J. 2003. PMID: 14517266 Free PMC article. - Interaction between proliferating cell nuclear antigen and JUN-activation-domain-binding protein 1 in the meristem of rice, Oryza sativa L.
Yamamoto T, Kimura S, Mori Y, Uchiyama Y, Ishibashi T, Hashimoto J, Sakaguchi K. Yamamoto T, et al. Planta. 2003 Jun;217(2):175-83. doi: 10.1007/s00425-003-0981-z. Epub 2003 Mar 15. Planta. 2003. PMID: 12783325 - Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells.
Balajee AS, Geard CR. Balajee AS, et al. Nucleic Acids Res. 2001 Mar 15;29(6):1341-51. doi: 10.1093/nar/29.6.1341. Nucleic Acids Res. 2001. PMID: 11239001 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous