TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes - PubMed (original) (raw)
TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes
K Y Yankulov et al. Mol Cell Biol. 1996 Jul.
Abstract
We investigated the role of TFIIH in transcription by RNA polymerase II (pol II) in vivo by microinjection of antibodies against this factor into Xenopus oocytes. Five different antibodies directed against four subunits of TFIIH were tested for effects on transcription of coinjected human immunodeficiency virus type 2 and c-myc templates. Each of these antibodies severely reduced the efficiency of elongation through human immunodeficiency virus type 2 and c-myc terminator elements. In contrast, an anti-TFIIB antibody did not inhibit elongation. Anti-TFIIH antibodies also had a much smaller inhibitory effect on total transcription than did anti-TFIIB or anti-pol II large subunit. Three inhibitors of TFIIH kinase activity, H-7, H-8, and dichlororibofuranosylbenzimidazole (DRB), inhibited elongation similarly to anti-TFIIH antibodies. These results strongly suggest a role for TFIIH in the stimulation of transcriptional elongation in vivo.
Similar articles
- Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase II.
Drapkin R, Reardon JT, Ansari A, Huang JC, Zawel L, Ahn K, Sancar A, Reinberg D. Drapkin R, et al. Nature. 1994 Apr 21;368(6473):769-72. doi: 10.1038/368769a0. Nature. 1994. PMID: 8152490 - The transcriptional elongation inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase.
Yankulov K, Yamashita K, Roy R, Egly JM, Bentley DL. Yankulov K, et al. J Biol Chem. 1995 Oct 13;270(41):23922-5. doi: 10.1074/jbc.270.41.23922. J Biol Chem. 1995. PMID: 7592583 - The multiple roles of transcription/repair factor TFIIH.
Svejstrup JQ, Vichi P, Egly JM. Svejstrup JQ, et al. Trends Biochem Sci. 1996 Sep;21(9):346-50. Trends Biochem Sci. 1996. PMID: 8870499 Review. - TFIIH: a link between transcription, DNA repair and cell cycle regulation.
Seroz T, Hwang JR, Moncollin V, Egly JM. Seroz T, et al. Curr Opin Genet Dev. 1995 Apr;5(2):217-21. doi: 10.1016/0959-437x(95)80011-5. Curr Opin Genet Dev. 1995. PMID: 7613092 Review.
Cited by
- Poly(A)-driven and poly(A)-assisted termination: two different modes of poly(A)-dependent transcription termination.
Yeung G, Choi LM, Chao LC, Park NJ, Liu D, Jamil A, Martinson HG. Yeung G, et al. Mol Cell Biol. 1998 Jan;18(1):276-89. doi: 10.1128/MCB.18.1.276. Mol Cell Biol. 1998. PMID: 9418875 Free PMC article. - Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae.
Tijsterman M, Verhage RA, van de Putte P, Tasseron-de Jong JG, Brouwer J. Tijsterman M, et al. Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8027-32. doi: 10.1073/pnas.94.15.8027. Proc Natl Acad Sci U S A. 1997. PMID: 9223308 Free PMC article. - An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae.
Archambault J, Chambers RS, Kobor MS, Ho Y, Cartier M, Bolotin D, Andrews B, Kane CM, Greenblatt J. Archambault J, et al. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14300-5. doi: 10.1073/pnas.94.26.14300. Proc Natl Acad Sci U S A. 1997. PMID: 9405607 Free PMC article. - Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro.
Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H. Wada T, et al. EMBO J. 1998 Dec 15;17(24):7395-403. doi: 10.1093/emboj/17.24.7395. EMBO J. 1998. PMID: 9857195 Free PMC article. - Promoter proximal pausing on genes in metazoans.
Gilmour DS. Gilmour DS. Chromosoma. 2009 Feb;118(1):1-10. doi: 10.1007/s00412-008-0182-4. Epub 2008 Oct 2. Chromosoma. 2009. PMID: 18830703 Review.
References
- Genes Dev. 1995 Jun 15;9(12):1479-90 - PubMed
- J Mol Biol. 1995 Jun 9;249(3):535-44 - PubMed
- Nature. 1995 Aug 24;376(6542):709-12 - PubMed
- Cell. 1995 Oct 6;83(1):47-57 - PubMed
- J Biol Chem. 1995 Oct 13;270(41):23922-5 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials