Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation - PubMed (original) (raw)

. 1996 Jun 17;15(12):3153-63.

Affiliations

PMID: 8670816
PMCID: PMC450258

Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation

C Cogoni et al. EMBO J. 1996.

Abstract

The molecular mechanisms involved in transgene-induced gene silencing ('quelling') in Neurospora crassa were investigated using the carotenoid biosynthetic gene albino-1 (al-1) as a visual marker. Deletion derivatives of the al-1 gene showed that a transgene must contain at least approximately 132 bp of sequences homologous to the transcribed region of the native gene in order to induce quelling. Transgenes containing only al-1 promoter sequences do not cause quelling. Specific sequences are not required for gene silencing, as different regions of the al-1 gene produced quelling. A mutant defective in cytosine methylation (dim-2) exhibited normal frequencies and degrees of silencing, indicating that cytosine methylation is not responsible for quelling, despite the fact that methylation of transgene sequences frequently is correlated with silencing. Silencing was shown to be a dominant trait, operative in heterokaryotic strains containing a mixture of transgenic and non-transgenic nuclei. This result indicates that a diffusable, trans-acting molecule is involved in quelling. A transgene-derived, sense RNA was detected in quelled strains and was found to be absent in their revertants. These data are consistent with a model in which an RNA-DNA or RNA-RNA interaction is involved in transgene-induced gene silencing in Neurospora.

PubMed Disclaimer

Cited by

RNA Interference in Insects: From a Natural Mechanism of Gene Expression Regulation to a Biotechnological Crop Protection Promise.
Ortolá B, Daròs JA. Ortolá B, et al. Biology (Basel). 2024 Feb 21;13(3):137. doi: 10.3390/biology13030137. Biology (Basel). 2024. PMID: 38534407 Free PMC article. Review.
RNAi turns 25:contributions and challenges in insect science.
Palli SR. Palli SR. Front Insect Sci. 2023 Oct 4;3:1209478. doi: 10.3389/finsc.2023.1209478. eCollection 2023. Front Insect Sci. 2023. PMID: 38469536 Free PMC article. Review.
CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda.
Gouda MNR, Jeevan H, Shashank HG. Gouda MNR, et al. Mol Biol Rep. 2023 Dec 12;51(1):13. doi: 10.1007/s11033-023-08986-1. Mol Biol Rep. 2023. PMID: 38085335 Review.
Magnaporthe oryzae pathotype Triticum (MoT) can act as a heterologous expression system for fungal effectors with high transcript abundance in wheat.
Jensen C, Saunders DGO. Jensen C, et al. Sci Rep. 2023 Jan 3;13(1):108. doi: 10.1038/s41598-022-27030-z. Sci Rep. 2023. PMID: 36596834 Free PMC article.
Progression of geminivirus-induced transgene silencing is associated with transgene methylation.
Rodman MK, Yadav NS, Artus NN. Rodman MK, et al. New Phytol. 2002 Sep;155(3):461-468. doi: 10.1046/j.1469-8137.2002.00467.x. New Phytol. 2002. PMID: 33873315

References

1. Mol Cell Biol. 1986 Jul;6(7):2452-61 - PubMed
1. Annu Rev Genet. 1990;24:579-613 - PubMed
1. Cold Spring Harb Symp Quant Biol. 1993;58:323-9 - PubMed
1. Proc Natl Acad Sci U S A. 1990 Nov;87(22):9057-61 - PubMed
1. Genetics. 1995 Apr;139(4):1533-44 - PubMed

Transgene silencing of the al-1 gene in vegetative cells of Neurospora is mediated by a cytoplasmic effector and does not depend on DNA-DNA interactions or DNA methylation - PubMed (original) (raw)