Requirements for dE2F function in proliferating cells and in post-mitotic differentiating cells - PubMed (original) (raw)
. 1996 Jul 15;15(14):3676-83.
Affiliations
- PMID: 8670871
- PMCID: PMC452016
Requirements for dE2F function in proliferating cells and in post-mitotic differentiating cells
A Brook et al. EMBO J. 1996.
Abstract
The transcription factor E2F is a target of the retinoblastoma tumor suppressor protein (pRB) and may mediate pRB regulation of S phase entry in mammalian cells. The recent identification of mutant alleles of the Drosophila E2F gene (dE2F) has shown that dE2F is required for embryogenesis. dE2F-mutant embryos lack a co-ordinated program of gene expression which accompanies S phase entry and DNA synthesis declines to levels that are barely detectable. We have investigated the role of the dE2F gene at later stages of development. dE2F is expressed in several larval tissues and is required for cell proliferation in the eye imaginal disc. Surprisingly, dE2F expression persists in post-mitotic cells of the eye disc of third-instar larvae. The loss of dE2F function in these cells causes a novel phenotype, characterized by loss of photoreceptors and abnormal rhabdomere cell morphology. These results show that dE2F is required at multiple stages of development and suggest that E2F may have an important function in post-mitotic cells in addition to its role during cell proliferation.
Similar articles
- Ectopic expression of dE2F and dDP induces cell proliferation and death in the Drosophila eye.
Du W, Xie JE, Dyson N. Du W, et al. EMBO J. 1996 Jul 15;15(14):3684-92. EMBO J. 1996. PMID: 8670872 Free PMC article. - RBF, a novel RB-related gene that regulates E2F activity and interacts with cyclin E in Drosophila.
Du W, Vidal M, Xie JE, Dyson N. Du W, et al. Genes Dev. 1996 May 15;10(10):1206-18. doi: 10.1101/gad.10.10.1206. Genes Dev. 1996. PMID: 8675008 - dE2F2, a novel E2F-family transcription factor in Drosophila melanogaster.
Sawado T, Yamaguchi M, Nishimoto Y, Ohno K, Sakaguchi K, Matsukage A. Sawado T, et al. Biochem Biophys Res Commun. 1998 Oct 20;251(2):409-15. doi: 10.1006/bbrc.1998.9407. Biochem Biophys Res Commun. 1998. PMID: 9792788 - Activity of the retinoblastoma family proteins, pRB, p107, and p130, during cellular proliferation and differentiation.
Sidle A, Palaty C, Dirks P, Wiggan O, Kiess M, Gill RM, Wong AK, Hamel PA. Sidle A, et al. Crit Rev Biochem Mol Biol. 1996 Jun;31(3):237-71. doi: 10.3109/10409239609106585. Crit Rev Biochem Mol Biol. 1996. PMID: 8817077 Review. - Growth regulation by the E2F and DP transcription factor families.
Yamasaki L. Yamasaki L. Results Probl Cell Differ. 1998;22:199-227. doi: 10.1007/978-3-540-69686-5_9. Results Probl Cell Differ. 1998. PMID: 9670325 Review. No abstract available.
Cited by
- A role for Ebi in neuronal cell cycle control.
Boulton SJ, Brook A, Staehling-Hampton K, Heitzler P, Dyson N. Boulton SJ, et al. EMBO J. 2000 Oct 16;19(20):5376-86. doi: 10.1093/emboj/19.20.5376. EMBO J. 2000. PMID: 11032805 Free PMC article. - Ectopic expression of DREF induces DNA synthesis, apoptosis, and unusual morphogenesis in the Drosophila eye imaginal disc: possible interaction with Polycomb and trithorax group proteins.
Hirose F, Ohshima N, Shiraki M, Inoue YH, Taguchi O, Nishi Y, Matsukage A, Yamaguchi M. Hirose F, et al. Mol Cell Biol. 2001 Nov;21(21):7231-42. doi: 10.1128/MCB.21.21.7231-7242.2001. Mol Cell Biol. 2001. PMID: 11585906 Free PMC article. - Ectopic expression of dE2F and dDP induces cell proliferation and death in the Drosophila eye.
Du W, Xie JE, Dyson N. Du W, et al. EMBO J. 1996 Jul 15;15(14):3684-92. EMBO J. 1996. PMID: 8670872 Free PMC article. - Collaborative role of E2F transcriptional activity and G1 cyclindependent kinase activity in the induction of S phase.
Leone G, DeGregori J, Jakoi L, Cook JG, Nevins JR. Leone G, et al. Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6626-31. doi: 10.1073/pnas.96.12.6626. Proc Natl Acad Sci U S A. 1999. PMID: 10359762 Free PMC article. - Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2.
Stevaux O, Dimova D, Frolov MV, Taylor-Harding B, Morris E, Dyson N. Stevaux O, et al. EMBO J. 2002 Sep 16;21(18):4927-37. doi: 10.1093/emboj/cdf501. EMBO J. 2002. PMID: 12234932 Free PMC article.
References
- Genes Dev. 1993 Jul;7(7A):1111-25 - PubMed
- BTTA Rev. 1973 Mar;3(1):1-12 - PubMed
- Cell. 1992 Sep 18;70(6):993-1006 - PubMed
- Cell. 1992 Jul 24;70(2):337-50 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases