Applications of heteroduplex analysis for mutation detection in disease genes - PubMed (original) (raw)

Review

Applications of heteroduplex analysis for mutation detection in disease genes

D Glavac et al. Hum Mutat. 1995.

Abstract

Double-stranded heteroduplex molecules that form between a mutant and wild-type DNA strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the HA method have been developed that increase the sensitivity of detection of single-base pair alterations.

PubMed Disclaimer

Similar articles

• CFTR mutation detection by multiplex heteroduplex (mHET) analysis on MDE gel.
  Zielenski J, Aznarez I, Onay T, Tzounzouris J, Markiewicz D, Tsui LC. Zielenski J, et al. Methods Mol Med. 2002;70:3-19. doi: 10.1385/1-59259-187-6:03. Methods Mol Med. 2002. PMID: 11917532 No abstract available.
• Mutation detection by denaturing DNA chromatography using fluorescently labeled polymerase chain reaction products.
  Hecker KH, Taylor PD, Gjerde DT. Hecker KH, et al. Anal Biochem. 1999 Aug 1;272(2):156-64. doi: 10.1006/abio.1999.4171. Anal Biochem. 1999. PMID: 10415084
• Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection.
  Nataraj AJ, Olivos-Glander I, Kusukawa N, Highsmith WE Jr. Nataraj AJ, et al. Electrophoresis. 1999 Jun;20(6):1177-85. doi: 10.1002/(SICI)1522-2683(19990101)20:6<1177::AID-ELPS1177>3.0.CO;2-2. Electrophoresis. 1999. PMID: 10380757 Review.
• Solid phase fluorescent sequencing of the CFTR gene.
  Cuppens H, Cassiman JJ. Cuppens H, et al. Methods Mol Biol. 2001;167:63-88. doi: 10.1385/1-59259-113-2:063. Methods Mol Biol. 2001. PMID: 11265322 Review. No abstract available.

Cited by

• PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping.
  Beaulieu M, Larson GP, Geller L, Flanagan SD, Krontiris TG. Beaulieu M, et al. Nucleic Acids Res. 2001 Mar 1;29(5):1114-24. doi: 10.1093/nar/29.5.1114. Nucleic Acids Res. 2001. PMID: 11222761 Free PMC article.
• Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: comparison with denaturing gradient gel electrophoresis and nucleotide sequencing.
  Körkkö J, Annunen S, Pihlajamaa T, Prockop DJ, Ala-Kokko L. Körkkö J, et al. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1681-5. doi: 10.1073/pnas.95.4.1681. Proc Natl Acad Sci U S A. 1998. PMID: 9465076 Free PMC article.
• A novel denaturing heteroduplex tracking assay for genotypic prediction of HIV-1 tropism.
  Shi B, Weiser B, Styer LM, Kemal K, Brunner C, Anastos K, Burger H. Shi B, et al. J Virol Methods. 2012 Oct;185(1):108-17. doi: 10.1016/j.jviromet.2012.06.013. Epub 2012 Jun 21. J Virol Methods. 2012. PMID: 22728273 Free PMC article.
• PCR-SSCP: a method for the molecular analysis of genetic diseases.
  Kakavas VK, Plageras P, Vlachos TA, Papaioannou A, Noulas VA. Kakavas VK, et al. Mol Biotechnol. 2008 Feb;38(2):155-63. doi: 10.1007/s12033-007-9006-7. Epub 2007 Oct 13. Mol Biotechnol. 2008. PMID: 18219595 Review.
• Identification of Porphyromonas gingivalis strains by heteroduplex analysis and detection of multiple strains.
  Leys EJ, Smith JH, Lyons SR, Griffen AL. Leys EJ, et al. J Clin Microbiol. 1999 Dec;37(12):3906-11. doi: 10.1128/JCM.37.12.3906-3911.1999. J Clin Microbiol. 1999. PMID: 10565905 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information