Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts - PubMed (original) (raw)
. 1996 Aug 1;382(6590):420-5.
doi: 10.1038/382420a0.
Affiliations
- PMID: 8684481
- DOI: 10.1038/382420a0
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
R Heald et al. Nature. 1996.
Abstract
Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts. Bipolar spindles assemble in the absence of centrosomes and kinetochores, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm. Microtubules nucleated at dispersed sites with random polarity rearrange into two arrays of uniform polarity. Spindle-pole formation requires cytoplasmic dynein-dependent translocation of microtubules across one another. It is proposed that spindles form in the absence of centrosomes by motor-dependent sorting of microtubules according to their polarity.
Comment in
- Look ma, no chromosomes!
Hyams J. Hyams J. Nature. 1996 Aug 1;382(6590):397-8. doi: 10.1038/382397a0. Nature. 1996. PMID: 8684477 No abstract available.
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