Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus - PubMed (original) (raw)
Comparative Study
Humoral and cell-mediated autoimmunity in allergy to Aspergillus fumigatus
R Crameri et al. J Exp Med. 1996.
Abstract
A cDNA encoding an allergenic protein was isolated from an Aspergillus fumigatus (A. fumigatus) cDNA library displayed on the surface of filamentous phage. Serum immunoglobulin E (IgE) from A. fumigatus-sensitized individuals was used to enrich phage-expressing gene products binding to IgE. One of the cDNAs encoded a 26.7-kD protein that was identified as a manganese superoxide dismutase (MnSOD) sharing 51.5% identity and 67.2% homology to the corresponding human enzyme. Both human and A. fumigatus MnSOD coding sequences were expressed in Escherichia coli as [His]6-tagged fusion proteins and purified by Ni(2+)-chelate affinity chromatography. The two recombinant MnSODs were both recognized by IgE antibodies from subjects allergic to the A. fumigatus MnSOD and elicited specific immediate type allergic skin reactions in these individuals. Moreover, both human and A. fumigatus MnSOD induced proliferation in peripheral blood mononuclear cells of A. fumigatus-allergic subjects who showed specific IgE responses and reacted in skin tests to MnSOD. These observations provide evidence for autoreactivity to the human MnSOD in allergic persons sensitized to an environmental allergen from A. fumigatus who share a high degree of sequence homology to the corresponding human enzyme.
Similar articles
- Humoral and cell-mediated autoimmune reactions to human acidic ribosomal P2 protein in individuals sensitized to Aspergillus fumigatus P2 protein.
Mayer C, Appenzeller U, Seelbach H, Achatz G, Oberkofler H, Breitenbach M, Blaser K, Crameri R. Mayer C, et al. J Exp Med. 1999 May 3;189(9):1507-12. doi: 10.1084/jem.189.9.1507. J Exp Med. 1999. PMID: 10224291 Free PMC article. - Identification of a Hevea brasiliensis latex manganese superoxide dismutase (Hev b 10) as a cross-reactive allergen.
Wagner S, Sowka S, Mayer C, Crameri R, Focke M, Kurup VP, Scheiner O, Breiteneder H. Wagner S, et al. Int Arch Allergy Immunol. 2001 Jun;125(2):120-7. doi: 10.1159/000053805. Int Arch Allergy Immunol. 2001. PMID: 11435728 - Molecular and immunological characterization of Asp f 34, a novel major cell wall allergen of Aspergillus fumigatus.
Glaser AG, Kirsch AI, Zeller S, Menz G, Rhyner C, Crameri R. Glaser AG, et al. Allergy. 2009 Aug;64(8):1144-51. doi: 10.1111/j.1398-9995.2009.02029.x. Epub 2009 Mar 23. Allergy. 2009. PMID: 19416144 - Recombinant Aspergillus fumigatus allergens: from the nucleotide sequences to clinical applications.
Crameri R. Crameri R. Int Arch Allergy Immunol. 1998 Feb;115(2):99-114. doi: 10.1159/000023889. Int Arch Allergy Immunol. 1998. PMID: 9482698 Review. - Serological diagnosis of allergic bronchopulmonary mycosis: Progress and challenges.
Fukutomi Y, Tanimoto H, Yasueda H, Taniguchi M. Fukutomi Y, et al. Allergol Int. 2016 Jan;65(1):30-6. doi: 10.1016/j.alit.2015.08.004. Epub 2015 Sep 26. Allergol Int. 2016. PMID: 26740298 Review.
Cited by
- Albumins represent highly cross-reactive animal allergens.
Liu Z, Trifonova D, Tulaeva I, Riabova K, Karsonova A, Kozlov E, Elisyutina O, Khaitov M, Focke-Tejkl M, Chen TH, Karaulov A, Valenta R. Liu Z, et al. Front Immunol. 2023 Oct 20;14:1241518. doi: 10.3389/fimmu.2023.1241518. eCollection 2023. Front Immunol. 2023. PMID: 37928538 Free PMC article. Review. - Molecular Characterization and the Essential Biological Function of the Metal Chaperone Protein MtmA in Aspergillus fumigatus.
Zhai P, Ma Y, Xu H, Lu L. Zhai P, et al. Appl Environ Microbiol. 2022 May 10;88(9):e0018222. doi: 10.1128/aem.00182-22. Epub 2022 Apr 18. Appl Environ Microbiol. 2022. PMID: 35435716 Free PMC article. - Changes in the Sensitization Pattern to Alternaria alternata Allergens in Patients Treated with Alt a 1 Immunotherapy.
Rodríguez D, Tabar AI, Castillo M, Martínez-Gomariz M, Dobski IC, Palacios R. Rodríguez D, et al. J Fungi (Basel). 2021 Nov 16;7(11):974. doi: 10.3390/jof7110974. J Fungi (Basel). 2021. PMID: 34829261 Free PMC article. - The Copper Chaperone CcsA, Coupled with Superoxide Dismutase SodA, Mediates the Oxidative Stress Response in Aspergillus fumigatus.
Du W, Zhai P, Liu S, Zhang Y, Lu L. Du W, et al. Appl Environ Microbiol. 2021 Aug 11;87(17):e0101321. doi: 10.1128/AEM.01013-21. Epub 2021 Aug 11. Appl Environ Microbiol. 2021. PMID: 34160279 Free PMC article. - Molecular characterization of a fungal cyclophilin allergen Rhi o 2 and elucidation of antigenic determinants responsible for IgE-cross-reactivity.
Sircar G, Bhowmik M, Sarkar RK, Najafi N, Dasgupta A, Focke-Tejkl M, Flicker S, Mittermann I, Valenta R, Bhattacharya K, Gupta Bhattacharya S. Sircar G, et al. J Biol Chem. 2020 Feb 28;295(9):2736-2748. doi: 10.1074/jbc.RA119.011659. Epub 2019 Dec 27. J Biol Chem. 2020. PMID: 31882546 Free PMC article.
References
- Gene. 1993 Dec 27;137(1):69-75 - PubMed
- Am J Respir Crit Care Med. 1995 Aug;152(2):634-9 - PubMed
- J Allergy Clin Immunol. 1994 Jan;93(1 Pt 1):1-11 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
- Proc Natl Acad Sci U S A. 1980 Feb;77(2):1159-63 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical