Natural transformation in river epilithon - PubMed (original) (raw)
Natural transformation in river epilithon
H G Williams et al. Appl Environ Microbiol. 1996 Aug.
Abstract
Natural transformation was demonstrated in unenclosed experiments incubated in river epilithon. Strains of Acinetobacter calcoaceticus were transformed to prototrophy by either free DNA (lysates) or live donor cells. The sources of transforming DNA and recipient culture were immobilized on filters, secured to stones, and incubated midstream in the river. The transfer frequency generally increased with temperature. No transfer was detected in the river Taff below 10 degrees C. The age of the recipient culture affected the transformation frequencies in situ but did not significantly affect the transfer frequency on laboratory media. Transformation of recipient cultures which had been incorporated into the natural epilithic biofilm and transformation of the plasmid pQM17 in situ were also demonstrated. This study provides the first direct evidence of natural transformation in situ of bacteria incorporated into an indigenous community.
Similar articles
- Occurrence, transfer and mobilization in epilithic strains of Acinetobacter of mercury-resistance plasmids capable of transformation.
Rochelle PA, Day MJ, Fry JC. Rochelle PA, et al. J Gen Microbiol. 1988 Nov;134(11):2933-41. doi: 10.1099/00221287-134-11-2933. J Gen Microbiol. 1988. PMID: 3254940 - Plasmid transformation of naturally competent Acinetobacter calcoaceticus in non-sterile soil extract and groundwater.
Lorenz MG, Reipschläger K, Wackernagel W. Lorenz MG, et al. Arch Microbiol. 1992;157(4):355-60. doi: 10.1007/BF00248681. Arch Microbiol. 1992. PMID: 1590708 - Transfer and occurrence of large mercury resistance plasmids in river epilithon.
Bale MJ, Fry JC, Day MJ. Bale MJ, et al. Appl Environ Microbiol. 1988 Apr;54(4):972-8. doi: 10.1128/aem.54.4.972-978.1988. Appl Environ Microbiol. 1988. PMID: 16347617 Free PMC article. - Uptake and processing of DNA by Acinetobacter calcoaceticus--a review.
Palmen R, Hellingwerf KJ. Palmen R, et al. Gene. 1997 Jun 11;192(1):179-90. doi: 10.1016/s0378-1119(97)00042-5. Gene. 1997. PMID: 9224889 Review. - Bacterial gene transfer by natural genetic transformation in the environment.
Lorenz MG, Wackernagel W. Lorenz MG, et al. Microbiol Rev. 1994 Sep;58(3):563-602. doi: 10.1128/mr.58.3.563-602.1994. Microbiol Rev. 1994. PMID: 7968924 Free PMC article. Review.
Cited by
- Extracellular DNA (eDNA): Neglected and Potential Sources of Antibiotic Resistant Genes (ARGs) in the Aquatic Environments.
Sivalingam P, Poté J, Prabakar K. Sivalingam P, et al. Pathogens. 2020 Oct 23;9(11):874. doi: 10.3390/pathogens9110874. Pathogens. 2020. PMID: 33114079 Free PMC article. Review. - Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection.
Roy S, Chowdhury G, Mukhopadhyay AK, Dutta S, Basu S. Roy S, et al. Front Med (Lausanne). 2022 Mar 24;9:793615. doi: 10.3389/fmed.2022.793615. eCollection 2022. Front Med (Lausanne). 2022. PMID: 35402433 Free PMC article. Review. - Birth, death, and diversification of mobile promoters in prokaryotes.
van Passel MW, Nijveen H, Wahl LM. van Passel MW, et al. Genetics. 2014 May;197(1):291-9. doi: 10.1534/genetics.114.162883. Epub 2014 Feb 27. Genetics. 2014. PMID: 24578351 Free PMC article. - Prevalence of Antibiotic-Resistant Escherichia coli in Drinking Water Sources in Hangzhou City.
Chen Z, Yu D, He S, Ye H, Zhang L, Wen Y, Zhang W, Shu L, Chen S. Chen Z, et al. Front Microbiol. 2017 Jun 16;8:1133. doi: 10.3389/fmicb.2017.01133. eCollection 2017. Front Microbiol. 2017. PMID: 28670309 Free PMC article. - Natural genetic transformation in monoculture Acinetobacter sp. strain BD413 biofilms.
Hendrickx L, Hausner M, Wuertz S. Hendrickx L, et al. Appl Environ Microbiol. 2003 Mar;69(3):1721-7. doi: 10.1128/AEM.69.3.1721-1727.2003. Appl Environ Microbiol. 2003. PMID: 12620864 Free PMC article.
References
- J Bacteriol. 1982 Dec;152(3):1066-70 - PubMed
- J Bacteriol. 1972 Nov;112(2):917-31 - PubMed
- Appl Environ Microbiol. 1993 May;59(5):1662-7 - PubMed
- Appl Environ Microbiol. 1990 Jun;56(6):1818-24 - PubMed
- Appl Environ Microbiol. 1988 Nov;54(11):2756-8 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources