Role of the C terminus of the interleukin 8 receptor in signal transduction and internalization - PubMed (original) (raw)
. 1996 Aug 9;271(32):19186-90.
doi: 10.1074/jbc.271.32.19186.
Affiliations
- PMID: 8702597
- DOI: 10.1074/jbc.271.32.19186
Free article
Role of the C terminus of the interleukin 8 receptor in signal transduction and internalization
G N Prado et al. J Biol Chem. 1996.
Free article
Abstract
Interleukin 8 (IL-8) is a potent neutrophil chemoattractant and activator. Two IL-8 receptor subtypes, A and B, are expressed in neutrophils. In this work, we analyzed the role of the C terminus domain of the IL-8 receptor on the signal transduction and receptor internalization mechanisms. The IL-8 receptor A was tagged with an epitope corresponding to the monoclonal antibody 1D4 to monitor the localization of the IL-8 receptor. We demonstrated IL-8-dependent receptor internalization by monitoring the density of surface 125I-labeled IL-8 binding sites and by immunofluorescence microscopy. Truncation of the last 27 amino acids of the IL-8 receptor A severely impaired the IL-8-induced internalization of the receptor. Of importance was the observation that binding of IL-8 to receptors A and B triggered a dramatically faster rate of internalization of receptor B than receptor A, suggesting that the heterologous C termini among receptor subtypes modulate the rate of internalization of IL-8 receptors. However, substitution of the C terminus of the receptor subtype A for the C terminus of receptor B reduced the internalization rate of receptor A. Furthermore, we found that the rate of internalization of IL-8 receptor B triggered by IL-8 was faster than the one induced by the IL-8-related peptide, melanoma growth stimulatory activity. Studies with human neutrophils pretreated with 100 nM IL-8 for 5 min revealed a positive and a negative calcium response mediated by receptors A and B, respectively. In contrast, neutrophils pretreated with melanoma growth stimulatory activity showed positive calcium responses to both receptors A and B. These data suggest that the neutrophil responses mediated by IL-8 are modulated by the rate of internalization of receptors.
Similar articles
- Reconstitution of chemokine-induced actin polymerization in undifferentiated human leukemia cells (HL-60) by heterologous expression of interleukin-8 receptors.
Norgauer J, Metzner B, Czech W, Schraufstatter I. Norgauer J, et al. Inflamm Res. 1996 Mar;45(3):127-31. doi: 10.1007/BF02265165. Inflamm Res. 1996. PMID: 8689391 - Neutrophil receptors for interleukin-8 and related CXC chemokines.
Murphy PM. Murphy PM. Semin Hematol. 1997 Oct;34(4):311-8. Semin Hematol. 1997. PMID: 9347581 Review.
Cited by
- Emergence of dysfunctional neutrophils with a defect in arginase-1 release in severe COVID-19.
Dwivedi A, Ui Mhaonaigh A, Carroll M, Khosravi B, Batten I, Ballantine RS, Hendricken Phelan S, O'Doherty L, George AM, Sui J, Hawerkamp HC, Fallon PG, Noppe E, Mason S, Conlon N, Ni Cheallaigh C, Finlay CM, Little MA, Bioresource OBOTSJATTARS. Dwivedi A, et al. JCI Insight. 2024 Sep 10;9(17):e171659. doi: 10.1172/jci.insight.171659. JCI Insight. 2024. PMID: 39253969 Free PMC article. - Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases.
Ha H, Debnath B, Neamati N. Ha H, et al. Theranostics. 2017 Apr 7;7(6):1543-1588. doi: 10.7150/thno.15625. eCollection 2017. Theranostics. 2017. PMID: 28529637 Free PMC article. Review. - The chemokine receptors CXCR1 and CXCR2 couple to distinct G protein-coupled receptor kinases to mediate and regulate leukocyte functions.
Raghuwanshi SK, Su Y, Singh V, Haynes K, Richmond A, Richardson RM. Raghuwanshi SK, et al. J Immunol. 2012 Sep 15;189(6):2824-32. doi: 10.4049/jimmunol.1201114. Epub 2012 Aug 6. J Immunol. 2012. PMID: 22869904 Free PMC article. - Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2.
Aziz M, Matsuda A, Yang WL, Jacob A, Wang P. Aziz M, et al. J Immunol. 2012 Jul 1;189(1):393-402. doi: 10.4049/jimmunol.1200262. Epub 2012 May 25. J Immunol. 2012. PMID: 22634615 Free PMC article. - CXCL5 regulates chemokine scavenging and pulmonary host defense to bacterial infection.
Mei J, Liu Y, Dai N, Favara M, Greene T, Jeyaseelan S, Poncz M, Lee JS, Worthen GS. Mei J, et al. Immunity. 2010 Jul 23;33(1):106-17. doi: 10.1016/j.immuni.2010.07.009. Immunity. 2010. PMID: 20643340 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources