Accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in normal and neoplastic human endometrial epithelial cells - PubMed (original) (raw)
. 1996 Jul 25;224(3):819-24.
doi: 10.1006/bbrc.1996.1106.
Affiliations
- PMID: 8713129
- DOI: 10.1006/bbrc.1996.1106
Accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in normal and neoplastic human endometrial epithelial cells
M T Wyss-Desserich et al. Biochem Biophys Res Commun. 1996.
Abstract
The aim of this study was to evaluate 5-Aminolevulinic acid (ALA)-induced fluorescence of normal and neoplastic endometrial epithelial cells for diagnosis and photodynamic treatment. Fluorescence of ALA-induced PpIX in vitro was measured by flow cytometry in two different human endometrial adenocarcinoma cell lines and in normal cells cultivated from fresh endometrial tissue of three premenopausal patients. The cells were analysed after incubation with different concentrations of ALA during 3, 6, or 24 hours. Both tumor cell lines showed a statistically significant higher fluorescence of PpIX than normal epithelial cells after incubation with 1 mg ALA per ml medium during 24 hours. The well-differentiated cancer cells produced significantly more PpIX than the poorly differentiated cancer cells. Relative PpIX intensity of the two cancer cell lines correlated with cell proliferation rate as measured by the doubling times of the cells. Higher accumulation of Pp IX in neoplastic endometrium compared to normal endometrial epithelial cells may provide targeted biopsies and selective photodynamic destruction of neoplastic micro-lesions.
Similar articles
- Effect of 5-aminolevulinic acid (ALA) doses and oestrogen/progesterone on protoporphyrin IX (ppIX) accumulation in human endometrial epithelial cells.
Butowska W, Warchoł W, Nowak-Markwitz E, Wołuń-Cholewa M. Butowska W, et al. Rocz Akad Med Bialymst. 2004;49 Suppl 1:123-5. Rocz Akad Med Bialymst. 2004. PMID: 15638395 - Photodynamic diagnosis (PDD) using 5-aminolevulinic acid-supplemented cultures of human endometrial epithelial cells.
Wołuń-Cholewa M, Warchoł W, Butowska W, Nowak-Markwitz E, Fischer N. Wołuń-Cholewa M, et al. Adv Med Sci. 2006;51:184-7. Adv Med Sci. 2006. PMID: 17357305 - Influence of the menstrual cycle on aminolevulinic acid induced protoporphyrin IX fluorescence in the endometrium: in vivo study.
Degen A, Gabrecht T, Wagnières G, Caduff R, Imthurn B, Wyss P. Degen A, et al. Lasers Surg Med. 2005 Mar;36(3):234-7. doi: 10.1002/lsm.20139. Lasers Surg Med. 2005. PMID: 15704091 - Effect of 5-aminolevulinic acid dose and estrogen on protoporphyrin IX concentrations in the rat uterus.
Roy BN, Van Vugt DA, Weagle GE, Pottier RH, Reid RL. Roy BN, et al. J Soc Gynecol Investig. 1997 Jan-Feb;4(1):40-6. doi: 10.1016/S1071-5576(96)00062-7. J Soc Gynecol Investig. 1997. PMID: 9051633 - Photodynamic therapy (PDT) and photodiagnosis (PD) using endogenous photosensitization induced by 5-aminolevulinic acid (ALA): current clinical and development status.
Marcus SL, Sobel RS, Golub AL, Carroll RL, Lundahl S, Shulman DG. Marcus SL, et al. J Clin Laser Med Surg. 1996 Apr;14(2):59-66. doi: 10.1089/clm.1996.14.59. J Clin Laser Med Surg. 1996. PMID: 9484077 Review.
Cited by
- Refining the Intraoperative Identification of Suspected High-Grade Glioma Using a Surgical Fluorescence Biomarker: GALA BIDD Study Report.
Watts C, Dayimu A, Matys T, Ashkan K, Price S, Jenkinson MD, Doughton G, Mather C, Young G, Qian W, Kurian KM. Watts C, et al. J Pers Med. 2023 Mar 13;13(3):514. doi: 10.3390/jpm13030514. J Pers Med. 2023. PMID: 36983696 Free PMC article. - Delta-Aminolevulinic Acid-Mediated Photodiagnoses in Surgical Oncology: A Historical Review of Clinical Trials.
Georges JF, Valeri A, Wang H, Brooking A, Kakareka M, Cho SS, Al-Atrache Z, Bamimore M, Osman H, Ifrach J, Yu S, Li C, Appelt D, Lee JYK, Nakaji P, Brill K, Yocom S. Georges JF, et al. Front Surg. 2019 Sep 4;6:45. doi: 10.3389/fsurg.2019.00045. eCollection 2019. Front Surg. 2019. PMID: 31555659 Free PMC article. Review. - Unknown biological effects of L-glucose, ALA, and PUFA.
Yamada K, Sato D, Nakamura T, Amano H, Morimoto Y. Yamada K, et al. J Physiol Sci. 2017 Sep;67(5):539-548. doi: 10.1007/s12576-017-0544-x. Epub 2017 May 30. J Physiol Sci. 2017. PMID: 28560575 Free PMC article. Review. - In vitro comparison of hypericin and 5-aminolevulinic acid-derived protoporphyrin IX for photodynamic inactivation of medulloblastoma cells.
Ritz R, Scheidle C, Noell S, Roser F, Schenk M, Dietz K, Strauss WS. Ritz R, et al. PLoS One. 2012;7(12):e51974. doi: 10.1371/journal.pone.0051974. Epub 2012 Dec 14. PLoS One. 2012. PMID: 23251668 Free PMC article. - Imaging and photodynamic therapy: mechanisms, monitoring, and optimization.
Celli JP, Spring BQ, Rizvi I, Evans CL, Samkoe KS, Verma S, Pogue BW, Hasan T. Celli JP, et al. Chem Rev. 2010 May 12;110(5):2795-838. doi: 10.1021/cr900300p. Chem Rev. 2010. PMID: 20353192 Free PMC article. Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous