Bone morphogenetic protein-2 but not bone morphogenetic protein-4 and -6 stimulates chemotactic migration of human osteoblasts, human marrow osteoblasts, and U2-OS cells - PubMed (original) (raw)
Comparative Study
Bone morphogenetic protein-2 but not bone morphogenetic protein-4 and -6 stimulates chemotactic migration of human osteoblasts, human marrow osteoblasts, and U2-OS cells
M LInd et al. Bone. 1996 Jan.
Abstract
Bone morphogenetic proteins (BMPs) have important functions for the differentiation of bone cells, but the exact role for bone remodeling and bone healing still needs to be defined. Migration of bone forming cells is an important physiological event both during bone healing and bone remodeling. The chemotatic properties of the bone morphogenetic protein family of growth factors have not been investigated. In this study the chemotactic effects of the bone morphogenetic proteins BMP-2, -4, and -6 have been quantitated toward human osteoblasts, human marrow stromal osteoblasts, and U2-OS human osteosarcoma cells. BMP-2 stimulated the migration of human stromal osteoblasts, human osteoblasts, and U2-OS cells with bell-shaped response curves in a dose-dependent manner with a 300% increase in cell migration at 1.0 ng/mL for human stromal osteoblasts and a 170-180% increase for human osteoblasts and U2-OS cells. At higher concentrations, migration decreased to background levels. BMP-4 and -6 did not show any effect on cellular migration. This study shows that BMP-2 can stimulate in vitro migration of human osteoblasts and human osteosarcoma cells. BMP-2 might play a role in the chemotactic recruitment of especially undifferentiated osteoblasts during bone remodeling and bone healing.
Similar articles
- BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells.
Fiedler J, Röderer G, Günther KP, Brenner RE. Fiedler J, et al. J Cell Biochem. 2002;87(3):305-12. doi: 10.1002/jcb.10309. J Cell Biochem. 2002. PMID: 12397612 - Growth factor stimulation of bone healing. Effects on osteoblasts, osteomies, and implants fixation.
Lind M. Lind M. Acta Orthop Scand Suppl. 1998 Oct;283:2-37. Acta Orthop Scand Suppl. 1998. PMID: 9856074 - Effects of BMP-2, BMP-4, and BMP-6 on osteoblastic differentiation of bone marrow-derived stromal cell lines, ST2 and MC3T3-G2/PA6.
Yamaguchi A, Ishizuya T, Kintou N, Wada Y, Katagiri T, Wozney JM, Rosen V, Yoshiki S. Yamaguchi A, et al. Biochem Biophys Res Commun. 1996 Mar 18;220(2):366-71. doi: 10.1006/bbrc.1996.0411. Biochem Biophys Res Commun. 1996. PMID: 8645311 - Bone morphogenetic proteins 5 and 6 stimulate osteoclast generation.
Wutzl A, Brozek W, Lernbass I, Rauner M, Hofbauer G, Schopper C, Watzinger F, Peterlik M, Pietschmann P. Wutzl A, et al. J Biomed Mater Res A. 2006 Apr;77(1):75-83. doi: 10.1002/jbm.a.30615. J Biomed Mater Res A. 2006. PMID: 16355411 - Recombinant bone morphogenetic proteins: novel substances for enhancing bone healing.
Kirker-Head CA. Kirker-Head CA. Vet Surg. 1995 Sep-Oct;24(5):408-19. doi: 10.1111/j.1532-950x.1995.tb01352.x. Vet Surg. 1995. PMID: 8585149 Review.
Cited by
- Computational model of mesenchymal migration in 3D under chemotaxis.
Ribeiro FO, Gómez-Benito MJ, Folgado J, Fernandes PR, García-Aznar JM. Ribeiro FO, et al. Comput Methods Biomech Biomed Engin. 2017 Jan;20(1):59-74. doi: 10.1080/10255842.2016.1198784. Epub 2016 Jun 23. Comput Methods Biomech Biomed Engin. 2017. PMID: 27336322 Free PMC article. - Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts.
Yun HM, Kim E, Kwon YJ, Park KR. Yun HM, et al. Pharmaceutics. 2024 Apr 1;16(4):485. doi: 10.3390/pharmaceutics16040485. Pharmaceutics. 2024. PMID: 38675146 Free PMC article. - Enhanced in vivo retention of low dose BMP-2 via heparin microparticle delivery does not accelerate bone healing in a critically sized femoral defect.
Hettiaratchi MH, Rouse T, Chou C, Krishnan L, Stevens HY, Li MA, McDevitt TC, Guldberg RE. Hettiaratchi MH, et al. Acta Biomater. 2017 Sep 1;59:21-32. doi: 10.1016/j.actbio.2017.06.028. Epub 2017 Jun 20. Acta Biomater. 2017. PMID: 28645809 Free PMC article. - A miR-125b binding site polymorphism in bone morphogenetic protein membrane receptor type IB gene and prostate cancer risk in China.
Feng N, Xu B, Tao J, Li P, Cheng G, Min Z, Mi Y, Wang M, Tong N, Tang J, Zhang Z, Wu H, Zhang W, Wang Z, Hua L. Feng N, et al. Mol Biol Rep. 2012 Jan;39(1):369-73. doi: 10.1007/s11033-011-0747-9. Epub 2011 May 10. Mol Biol Rep. 2012. PMID: 21556765 - Bone morphogenetic protein 6 in skeletal metastases from prostate cancer and other common human malignancies.
Autzen P, Robson CN, Bjartell A, Malcolm AJ, Johnson MI, Neal DE, Hamdy FC. Autzen P, et al. Br J Cancer. 1998 Nov;78(9):1219-23. doi: 10.1038/bjc.1998.658. Br J Cancer. 1998. PMID: 9820184 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources