Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast - PubMed (original) (raw)

Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast

K A Becherer et al. Mol Biol Cell. 1996 Apr.

Free PMC article

Abstract

pep12/vps6 mutants of Saccharomyces cerevisiae are defective in delivery of soluble vacuolar hydrolases to the vacuole. Morphological analysis by electron microscopy revealed that pep12 cells accumulate 40- to 50-nm vesicles. Furthermore, pep12 cells have enlarged vacuoles characteristic of class D pep/vps mutants. PEP12 encodes a protein of 288 amino acids that has a C-terminal hydrophobic region and shares significant sequence similarity with members of the syntaxin protein family. These proteins appear to participate in the docking and fusion of intracellular transport vesicles. Pep12p is the first member of the syntaxin family to be implicated in transport between the Golgi and the vacuole/lysosome. Pep12p-specific polyclonal antisera detected a 35-kDa protein that fractionated as an integral membrane protein. Subcellular fractionation experiments revealed that Pep12p was associated with membrane fractions of two different densities; the major pool (approximately 90%) of pep12p may associate with the endosome, while a minor pool (approximately 10%) cofractionated with the late Golgi marker Kex2p. These observations suggest that Pep12p may mediate the docking of Golgi-derived transport vesicles at the endosome.

PubMed Disclaimer

Similar articles

• Pth1/Vam3p is the syntaxin homolog at the vacuolar membrane of Saccharomyces cerevisiae required for the delivery of vacuolar hydrolases.
  Srivastava A, Jones EW. Srivastava A, et al. Genetics. 1998 Jan;148(1):85-98. doi: 10.1093/genetics/148.1.85. Genetics. 1998. PMID: 9475723 Free PMC article.
• A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.
  Burd CG, Peterson M, Cowles CR, Emr SD. Burd CG, et al. Mol Biol Cell. 1997 Jun;8(6):1089-104. doi: 10.1091/mbc.8.6.1089. Mol Biol Cell. 1997. PMID: 9201718 Free PMC article.
• Endosome to Golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products.
  Seaman MN, Marcusson EG, Cereghino JL, Emr SD. Seaman MN, et al. J Cell Biol. 1997 Apr 7;137(1):79-92. doi: 10.1083/jcb.137.1.79. J Cell Biol. 1997. PMID: 9105038 Free PMC article.
• Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins.
  Stack JH, Horazdovsky B, Emr SD. Stack JH, et al. Annu Rev Cell Dev Biol. 1995;11:1-33. doi: 10.1146/annurev.cb.11.110195.000245. Annu Rev Cell Dev Biol. 1995. PMID: 8689553 Review.
• The fungal vacuole: composition, function, and biogenesis.
  Klionsky DJ, Herman PK, Emr SD. Klionsky DJ, et al. Microbiol Rev. 1990 Sep;54(3):266-92. doi: 10.1128/mr.54.3.266-292.1990. Microbiol Rev. 1990. PMID: 2215422 Free PMC article. Review.

Cited by

• Golgi membrane-associated degradation pathway in yeast and mammals.
  Yamaguchi H, Arakawa S, Kanaseki T, Miyatsuka T, Fujitani Y, Watada H, Tsujimoto Y, Shimizu S. Yamaguchi H, et al. EMBO J. 2016 Sep 15;35(18):1991-2007. doi: 10.15252/embj.201593191. Epub 2016 Aug 10. EMBO J. 2016. PMID: 27511903 Free PMC article.
• Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion.
  Mullock BM, Smith CW, Ihrke G, Bright NA, Lindsay M, Parkinson EJ, Brooks DA, Parton RG, James DE, Luzio JP, Piper RC. Mullock BM, et al. Mol Biol Cell. 2000 Sep;11(9):3137-53. doi: 10.1091/mbc.11.9.3137. Mol Biol Cell. 2000. PMID: 10982406 Free PMC article.
• A selective transport route from Golgi to late endosomes that requires the yeast GGA proteins.
  Black MW, Pelham HR. Black MW, et al. J Cell Biol. 2000 Oct 30;151(3):587-600. doi: 10.1083/jcb.151.3.587. J Cell Biol. 2000. PMID: 11062260 Free PMC article.
• Autophagy in the eukaryotic cell.
  Reggiori F, Klionsky DJ. Reggiori F, et al. Eukaryot Cell. 2002 Feb;1(1):11-21. doi: 10.1128/EC.01.1.11-21.2002. Eukaryot Cell. 2002. PMID: 12455967 Free PMC article. Review. No abstract available.
• Vam7p, a SNAP-25-like molecule, and Vam3p, a syntaxin homolog, function together in yeast vacuolar protein trafficking.
  Sato TK, Darsow T, Emr SD. Sato TK, et al. Mol Cell Biol. 1998 Sep;18(9):5308-19. doi: 10.1128/MCB.18.9.5308. Mol Cell Biol. 1998. PMID: 9710615 Free PMC article.

References

1. 1. J Mol Biol. 1975 Oct 25;98(2):293-304 - PubMed
2. 1. J Biol Chem. 1992 Jun 15;267(17):12106-15 - PubMed
3. 1. Gene. 1984 Feb;27(2):183-91 - PubMed
4. 1. J Biol Chem. 1995 Jul 14;270(28):16955-61 - PubMed
5. 1. Nature. 1994 Nov 3;372(6501):55-63 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database

• Miscellaneous

  • NCI CPTAC Assay Portal