CD8 T-cell recognition of macrophages and hepatocytes results in immunity to Listeria monocytogenes - PubMed (original) (raw)

CD8 T-cell recognition of macrophages and hepatocytes results in immunity to Listeria monocytogenes

J T Harty et al. Infect Immun. 1996 Sep.

Abstract

CD8 T cells are effective mediators of specific immunity to infection by Listeria monocytogenes, a bacterial pathogen that initially infects macrophages in the spleen and liver and subsequently spreads to hepatocytes and unidentified parenchymal cells in the spleen. To identify the in vivo target cells of L. monocytogenes-immune CD8 T cells, adoptive transfer assays were performed with bone marrow chimeric or transgenic host mice which had been manipulated to alter the major histocompatibility complex molecules expressed on macrophages or hepatocytes. L. monocytogenes-immune CD8 T cells mediate significant immunity in BDF1-->beta 2 M-/- chimeras, comparable to that seen in unmanipulated BDF1 recipients. L. monocytogenes-immune CD8 T cells also mediate significant antilisterial immunity in parent-->F1 chimeras when the CD8 T cells are syngeneic with the bone marrow donor. These data demonstrate that bone marrow-derived macrophages are major targets for L. monocytogenes-immune CD8 T cells in adoptive transfer assays. Interestingly, significant immunity was observed in parent-->F1 chimeras when the L. monocytogenes-immune CD8 T cells were not syngeneic with the bone marrow donor, suggesting that recognition of Listeria-infected non-bone-marrow-derived cells such as hepatocytes may also occur in vivo. Consistent with this possibility, H-2Kb-restricted CD8 T cells specific for the listeriolysin O molecule mediate significant immunity in the liver, but not the spleen, in transgenic mice expressing H-2Kb only on hepatocytes. In addition, Listeria-specific CD8 T cells lyse Listeria-infected hepatocyte-like cells in vitro. Thus, Listeria-infected hepatocytes can be recognized by CD8 T cells in vivo and in vitro.

PubMed Disclaimer

Similar articles

• Listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class Ib-restricted antilisterial cytotoxic T lymphocytes.
  Bouwer HG, Bai A, Forman J, Gregory SH, Wing EJ, Barry RA, Hinrichs DJ. Bouwer HG, et al. Infect Immun. 1998 Jun;66(6):2814-7. doi: 10.1128/IAI.66.6.2814-2817.1998. Infect Immun. 1998. PMID: 9596753 Free PMC article.
• Class Ia MHC-deficient BALB/c mice generate CD8+ T cell-mediated protective immunity against Listeria monocytogenes infection.
  D'Orazio SE, Halme DG, Ploegh HL, Starnbach MN. D'Orazio SE, et al. J Immunol. 2003 Jul 1;171(1):291-8. doi: 10.4049/jimmunol.171.1.291. J Immunol. 2003. PMID: 12817010
• CD8+ T-cell-mediated response to Listeria monocytogenes taken up in the liver and replicating within hepatocytes.
  Gregory SH, Liu CC. Gregory SH, et al. Immunol Rev. 2000 Apr;174:112-22. doi: 10.1034/j.1600-0528.2002.017405.x. Immunol Rev. 2000. PMID: 10807511 Review.
• Acquired immunity to an intracellular pathogen: immunologic recognition of L. monocytogenes-infected cells.
  Bouwer HG, Barry RA, Hinrichs DJ. Bouwer HG, et al. Immunol Rev. 1997 Aug;158:137-46. doi: 10.1111/j.1600-065x.1997.tb01000.x. Immunol Rev. 1997. PMID: 9314082 Review.

Cited by

• Context-dependent effects of SOCS3 in angiotensin II-induced vascular dysfunction and hypertension in mice: mechanisms and role of bone marrow-derived cells.
  Li Y, Kinzenbaw DA, Modrick ML, Pewe LL, Faraci FM. Li Y, et al. Am J Physiol Heart Circ Physiol. 2016 Jul 1;311(1):H146-56. doi: 10.1152/ajpheart.00204.2016. Epub 2016 Apr 22. Am J Physiol Heart Circ Physiol. 2016. PMID: 27106041 Free PMC article.
• Modes of Antigen Presentation by Lymph Node Stromal Cells and Their Immunological Implications.
  Hirosue S, Dubrot J. Hirosue S, et al. Front Immunol. 2015 Sep 8;6:446. doi: 10.3389/fimmu.2015.00446. eCollection 2015. Front Immunol. 2015. PMID: 26441957 Free PMC article. Review.
• Fas (CD95)-dependent cell-mediated immunity to Listeria monocytogenes.
  Jensen ER, Glass AA, Clark WR, Wing EJ, Miller JF, Gregory SH. Jensen ER, et al. Infect Immun. 1998 Sep;66(9):4143-50. doi: 10.1128/IAI.66.9.4143-4150.1998. Infect Immun. 1998. PMID: 9712760 Free PMC article.
• Recovery from mouse hepatitis virus infection depends on recruitment of CD8(+) cells rather than activation of intrahepatic CD4(+)alphabeta(-)TCR(inter) or NK-T cells.
  Lamontagne L, Lusignan S, Page C. Lamontagne L, et al. Clin Immunol. 2001 Dec;101(3):345-56. doi: 10.1006/clim.2001.5131. Clin Immunol. 2001. PMID: 11726227 Free PMC article.
• Adaptive immunity against Listeria monocytogenes in the absence of type I tumor necrosis factor receptor p55.
  White DW, Badovinac VP, Fan X, Harty JT. White DW, et al. Infect Immun. 2000 Aug;68(8):4470-6. doi: 10.1128/IAI.68.8.4470-4476.2000. Infect Immun. 2000. PMID: 10899844 Free PMC article.

References

1. 1. Science. 1990 Jun 8;248(4960):1227-30 - PubMed
2. 1. J Exp Med. 1962 Sep 1;116:381-406 - PubMed
3. 1. J Immunol. 1990 Dec 1;145(11):3540-6 - PubMed
4. 1. J Pediatr Gastroenterol Nutr. 1990 Nov;11(4):525-33 - PubMed
5. 1. Immunol Rev. 1991 Aug;122:47-67 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program