Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor - PubMed (original) (raw)

Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor

K Frazier et al. J Invest Dermatol. 1996 Sep.

Free article

Abstract

Connective tissue growth factor (CTGF) is a 36-to 38-kDa peptide that is selectively induced by transforming growth factor-beta (TGF-beta) in fibroblastic cell types. We compared the biologic activities of CTGF with TGF-beta on fibroblasts in culture and in animal models of fibroplasia. CTGF was active as a mitogen in monolayer cultures of normal rat kidney fibroblasts. CTGF did not stimulate anchorage-independent growth of NRK fibroblasts, however, or inhibit the growth of mink lung epithelial cells, distinguishing CTGF's growth-regulatory activities from those of TGF-beta. In NRK fibroblasts, both TGF-beta and CTGF significantly increased the transcripts encoding alpha 1 type I collagen, alpha 5 integrin, and fibronectin. Stimulation of type I collagen and fibronectin protein synthesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine. Subcutaneous injection of TGF-beta and CTGF into neonatal NIH Swiss mice resulted in a large stimulation of granulation tissue and fibrosis at the site of injection. In situ hybridization studies revealed that TGF-beta injection induced high levels of CTGF mRNA in the dermal fibroblasts at the injection site, demonstrating that TGF-beta can induce the expression of CTGF in connective tissue cells in vivo. No CTGF transcripts were detected in the epidermal cells in either control or TGF-beta-injected skin or in fibroblasts in control (saline-injected) skin. These results demonstrate that, like TGF-beta, CTGF can induce connective tissue cell proliferation and extracellular matrix synthesis.

PubMed Disclaimer

Cited by

Mast cells rescue implantation defects caused by c-kit deficiency.
Woidacki K, Popovic M, Metz M, Schumacher A, Linzke N, Teles A, Poirier F, Fest S, Jensen F, Rabinovich GA, Maurer M, Zenclussen AC. Woidacki K, et al. Cell Death Dis. 2013 Jan 17;4(1):e462. doi: 10.1038/cddis.2012.214. Cell Death Dis. 2013. PMID: 23328669 Free PMC article.
Regulation of connective tissue growth factor gene expression and fibrosis in human heart failure.
Koshman YE, Patel N, Chu M, Iyengar R, Kim T, Ersahin C, Lewis W, Heroux A, Samarel AM. Koshman YE, et al. J Card Fail. 2013 Apr;19(4):283-94. doi: 10.1016/j.cardfail.2013.01.013. Epub 2013 Mar 19. J Card Fail. 2013. PMID: 23582094 Free PMC article.
Subplate in the developing cortex of mouse and human.
Wang WZ, Hoerder-Suabedissen A, Oeschger FM, Bayatti N, Ip BK, Lindsay S, Supramaniam V, Srinivasan L, Rutherford M, Møllgård K, Clowry GJ, Molnár Z. Wang WZ, et al. J Anat. 2010 Oct;217(4):368-80. doi: 10.1111/j.1469-7580.2010.01274.x. Epub 2010 Aug 18. J Anat. 2010. PMID: 20727056 Free PMC article.
Inhibition of connective tissue growth factor overexpression in diabetic retinopathy by SERPINA3K via blocking the WNT/beta-catenin pathway.
Zhang B, Zhou KK, Ma JX. Zhang B, et al. Diabetes. 2010 Jul;59(7):1809-16. doi: 10.2337/db09-1056. Epub 2010 Mar 18. Diabetes. 2010. PMID: 20299474 Free PMC article.
Effects of mycophenolic acid on high glucose-induced expression of TGF-beta and CTGF in mesangial cells.
Lü Y, Chen J, Shao J. Lü Y, et al. J Huazhong Univ Sci Technolog Med Sci. 2006;26(3):292-3, 304. doi: 10.1007/BF02829554. J Huazhong Univ Sci Technolog Med Sci. 2006. PMID: 16961272

Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor - PubMed (original) (raw)