The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2) - PubMed (original) (raw)
. 1996 Jul 19;260(3):422-31.
doi: 10.1006/jmbi.1996.0411.
Affiliations
- PMID: 8757804
- DOI: 10.1006/jmbi.1996.0411
The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)
T L Bullock et al. J Mol Biol. 1996.
Abstract
Nuclear transport factor 2 (NTF2) facilitates protein transport into the nucleus and interacts with both the small Ras-like GTPase Ran and nucleoporin p62. We have determined the structure of bacterially expressed rat NTF2 at 1.6 angstroms resolution using X-ray crystallography. The NTF2 polypeptide chain forms an alpha + beta barrel that opens at one end to form a distinctive hydrophobic cavity and its fold is homologous to that of scytalone dehydratase. The NTF2 hydrophobic cavity is a candidate for a potential binding site for other proteins involved in nuclear import such as Ran and nucleoporin p62. In addition, the hydrophobic cavity contains a putative catalytic Asp-His pair, which raises the possibility of an unanticipated enzymatic activity of the molecule that may have implications for the molecular mechanism of nuclear protein import.
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