Functional expression of the type 1 inositol 1,4,5-trisphosphate receptor promoter-lacZ fusion genes in transgenic mice - PubMed (original) (raw)
Functional expression of the type 1 inositol 1,4,5-trisphosphate receptor promoter-lacZ fusion genes in transgenic mice
D Furutama et al. J Neurochem. 1996 May.
Abstract
Inositol 1,4,5-trisphosphate receptor (IP3R) is an inositol 1,4,5-trisphosphate (InsP3)-gated Ca2+ release channel. Type 1 IP3R (IP3R1) is the neuronal member of the IP3R family in the CNS and is predominantly expressed in cerebellar Purkinje cells. To elucidate the molecular mechanisms responsible for coupling gene expression to neuronal InsP3/Ca2+ signaling, we have studied the structure and function of the 5'-flanking region of the mouse IP3R1 gene. The cloned 5'-flanking region has several sequences sharing identity with motifs for known transcriptional regulation. We have fused 5'-flanking regions 1N from -528 to +169 and 4N from -4,187 to +169 to a beta-galactosidase gene (lacZ) as a reporter marker and have characterized their in vivo gene expression. Both 1N and 4N fusion genes functioned as a strong promoter in a neuroblastoma-glioma hybrid cell line NG108-15. Moreover, both 1N and 4N transgenic mouse lines carrying these 1N and 4N fusion genes showed characteristic patterns of beta-galactosidase activity in the CNS that are almost consistent with that of the endogenous IP3R1 protein, thereby suggesting that the 1N region from -528 to +169 contains sequence elements responsible for regulating gene expression in neurons and for specifying predominant expression in cerebellar Purkinje cells.
Similar articles
- Activation of the mouse inositol 1,4,5-trisphosphate receptor type 1 promoter by AP-2.
Ohkawa N, Konishi Y, Shimada M, Makino Y, Yoshikawa S, Mikoshiba K, Tamura T. Ohkawa N, et al. Gene. 1999 Mar 18;229(1-2):11-9. doi: 10.1016/s0378-1119(99)00048-7. Gene. 1999. PMID: 10095099 - Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor.
Matsumoto M, Nakagawa T, Inoue T, Nagata E, Tanaka K, Takano H, Minowa O, Kuno J, Sakakibara S, Yamada M, Yoneshima H, Miyawaki A, Fukuuchi Y, Furuichi T, Okano H, Mikoshiba K, Noda T. Matsumoto M, et al. Nature. 1996 Jan 11;379(6561):168-71. doi: 10.1038/379168a0. Nature. 1996. PMID: 8538767 - The isolation and characterization of the promoter of the human type 1 inositol 1,4,5-trisphosphate receptor.
Deelman LE, Jonk LJ, Henning RH. Deelman LE, et al. Gene. 1998 Jan 30;207(2):219-25. doi: 10.1016/s0378-1119(97)00630-6. Gene. 1998. PMID: 9511764 - Inositol trisphosphate receptor and Ca2+ signalling.
Mikoshiba K, Furuichi T, Miyawaki A, Yoshikawa S, Nakagawa T, Yamada N, Hamanaka Y, Fujino I, Michikawa T, Ryo Y, et al. Mikoshiba K, et al. Philos Trans R Soc Lond B Biol Sci. 1993 Jun 29;340(1293):345-9. doi: 10.1098/rstb.1993.0077. Philos Trans R Soc Lond B Biol Sci. 1993. PMID: 8103938 Review. - Intracellular calcium-release channels: regulators of cell life and death.
Marks AR. Marks AR. Am J Physiol. 1997 Feb;272(2 Pt 2):H597-605. doi: 10.1152/ajpheart.1997.272.2.H597. Am J Physiol. 1997. PMID: 9124414 Review.
Cited by
- A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-Trisphosphate Receptors.
Toprak U, Doğan C, Hegedus D. Toprak U, et al. Biomolecules. 2021 Jul 15;11(7):1031. doi: 10.3390/biom11071031. Biomolecules. 2021. PMID: 34356655 Free PMC article. Review. - Ion Channels Controlling Circadian Rhythms in Suprachiasmatic Nucleus Excitability.
Harvey JRM, Plante AE, Meredith AL. Harvey JRM, et al. Physiol Rev. 2020 Oct 1;100(4):1415-1454. doi: 10.1152/physrev.00027.2019. Epub 2020 Mar 12. Physiol Rev. 2020. PMID: 32163720 Free PMC article. Review. - Purkinje cell stripes and long-term depression at the parallel fiber-Purkinje cell synapse.
Hawkes R. Hawkes R. Front Syst Neurosci. 2014 Mar 28;8:41. doi: 10.3389/fnsys.2014.00041. eCollection 2014. Front Syst Neurosci. 2014. PMID: 24734006 Free PMC article. Review. - Clustered fine compartmentalization of the mouse embryonic cerebellar cortex and its rearrangement into the postnatal striped configuration.
Fujita H, Morita N, Furuichi T, Sugihara I. Fujita H, et al. J Neurosci. 2012 Nov 7;32(45):15688-703. doi: 10.1523/JNEUROSCI.1710-12.2012. J Neurosci. 2012. PMID: 23136409 Free PMC article. - Ca2+-dependent transcriptional control of Ca2+ homeostasis.
Naranjo JR, Mellström B. Naranjo JR, et al. J Biol Chem. 2012 Sep 14;287(38):31674-80. doi: 10.1074/jbc.R112.384982. Epub 2012 Jul 20. J Biol Chem. 2012. PMID: 22822058 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous