Recombinant adeno-associated virus-mediated high-efficiency, transient expression of the murine cationic amino acid transporter (ecotropic retroviral receptor) permits stable transduction of human HeLa cells by ecotropic retroviral vectors - PubMed (original) (raw)
Recombinant adeno-associated virus-mediated high-efficiency, transient expression of the murine cationic amino acid transporter (ecotropic retroviral receptor) permits stable transduction of human HeLa cells by ecotropic retroviral vectors
J Bertran et al. J Virol. 1996 Oct.
Abstract
Adeno-associated virus has a broad host range, is nonpathogenic, and integrates into a preferred location on chromosome 19, features that have fostered development of recombinant adeno-associated viruses (rAAV) as gene transfer vectors for therapeutic applications. We have used an rAAV to transfer and express the murine cationic amino acid transporter which functions as the ecotropic retroviral receptor, thereby rendering human cells conditionally susceptible to infection by an ecotropic retroviral vector. The proportion of human HeLa cells expressing the receptor at 60 h varied as a function of the multiplicity of infection (MOI) with the rAAV. Cells expressing the ecotropic receptor were efficiently transduced with an ecotropic retroviral vector encoding a nucleus-localized form of beta-galactosidase. Cells coexpressing the ecotropic receptor and nucleus-localized beta-galactosidase were isolated by fluorescence-activated cell sorting, and cell lines were recovered by cloning at limiting dilution. After growth in culture, all clones contained the retroviral vector genome, but fewer than 10% (3 of 47) contained the rAAV genome and continued to express the ecotropic receptor. The ecotropic receptor coding sequences in the rAAV genome were under the control of a tetracycline-modulated promoter. In the presence of tetracycline, receptor expression was low and the proportion of cells transduced by the ecotropic retroviral vector was decreased. Modulation of receptor expression was achieved with both an episomal and an integrated form of the rAAV genome. These data establish that functional gene expression from an rAAV genome can occur transiently without genome integration.
Similar articles
- Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
Qing K, Bachelot T, Mukherjee P, Wang XS, Peng L, Yoder MC, Leboulch P, Srivastava A. Qing K, et al. J Virol. 1997 Jul;71(7):5663-7. doi: 10.1128/JVI.71.7.5663-5667.1997. J Virol. 1997. PMID: 9188645 Free PMC article. - Recombinant adeno-associated virus mediates a high level of gene transfer but less efficient integration in the K562 human hematopoietic cell line.
Malik P, McQuiston SA, Yu XJ, Pepper KA, Krall WJ, Podsakoff GM, Kurtzman GJ, Kohn DB. Malik P, et al. J Virol. 1997 Mar;71(3):1776-83. doi: 10.1128/JVI.71.3.1776-1783.1997. J Virol. 1997. PMID: 9032306 Free PMC article. - Adenovirus facilitated infection of human cells with ecotropic retrovirus.
Scott-Taylor TH, Gallardo HF, Gänsbacher B, Sadelain M. Scott-Taylor TH, et al. Gene Ther. 1998 May;5(5):621-9. doi: 10.1038/sj.gt.3300633. Gene Ther. 1998. PMID: 9797866 - Recombinant adeno-associated virus transduction and integration.
Schultz BR, Chamberlain JS. Schultz BR, et al. Mol Ther. 2008 Jul;16(7):1189-99. doi: 10.1038/mt.2008.103. Epub 2008 May 20. Mol Ther. 2008. PMID: 18500252 Free PMC article. Review. - Adeno-Associated Virus (AAV) Gene Delivery: Dissecting Molecular Interactions upon Cell Entry.
Large EE, Silveria MA, Zane GM, Weerakoon O, Chapman MS. Large EE, et al. Viruses. 2021 Jul 10;13(7):1336. doi: 10.3390/v13071336. Viruses. 2021. PMID: 34372542 Free PMC article. Review.
Cited by
- Novel adeno-associated virus vector vaccine restricts replication of simian immunodeficiency virus in macaques.
Johnson PR, Schnepp BC, Connell MJ, Rohne D, Robinson S, Krivulka GR, Lord CI, Zinn R, Montefiori DC, Letvin NL, Clark KR. Johnson PR, et al. J Virol. 2005 Jan;79(2):955-65. doi: 10.1128/JVI.79.2.955-965.2005. J Virol. 2005. PMID: 15613324 Free PMC article. - Design and packaging of adeno-associated virus gene targeting vectors.
Hirata RK, Russell DW. Hirata RK, et al. J Virol. 2000 May;74(10):4612-20. doi: 10.1128/jvi.74.10.4612-4620.2000. J Virol. 2000. PMID: 10775597 Free PMC article. - Adaptation of chimeric retroviruses in vitro and in vivo: isolation of avian retroviral vectors with extended host range.
Barsov EV, Payne WS, Hughes SH. Barsov EV, et al. J Virol. 2001 Jun;75(11):4973-83. doi: 10.1128/JVI.75.11.4973-4983.2001. J Virol. 2001. PMID: 11333876 Free PMC article. - Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
Qing K, Bachelot T, Mukherjee P, Wang XS, Peng L, Yoder MC, Leboulch P, Srivastava A. Qing K, et al. J Virol. 1997 Jul;71(7):5663-7. doi: 10.1128/JVI.71.7.5663-5667.1997. J Virol. 1997. PMID: 9188645 Free PMC article. - Adeno-associated virus vector integration junctions.
Rutledge EA, Russell DW. Rutledge EA, et al. J Virol. 1997 Nov;71(11):8429-36. doi: 10.1128/JVI.71.11.8429-8436.1997. J Virol. 1997. PMID: 9343199 Free PMC article.
References
- Blood. 1996 Mar 15;87(6):2579-87 - PubMed
- Hum Gene Ther. 1995 Sep;6(9):1203-13 - PubMed
- Hum Gene Ther. 1995 Dec;6(12):1531-41 - PubMed
- J Virol. 1993 Apr;67(4):2091-6 - PubMed
- J Virol. 1993 Apr;67(4):2097-102 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources