Structural instability of a transgene locus in tobacco is associated with aneuploidy - PubMed (original) (raw)

Structural instability of a transgene locus in tobacco is associated with aneuploidy

I Papp et al. Plant J. 1996 Sep.

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Erratum in

• Plant J 1997 Jan;11(1):167

Abstract

This paper describes molecular and cytogenetic evidence for the stability of a transgene locus that is present on the triplicated chromosome in an aneuploid tobacco line. This instability was manifested in several ways in trisomics including a major chromosome rearrangement that was detectable cytogenetically, smaller scale DNA rearrangements that occurred both germinally and somatically, and methylation/epigenetic silencing. In a deletion derivative of the locus, DNA breakpoints were found in AT-rich regions. One of these regions binds to nuclear scaffolds in vitro, suggesting a possible role for aberrant topoisomerase II cleavage in destabilization of the locus. The implications of increased chromosome instability in aneuploids for plant karyotype evolution and human carcinogenesis are discussed.

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