The fate of adoptively transferred antigen-specific T cells in vivo - PubMed (original) (raw)
The fate of adoptively transferred antigen-specific T cells in vivo
L Zhang. Eur J Immunol. 1996 Sep.
Abstract
In this study, anti-major histocompatibility complex class I Ld T cell receptor (TCR)-transgenic cells were adoptively transferred into severe-combined immunodeficient (Scid) mice which express the Ld Ag on all nucleated cells, and the fate of transferred antigen-specific T cells (ASTC) was followed in vivo. It was found that, after encountering antigen (Ag) in vivo, the number of ASTC increased 10-15-fold followed by a decline in number to a value that was still above the starting value. The expansion of ASTC could be abrogated by blocking CD28 on the T cells prior to Ag stimulation. Using the technique which simultaneously stains ASTC surface markers and apoptotic cells, we have not only demonstrated directly that the ASTC that disappeared from the periphery died by activation-induced apoptosis but also studied their kinetics in vivo. The remaining ASTC moderately down-regulated both TCR and CD8 on their cell surface and were fully unresponsive when cultured with Ld+ cells even in the presence of exogenous interleukin (IL)-2 and IL-4. However, they were still susceptible to apoptosis when transferred into a secondary host that provided a new source of Ag and antigen-presenting cells. These studies indicate that peripheral T cell tolerance can be induced by multiple mechanisms in which activation-induced ASTC apoptosis plays an important role.
Similar articles
- The immunologic function of 1B2+ double negative (CD4-CD8-) T cells in the 2C transgenic mouse.
Margenthaler JA, Flye MW. Margenthaler JA, et al. J Surg Res. 2005 Jun 15;126(2):160-6. doi: 10.1016/j.jss.2005.01.018. J Surg Res. 2005. PMID: 15919414 - Regulation of apoptosis in mature alphabeta+CD4-CD8- antigen-specific suppressor T cell clones.
Khan Q, Penninger JM, Yang L, Marra LE, Kozieradzki I, Zhang L. Khan Q, et al. J Immunol. 1999 May 15;162(10):5860-7. J Immunol. 1999. PMID: 10229821 - CD28-mediated costimulation is necessary for the activation of T cell receptor-gamma delta+ T lymphocytes.
Sperling AI, Linsley PS, Barrett TA, Bluestone JA. Sperling AI, et al. J Immunol. 1993 Dec 1;151(11):6043-50. J Immunol. 1993. PMID: 8245449 - Regulatory function for murine intraepithelial lymphocytes. Two subsets of CD3+, T cell receptor-1+ intraepithelial lymphocyte T cells abrogate oral tolerance.
Fujihashi K, Taguchi T, McGhee JR, Eldridge JH, Bruce MG, Green DR, Singh B, Kiyono H. Fujihashi K, et al. J Immunol. 1990 Oct 1;145(7):2010-9. J Immunol. 1990. PMID: 1975820 - Peripheral deletion of mature CD8+ antigen-specific T cells after in vivo exposure to male antigen.
Zhang LI, Martin DR, Fung-Leung WP, Teh HS, Miller RG. Zhang LI, et al. J Immunol. 1992 Jun 15;148(12):3740-5. J Immunol. 1992. PMID: 1534823
Cited by
- Hepatocyte entry leads to degradation of autoreactive CD8 T cells.
Benseler V, Warren A, Vo M, Holz LE, Tay SS, Le Couteur DG, Breen E, Allison AC, van Rooijen N, McGuffog C, Schlitt HJ, Bowen DG, McCaughan GW, Bertolino P. Benseler V, et al. Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16735-40. doi: 10.1073/pnas.1112251108. Epub 2011 Sep 20. Proc Natl Acad Sci U S A. 2011. PMID: 21933957 Free PMC article. - Self-tolerance and the composition of T cell repertoire.
Sherman LA, Morgan DJ, Nugent CT, Hernandez FJ, Kreuwel HT, Murtaza A, Ko A, Biggs J. Sherman LA, et al. Immunol Res. 2000;21(2-3):305-13. doi: 10.1385/IR:21:2-3:305. Immunol Res. 2000. PMID: 10852131 Review. - Ontogeny of T cell tolerance to peripherally expressed antigens.
Morgan DJ, Kurts C, Kreuwel HT, Holst KL, Heath WR, Sherman LA. Morgan DJ, et al. Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3854-8. doi: 10.1073/pnas.96.7.3854. Proc Natl Acad Sci U S A. 1999. PMID: 10097127 Free PMC article. - Viral and bacterial infections interfere with peripheral tolerance induction and activate CD8+ T cells to cause immunopathology.
Ehl S, Hombach J, Aichele P, Rülicke T, Odermatt B, Hengartner H, Zinkernagel R, Pircher H. Ehl S, et al. J Exp Med. 1998 Mar 2;187(5):763-74. doi: 10.1084/jem.187.5.763. J Exp Med. 1998. PMID: 9480986 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials