Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: novel targets for cAMP/cGMP function - PubMed (original) (raw)

Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: novel targets for cAMP/cGMP function

P A Kingston et al. Proc Natl Acad Sci U S A. 1996.

Abstract

Cyclic nucleotide-gated (CNG) channels are Ca(2+)-permeable, nonspecific cation channels that can be activated through direct interaction with cAMP and/or cGMP. Recent electrophysiological evidence for these channels in cultured hippocampal neurons prompted us to investigate the expression of CNG channel genes in hippocampus. PCR amplification detected the expression of transcripts for subunit 1 of both the rod photoreceptor (RCNGC1) and the olfactory receptor cell (OCNGC1) subtype of CNG channel in adult rat hippocampus. In situ hybridization detected expression of both channel subtypes in most principal neurons, including pyramidal cells of the CA1 through CA3 regions and granule cells of the dentate gyrus. From the hybridization patterns, we conclude that the two genes are colocalized in individual neurons. Comparison of the patterns of expression of type 1 cGMP-dependent protein kinase and the CNG channels suggests that hippocampal neurons can respond to changes in cGMP levels with both rapid changes in CNG channel activity and slower changes induced by phosphorylation. Future models of hippocampal function should include CNG channels and their effects on both electrical responses and intracellular Ca2+ levels.

PubMed Disclaimer

Cited by

Mutating three residues in the bovine rod cyclic nucleotide-activated channel can switch a nucleotide from inactive to active.
Scott SP, Cummings J, Joe JC, Tanaka JC. Scott SP, et al. Biophys J. 2000 May;78(5):2321-33. doi: 10.1016/S0006-3495(00)76778-6. Biophys J. 2000. PMID: 10777730 Free PMC article.
Alteration in Cngb1 Expression upon Maternal Immune Activation in a Mouse Model and Its Possible Association with Schizophrenia Susceptibility.
Lee H, Kang SW, Jeong H, Kwon JT, Kim YO, Kim HJ. Lee H, et al. Clin Psychopharmacol Neurosci. 2021 Nov 30;19(4):618-627. doi: 10.9758/cpn.2021.19.4.618. Clin Psychopharmacol Neurosci. 2021. PMID: 34690117 Free PMC article.
Movement of gating machinery during the activation of rod cyclic nucleotide-gated channels.
Brown RL, Snow SD, Haley TL. Brown RL, et al. Biophys J. 1998 Aug;75(2):825-33. doi: 10.1016/s0006-3495(98)77571-x. Biophys J. 1998. PMID: 9675183 Free PMC article.
Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channels.
Zong X, Zucker H, Hofmann F, Biel M. Zong X, et al. EMBO J. 1998 Jan 15;17(2):353-62. doi: 10.1093/emboj/17.2.353. EMBO J. 1998. PMID: 9430627 Free PMC article.
Mutation of a single residue in the S2-S3 loop of CNG channels alters the gating properties and sensitivity to inhibitors.
Crary JI, Dean DM, Maroof F, Zimmerman AL. Crary JI, et al. J Gen Physiol. 2000 Dec;116(6):769-80. doi: 10.1085/jgp.116.6.769. J Gen Physiol. 2000. PMID: 11099346 Free PMC article.

References

1. Rev Physiol Biochem Pharmacol. 1975;74:1-103 - PubMed
1. Neuron. 1989 Jul;3(1):71-9 - PubMed
1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
1. Proc Natl Acad Sci U S A. 1981 Jan;78(1):653-7 - PubMed
1. Nature. 1990 Jul 19;346(6281):269-71 - PubMed

Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: novel targets for cAMP/cGMP function - PubMed (original) (raw)