Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae - PubMed (original) (raw)

Effects of mutations of RAD50, RAD51, RAD52, and related genes on illegitimate recombination in Saccharomyces cerevisiae

Y Tsukamoto et al. Genetics. 1996 Feb.

Abstract

To examine the mechanism of illegitimate recombination in Saccharomyces cerevisiae, we have developed a plasmid system for quantitative analysis of deletion formation. A can1 cyh2 cell carrying two negative selection markers, the CAN1 and CYH2 genes, on a YCp plasmid is sensitive to canavanine and cycloheximide, but the cell becomes resistant to both drugs when the plasmid has a deletion over the CAN1 and CYH2 genes. Structural analysis of the recombinant plasmids obtained from the resistant cells showed that the plasmids had deletions at various sites of the CAN1-CYH2 region and there were only short regions of homology (1-5 bp) at the recombination junctions. The results indicated that the deletion detected in this system were formed by illegitimate recombination. Study on the effect of several rad mutations showed that the recombination rate was reduced by 30-, 10-, 10-, and 10-fold in the rad52, rad50, mre11, and xrs2 mutants, respectively, while in the rad51, 54, 55, and 57 mutants, the rate was comparable to that in the wild-type strain. The rad52 mutation did not affect length of homology at junction sites of illegitimate recombination.

PubMed Disclaimer

Similar articles

• Budding yeast Rad50, Mre11, Xrs2, and Hdf1, but not Rad52, are involved in the formation of deletions on a dicentric plasmid.
  Tsukamoto Y, Kato J, Ikeda H. Tsukamoto Y, et al. Mol Gen Genet. 1997 Aug;255(5):543-7. doi: 10.1007/s004380050527. Mol Gen Genet. 1997. PMID: 9294039
• RAD50 and RAD51 define two pathways that collaborate to maintain telomeres in the absence of telomerase.
  Le S, Moore JK, Haber JE, Greider CW. Le S, et al. Genetics. 1999 May;152(1):143-52. doi: 10.1093/genetics/152.1.143. Genetics. 1999. PMID: 10224249 Free PMC article.
• Repair of endonuclease-induced double-strand breaks in Saccharomyces cerevisiae: essential role for genes associated with nonhomologous end-joining.
  Lewis LK, Westmoreland JW, Resnick MA. Lewis LK, et al. Genetics. 1999 Aug;152(4):1513-29. doi: 10.1093/genetics/152.4.1513. Genetics. 1999. PMID: 10430580 Free PMC article.
• Recombination proteins in yeast.
  Krogh BO, Symington LS. Krogh BO, et al. Annu Rev Genet. 2004;38:233-71. doi: 10.1146/annurev.genet.38.072902.091500. Annu Rev Genet. 2004. PMID: 15568977 Review.
• Functions of the yeast meiotic recombination genes, MRE11 and MRE2.
  Ogawa H, Johzuka K, Nakagawa T, Leem SH, Hagihara AH. Ogawa H, et al. Adv Biophys. 1995;31:67-76. doi: 10.1016/0065-227x(95)99383-z. Adv Biophys. 1995. PMID: 7625279 Review.

Cited by

• Cell biology of mitotic recombination.
  Lisby M, Rothstein R. Lisby M, et al. Cold Spring Harb Perspect Biol. 2015 Mar 2;7(3):a016535. doi: 10.1101/cshperspect.a016535. Cold Spring Harb Perspect Biol. 2015. PMID: 25731763 Free PMC article. Review.
• A microhomology-mediated break-induced replication model for the origin of human copy number variation.
  Hastings PJ, Ira G, Lupski JR. Hastings PJ, et al. PLoS Genet. 2009 Jan;5(1):e1000327. doi: 10.1371/journal.pgen.1000327. Epub 2009 Jan 30. PLoS Genet. 2009. PMID: 19180184 Free PMC article. Review.
• Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae.
  Yu J, Marshall K, Yamaguchi M, Haber JE, Weil CF. Yu J, et al. Mol Cell Biol. 2004 Feb;24(3):1351-64. doi: 10.1128/MCB.24.3.1351-1364.2004. Mol Cell Biol. 2004. PMID: 14729978 Free PMC article.
• Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres.
  Tomita K, Matsuura A, Caspari T, Carr AM, Akamatsu Y, Iwasaki H, Mizuno K, Ohta K, Uritani M, Ushimaru T, Yoshinaga K, Ueno M. Tomita K, et al. Mol Cell Biol. 2003 Aug;23(15):5186-97. doi: 10.1128/MCB.23.15.5186-5197.2003. Mol Cell Biol. 2003. PMID: 12861005 Free PMC article.
• mre11S--a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis.
  Nairz K, Klein F. Nairz K, et al. Genes Dev. 1997 Sep 1;11(17):2272-90. doi: 10.1101/gad.11.17.2272. Genes Dev. 1997. PMID: 9303542 Free PMC article.

References

1. 1. Nature. 1995 Jan 5;373(6509):84-6 - PubMed
2. 1. Genetics. 1988 Oct;120(2):367-77 - PubMed
3. 1. Mol Cell Biol. 1994 Jul;14(7):4493-500 - PubMed
4. 1. J Bacteriol. 1971 Mar;105(3):864-72 - PubMed
5. 1. Genetics. 1992 Nov;132(3):651-64 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Saccharomyces Genome Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal