Characterization of manganese peroxidases from the hyperlignolytic fungus IZU-154 - PubMed (original) (raw)
Comparative Study
Characterization of manganese peroxidases from the hyperlignolytic fungus IZU-154
M Matsubara et al. Appl Environ Microbiol. 1996 Nov.
Abstract
Four isozymes of manganese peroxidase (MnP) were identified in the culture fluid of the hyperlignolytic fungus IZU-154 under nitrogen starvation conditions. One of them was purified and characterized kinetically. The specific activity and Kcat/K(m) value of the MnP from IZU-154 were 1.6 times higher than those of the MnP from a typical lignin-degrading fungus, Phanerochaete chrysosporium. Two cDNAs encoding MnP isozymes from IZU-154 were isolated. The coding sequence of the two cDNAs, IZ-MnP1 cDNA and IZ-MnP2 cDNA, were 1,152 (384 amino acids) and 1,155 (385 amino acids) bp in length, respectively. They exhibit 96.2% identity at the nucleotide level and 95.1% identity at the amino acid level. Southern blot analysis indicated that two MnP isozyme genes exist in IZU-154 genomic DNA. The primary structures of two MnPs from IZU-154 were similar to those of MnPs from P. chrysosporium. The amino acid sequences including the important residues identified in MnPs from P. chrysosporium, such as the manganese-binding residues, the calcium-binding residues, the disulfide bonds, and the N-glycosylation site, were conserved in the two deduced IZ-MnPs. However, several discrepancies were found in the context around the distal histidine residue between MnP from IZU-154 and MnP from P. chrysosporium, which likely led to the difference in the kinetic parameters for MnP function.
Similar articles
- Characterization of a cDNA encoding a manganese peroxidase, from the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Pribnow D, Mayfield MB, Nipper VJ, Brown JA, Gold MH. Pribnow D, et al. J Biol Chem. 1989 Mar 25;264(9):5036-40. J Biol Chem. 1989. PMID: 2925681 - Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium.
Reddy CA, D'Souza TM. Reddy CA, et al. FEMS Microbiol Rev. 1994 Mar;13(2-3):137-52. doi: 10.1111/j.1574-6976.1994.tb00040.x. FEMS Microbiol Rev. 1994. PMID: 8167033 Review. - Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Gold MH, Alic M. Gold MH, et al. Microbiol Rev. 1993 Sep;57(3):605-22. doi: 10.1128/mr.57.3.605-622.1993. Microbiol Rev. 1993. PMID: 8246842 Free PMC article. Review.
Cited by
- Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?
Wei R, Zimmermann W. Wei R, et al. Microb Biotechnol. 2017 Nov;10(6):1308-1322. doi: 10.1111/1751-7915.12710. Epub 2017 Mar 28. Microb Biotechnol. 2017. PMID: 28371373 Free PMC article. Review. - Purification and characterization of a novel peroxidase from Geotrichum candidum dec 1 involved in decolorization of dyes.
Kim SJ, Shoda M. Kim SJ, et al. Appl Environ Microbiol. 1999 Mar;65(3):1029-35. doi: 10.1128/AEM.65.3.1029-1035.1999. Appl Environ Microbiol. 1999. PMID: 10049859 Free PMC article. - Lignolytic Enzymes of a Mushroom Stereum ostrea Isolated from Wood Logs.
Praveen K, Viswanath B, Usha KY, Pallavi H, Venkata Subba Reddy G, Naveen M, Rajasekhar Reddy B. Praveen K, et al. Enzyme Res. 2011;2011:749518. doi: 10.4061/2011/749518. Epub 2011 Sep 20. Enzyme Res. 2011. PMID: 21941632 Free PMC article. - Plastic-Degrading Potential across the Global Microbiome Correlates with Recent Pollution Trends.
Zrimec J, Kokina M, Jonasson S, Zorrilla F, Zelezniak A. Zrimec J, et al. mBio. 2021 Oct 26;12(5):e0215521. doi: 10.1128/mBio.02155-21. Epub 2021 Oct 26. mBio. 2021. PMID: 34700384 Free PMC article. - Purification and characterization of a nylon-degrading enzyme.
Deguchi T, Kitaoka Y, Kakezawa M, Nishida T. Deguchi T, et al. Appl Environ Microbiol. 1998 Apr;64(4):1366-71. doi: 10.1128/AEM.64.4.1366-1371.1998. Appl Environ Microbiol. 1998. PMID: 9546174 Free PMC article.
References
- Biochem Biophys Res Commun. 1983 Aug 12;114(3):1077-83 - PubMed
- Biochem Biophys Res Commun. 1991 Apr 15;176(1):269-75 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- Appl Environ Microbiol. 1993 Dec;59(12):4017-23 - PubMed
- Arch Biochem Biophys. 1986 Feb 1;244(2):750-65 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources