Alternative splicing of human inducible nitric-oxide synthase mRNA. tissue-specific regulation and induction by cytokines - PubMed (original) (raw)
. 1996 Oct 25;271(43):27184-7.
doi: 10.1074/jbc.271.43.27184.
Affiliations
- PMID: 8900212
- DOI: 10.1074/jbc.271.43.27184
Free article
Alternative splicing of human inducible nitric-oxide synthase mRNA. tissue-specific regulation and induction by cytokines
N T Eissa et al. J Biol Chem. 1996.
Free article
Abstract
Human inducible nitric-oxide synthase (iNOS) is responsible for nitric oxide synthesis in response to inflammatory mediators. The human iNOS gene, containing 26 exons, encodes a protein of 131 kDa. This study was aimed at investigating the presence of alternative splicing of human iNOS mRNA. Total RNA from human alveolar macrophages, nasal and bronchial epithelial cells, and several human tissues was transcribed to cDNA and analyzed using polymerase chain reaction with specific primers for segmental analysis of the iNOS gene. Four sites of alternative splicing were identified by sequence analysis; these included deletion of: (i) exon 5; (ii) exons 8 and 9; (iii) exons 9, 10, and 11; and (iv) exons 15 and 16. The deduced amino acid sequences of the novel iNOS cDNAs predict one truncated protein (resulting from exon 5 deletion) and three iNOS proteins with in-frame deletions. Southern analyses of polymerase chain reaction products were consistent with tissue-specific regulation of alternative splicing. In cultured cells, iNOS induction by cytokines and lipopolysaccharide was associated with an increase in alternatively spliced mRNA transcripts. Because iNOS is active as a dimer, the novel forms of alternatively spliced iNOS may be involved in regulation of nitric oxide synthesis.
Similar articles
- Post-transcriptional regulation of inducible nitric oxide synthase in chronic lymphocytic leukemia B cells in pro- and antiapoptotic culture conditions.
Tiscornia AC, Cayota A, Landoni AI, Brito C, Oppezzo P, Vuillier F, Robello C, Dighiero G, Gabús R, Pritsch O. Tiscornia AC, et al. Leukemia. 2004 Jan;18(1):48-56. doi: 10.1038/sj.leu.2403169. Leukemia. 2004. PMID: 14574328 - Structural diversity in the 5'-untranslated region of cytokine-stimulated human inducible nitric oxide synthase mRNA.
Chu SC, Wu HP, Banks TC, Eissa NT, Moss J. Chu SC, et al. J Biol Chem. 1995 May 5;270(18):10625-30. doi: 10.1074/jbc.270.18.10625. J Biol Chem. 1995. PMID: 7537735 - Translational regulation of human neuronal nitric-oxide synthase by an alternatively spliced 5'-untranslated region leader exon.
Newton DC, Bevan SC, Choi S, Robb GB, Millar A, Wang Y, Marsden PA. Newton DC, et al. J Biol Chem. 2003 Jan 3;278(1):636-44. doi: 10.1074/jbc.M209988200. Epub 2002 Oct 25. J Biol Chem. 2003. PMID: 12403769 - The harmony of the spheres: inducible nitric oxide synthase and related genes in pancreatic beta cells.
Eizirik DL, Flodström M, Karlsen AE, Welsh N. Eizirik DL, et al. Diabetologia. 1996 Aug;39(8):875-90. doi: 10.1007/BF00403906. Diabetologia. 1996. PMID: 8858209 Review. - Regulation of the expression of inducible nitric oxide synthase.
Kleinert H, Schwarz PM, Förstermann U. Kleinert H, et al. Biol Chem. 2003 Oct-Nov;384(10-11):1343-64. doi: 10.1515/BC.2003.152. Biol Chem. 2003. PMID: 14669979 Review.
Cited by
- Uncovering Novel Protein Partners of Inducible Nitric Oxide Synthase in Human Testis.
Prabhakara KS, Ganapathy K, Islam KN, Thyagarajan HM, Tiwari KK, Parimi RL, Rashid MB. Prabhakara KS, et al. Biomolecules. 2024 Mar 24;14(4):388. doi: 10.3390/biom14040388. Biomolecules. 2024. PMID: 38672406 Free PMC article. - A specific, non-immune system-related isoform of the human inducible nitric oxide synthase is expressed during differentiation of human stem cells into various cell types.
Gather F, Ihrig-Biedert I, Kohlhas P, Krutenko T, Peitz M, Brüstle O, Pautz A, Kleinert H. Gather F, et al. Cell Commun Signal. 2022 Apr 7;20(1):47. doi: 10.1186/s12964-022-00855-x. Cell Commun Signal. 2022. PMID: 35392923 Free PMC article. - Nitric oxide signalling in kidney regulation and cardiometabolic health.
Carlström M. Carlström M. Nat Rev Nephrol. 2021 Sep;17(9):575-590. doi: 10.1038/s41581-021-00429-z. Epub 2021 Jun 1. Nat Rev Nephrol. 2021. PMID: 34075241 Free PMC article. Review. - S-nitrosothiols and H2S donors: Potential chemo-therapeutic agents in cancer.
Reis AKCA, Stern A, Monteiro HP. Reis AKCA, et al. Redox Biol. 2019 Oct;27:101190. doi: 10.1016/j.redox.2019.101190. Epub 2019 Apr 5. Redox Biol. 2019. PMID: 30981679 Free PMC article. Review. - Molecular Mechanisms of Nitric Oxide in Cancer Progression, Signal Transduction, and Metabolism.
Somasundaram V, Basudhar D, Bharadwaj G, No JH, Ridnour LA, Cheng RYS, Fujita M, Thomas DD, Anderson SK, McVicar DW, Wink DA. Somasundaram V, et al. Antioxid Redox Signal. 2019 Mar 10;30(8):1124-1143. doi: 10.1089/ars.2018.7527. Epub 2018 May 2. Antioxid Redox Signal. 2019. PMID: 29634348 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources