Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole - PubMed (original) (raw)

Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole

S V Scott et al. Proc Natl Acad Sci U S A. 1996.

Abstract

The vacuolar protein aminopeptidase I (API) uses a novel cytoplasm-to-vacuole targeting (Cvt) pathway. Complementation analysis of yeast mutants defective for cytoplasm-to-vacuole protein targeting (cvt) and autophagy (apg) revealed seven overlapping complementation groups between these two sets of mutants. In addition, all 14 apg complementation groups are defective in the delivery of API to the vacuole. Similarly, the majority of nonoverlapping cvt complementation groups appear to be at least partially defective in autophagy. Kinetic analyses of protein delivery rates indicate that autophagic protein uptake is induced by nitrogen starvation, whereas Cvt is a constitutive biosynthetic pathway. However, the machinery governing Cvt is affected by nitrogen starvation as targeting defects resulting from API overexpression can be rescued by induction of autophagy.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1988 Nov;8(11):4936-48 - PubMed
    1. Cell Struct Funct. 1995 Dec;20(6):465-71 - PubMed
    1. EMBO J. 1989 Aug;8(8):2241-50 - PubMed
    1. J Biol Chem. 1990 Oct 25;265(30):18447-53 - PubMed
    1. Microbiol Rev. 1990 Sep;54(3):266-92 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources