Molecular analysis of microflora associated with dentoalveolar abscesses - PubMed (original) (raw)
Molecular analysis of microflora associated with dentoalveolar abscesses
D Dymock et al. J Clin Microbiol. 1996 Mar.
Abstract
The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cultured bacteria was used to detect putative unculturable bacteria. Clones representative of five predominant groups of uncultured organisms were sequenced. Two were identified as Porphyromonas gingivalis and Prevotella oris, and one was found to be closely related to Peptostreptococcus micros. The remaining two clones did not correspond to known, previously sequenced organisms. One was related to Zoogloea ramigera, a species of aerobic waterborne organisms, while the other was distantly related to the genus Prevotella. This study has demonstrated the possibility of the characterization of microflora associated with human infection by molecular methods without the inherent biases of culture.
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References
- Oral Surg Oral Med Oral Pathol. 1967 Mar;23(3):277-93 - PubMed
- Arch Oral Biol. 1963 May-Jun;8:275-80 - PubMed
- Ann Otol Rhinol Laryngol. 1982 Jan-Feb;91(1 Pt 1):41-3 - PubMed
- J Clin Microbiol. 1982 May;15(5):964-66 - PubMed
- J Med Microbiol. 1986 Mar;21(2):101-4 - PubMed
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