HIV type 1 RNA expression in bone marrows of patients with a spectrum of disease - PubMed (original) (raw)
HIV type 1 RNA expression in bone marrows of patients with a spectrum of disease
B Weiser et al. AIDS Res Hum Retroviruses. 1996.
Abstract
HIV-1-infected individuals at various stages of disease harbor virus in their lymphoid organs, which serve as reservoirs of viral replication throughout the course of infection. Hematologic abnormalities are extremely common in HIV-1-infected individuals and occur at all stages of disease. To determine if the bone marrow is a reservoir of HIV-1 in vivo and if active HIV-1 RNA expression in that site is related to hematologic disease in infected individuals, we examined HIV-1 RNA expression in bone marrow biopsies from 37 patients with a broad spectrum of hematologic and HIV-1-related disease. To detect HIV-1 RNA expression, we performed in situ hybridization. Double-label in situ hybridization-immunohistochemistry was used for precise identification of the type of cell expressing viral RNA. Six of 37 (16%) patients demonstrated HIV-1 RNA expression in the bone marrow. Double-label analysis performed on two marrows localized HIV-1 RNA to cells of the macrophage lineage. Active HIV-1 expression correlated with advanced HIV-1-related disease and CD4 cell depletion rather than a specific hematologic or clinical diagnosis. These data suggest that although the bone marrow does not serve as a reservoir of viral expression throughout the course of infection as do the lymphoid organs, HIV-1-expressing cells are present in the bone marrow during late stages of disease. These data also suggest that hematologic abnormalities in the majority of infected individuals may result from indirect effects of HIV-1 such as cytokine dysregulation rather than HIV-1 expression in the bone marrow itself.
Similar articles
- Isolated bone marrow manifestation of HIV-associated Hodgkin lymphoma.
Ponzoni M, Fumagalli L, Rossi G, Freschi M, Re A, Viganò MG, Guidoboni M, Dolcetti R, McKenna RW, Facchetti F. Ponzoni M, et al. Mod Pathol. 2002 Dec;15(12):1273-8. doi: 10.1097/01.MP.0000037311.56159.13. Mod Pathol. 2002. PMID: 12481007 - Nonlymphoid reservoirs of HIV replication in children with chronic-progressive disease.
Brodie SJ. Brodie SJ. J Leukoc Biol. 2000 Sep;68(3):351-9. J Leukoc Biol. 2000. PMID: 10985251 Retracted. - Follicular dendritic cells are a major reservoir for human immunodeficiency virus type 1 in lymphoid tissues facilitating infection of CD4+ T-helper cells.
Spiegel H, Herbst H, Niedobitek G, Foss HD, Stein H. Spiegel H, et al. Am J Pathol. 1992 Jan;140(1):15-22. Am J Pathol. 1992. PMID: 1530997 Free PMC article. - HIV-1 infection and hematologic picture.
Re MC, Zauli G, Furlini G, Giovannini M, La Placa M. Re MC, et al. Microbiologica. 1991 Apr;14(2):165-76. Microbiologica. 1991. PMID: 1857267 Review. - [Hematological changes associated with human immunodeficiency virus (HIV-1) infection].
Møller T, Hasselbalch HC. Møller T, et al. Ugeskr Laeger. 1993 May 10;155(19):1442-6. Ugeskr Laeger. 1993. PMID: 8316970 Review. Danish.
Cited by
- HIV DNA in circulating monocytes as a mechanism to dementia and other HIV complications.
Valcour VG, Shiramizu BT, Shikuma CM. Valcour VG, et al. J Leukoc Biol. 2010 Apr;87(4):621-6. doi: 10.1189/jlb.0809571. Epub 2010 Feb 3. J Leukoc Biol. 2010. PMID: 20130221 Free PMC article. Review. - Detection of JC virus DNA and proteins in the bone marrow of HIV-positive and HIV-negative patients: implications for viral latency and neurotropic transformation.
Tan CS, Dezube BJ, Bhargava P, Autissier P, Wüthrich C, Miller J, Koralnik IJ. Tan CS, et al. J Infect Dis. 2009 Mar 15;199(6):881-8. doi: 10.1086/597117. J Infect Dis. 2009. PMID: 19434914 Free PMC article. - Bone marrow diffusion measures correlate with dementia severity in HIV patients.
Ragin AB, Wu Y, Storey P, Cohen BA, Edelman RR, Epstein LG, Gartner S. Ragin AB, et al. AJNR Am J Neuroradiol. 2006 Mar;27(3):589-92. AJNR Am J Neuroradiol. 2006. PMID: 16551998 Free PMC article. - V3 loop-determined coreceptor preference dictates the dynamics of CD4+-T-cell loss in simian-human immunodeficiency virus-infected macaques.
Ho SH, Shek L, Gettie A, Blanchard J, Cheng-Mayer C. Ho SH, et al. J Virol. 2005 Oct;79(19):12296-303. doi: 10.1128/JVI.79.19.12296-12303.2005. J Virol. 2005. PMID: 16160156 Free PMC article. - Compartmentalization of human immunodeficiency virus type 1 between blood monocytes and CD4+ T cells during infection.
Fulcher JA, Hwangbo Y, Zioni R, Nickle D, Lin X, Heath L, Mullins JI, Corey L, Zhu T. Fulcher JA, et al. J Virol. 2004 Aug;78(15):7883-93. doi: 10.1128/JVI.78.15.7883-7893.2004. J Virol. 2004. PMID: 15254161 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials