PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples - PubMed (original) (raw)

PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples

R F Wang et al. Appl Environ Microbiol. 1996 Apr.

Abstract

PCR procedures based on 16S rRNA gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. Fusobacterium prausnitzii, Peptostreptococcus productus, and Clostridium clostridiiforme had high PCR titers (the maximum dilutions for positive PCR results ranged from 10(-3) to 10(-8)) in all of the human and animal fecal samples tested. Bacteroides thetaiotaomicron, Bacteroides vulgatus, and Eubacterium limosum also showed higher PCR titers (10(-2) to 10(-6)) in adult human feces. The other bacteria tested, including Escherichia coli, Bifidobacterium adolescentis, Bifidobacterium longum, Lactobacillus acidophilus, Eubacterium biforme, and Bacteroides distasonis, were either at low PCR titers (less than 10(-2)) or not detected by PCR. The reported PCR procedure including the fecal sample preparation method is simplified and rapid and eliminates the DNA isolation steps.

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References

    1. Gut. 1969 May;10(5):334-5 - PubMed
    1. Appl Environ Microbiol. 1995 Sep;61(9):3202-7 - PubMed
    1. Appl Microbiol. 1974 May;27(5):961-79 - PubMed
    1. Carcinogenesis. 1982;3(11):1255-60 - PubMed
    1. Drug Metab Dispos. 1984 May-Jun;12(3):330-6 - PubMed

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