Isolation of the most immature population of murine fetal thymocytes that includes progenitors capable of generating T, B, and myeloid cells - PubMed (original) (raw)

Comparative Study

Isolation of the most immature population of murine fetal thymocytes that includes progenitors capable of generating T, B, and myeloid cells

N Hattori et al. J Exp Med. 1996.

Abstract

Thymus cells of murine fetuses at day 12 of gestation are exclusively of the CD3-CD4-CD8-CD44+CD25- phenotype, which is known as a hallmark of the most immature subset of thymus cells. In the present study, we show that day 12 fetal thymus (FT) cells express Fc gamma RII/ III (FcR) at a broad range of levels on their surface. The FcR+ FT cells seem to represent T lineage cells, because a large majority of them express the T lineage specific transcription factors TCF-1 and GATA-3 as well as CD3 epsilon in the cytoplasm. Also shown is that the FcR- population contains progenitors capable of developing into not only T cells but also B and myeloid cells, whereas FcR+ progenitors are mostly committed to the T lineage. These findings indicate that thymic T lineage cells express FcR on their surface at the earliest stage of differentiation, and thus FcR is a useful marker in isolating the most immature population of murine FT cells.

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Isolation of the most immature population of murine fetal thymocytes that includes progenitors capable of generating T, B, and myeloid cells - PubMed (original) (raw)