Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation - PubMed (original) (raw)

Comparative Study

Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation

J L Puglisi et al. Am J Physiol. 1996 May.

Abstract

The relative contributions of the different Ca transport systems involved in cardiac relaxation were evaluated at 25 and 35 degrees C in isolated rabbit, ferret, and cat ventricular myocytes during twitches, caffeine-induced contractures in normal Tyrode solution, and caffeine-induced contractures in Na- and Ca-free solution. The time course of intracellular [Ca] decline these contractions in rabbit ventricular myocytes allowed estimates of the relative contributions of the sarcoplasmic reticulum (SR) Ca pump, Na/Ca exchange, sarcolemmal Ca pump, and the mitochondrial calcium uniporter (with the latter two considered together as "slow mechanisms"). The percent contributions of the SR Ca pump, the Na/Ca exchange, and the slow mechanisms were 70, 27 and 3% at 25 degrees C and 74, 23, and 3% at 35 degrees C. Warming from 25 to 35 degrees C decreases twitch contractions in rabbit and ferret myocytes and caffeine-induced contractures in normal Tyrode solution and Na- and Ca-free solution in all species. In contrast, in cat myocytes warming increased twitches, possibly because of a stronger effect of temperature on Ca influx. We conclude that increased temperature accelerates all of the Ca transport systems involved in relaxation. Despite large changes in each Ca transport system with warming, the relative contributions during relaxation remain similar at physiological temperature.

PubMed Disclaimer

Similar articles

• Relaxation in ferret ventricular myocytes: unusual interplay among calcium transport systems.
  Bassani RA, Bassani JW, Bers DM. Bassani RA, et al. J Physiol. 1994 Apr 15;476(2):295-308. doi: 10.1113/jphysiol.1994.sp020131. J Physiol. 1994. PMID: 8046644 Free PMC article.
• Relaxation in rabbit and rat cardiac cells: species-dependent differences in cellular mechanisms.
  Bassani JW, Bassani RA, Bers DM. Bassani JW, et al. J Physiol. 1994 Apr 15;476(2):279-93. doi: 10.1113/jphysiol.1994.sp020130. J Physiol. 1994. PMID: 8046643 Free PMC article.
• Mitochondrial and sarcolemmal Ca2+ transport reduce [Ca2+]i during caffeine contractures in rabbit cardiac myocytes.
  Bassani RA, Bassani JW, Bers DM. Bassani RA, et al. J Physiol. 1992;453:591-608. doi: 10.1113/jphysiol.1992.sp019246. J Physiol. 1992. PMID: 1464847 Free PMC article.
• Na-Ca exchange and Ca fluxes during contraction and relaxation in mammalian ventricular muscle.
  Bers DM, Bassani JW, Bassani RA. Bers DM, et al. Ann N Y Acad Sci. 1996 Apr 15;779:430-42. doi: 10.1111/j.1749-6632.1996.tb44818.x. Ann N Y Acad Sci. 1996. PMID: 8659859 Review.
• Measurement of calcium transport in heart using modern approaches.
  Bers DM. Bers DM. New Horiz. 1996 Feb;4(1):36-44. New Horiz. 1996. PMID: 8689274 Review.

Cited by

• S-Nitrosylation of Calcium-Handling Proteins in Cardiac Adrenergic Signaling and Hypertrophy.
  Irie T, Sips PY, Kai S, Kida K, Ikeda K, Hirai S, Moazzami K, Jiramongkolchai P, Bloch DB, Doulias PT, Armoundas AA, Kaneki M, Ischiropoulos H, Kranias E, Bloch KD, Stamler JS, Ichinose F. Irie T, et al. Circ Res. 2015 Oct 9;117(9):793-803. doi: 10.1161/CIRCRESAHA.115.307157. Epub 2015 Aug 10. Circ Res. 2015. PMID: 26259881 Free PMC article.
• Spontaneous Ca waves in ventricular myocytes from failing hearts depend on Ca(2+)-calmodulin-dependent protein kinase II.
  Curran J, Brown KH, Santiago DJ, Pogwizd S, Bers DM, Shannon TR. Curran J, et al. J Mol Cell Cardiol. 2010 Jul;49(1):25-32. doi: 10.1016/j.yjmcc.2010.03.013. Epub 2010 Mar 29. J Mol Cell Cardiol. 2010. PMID: 20353795 Free PMC article.
• Distinguishing between overdrive excited and suppressed ventricular beats in guinea pig ventricular myocardium.
  Greer-Short A, Poelzing S. Greer-Short A, et al. Front Physiol. 2015 Feb 18;6:14. doi: 10.3389/fphys.2015.00014. eCollection 2015. Front Physiol. 2015. PMID: 25741282 Free PMC article.
• Skeletal and cardiac muscle calcium transport regulation in health and disease.
  Valentim MA, Brahmbhatt AN, Tupling AR. Valentim MA, et al. Biosci Rep. 2022 Dec 22;42(12):BSR20211997. doi: 10.1042/BSR20211997. Biosci Rep. 2022. PMID: 36413081 Free PMC article. Review.
• Diminished function and expression of the cardiac Na+-Ca2+ exchanger in diabetic rats: implication in Ca2+ overload.
  Hattori Y, Matsuda N, Kimura J, Ishitani T, Tamada A, Gando S, Kemmotsu O, Kanno M. Hattori Y, et al. J Physiol. 2000 Aug 15;527 Pt 1(Pt 1):85-94. doi: 10.1111/j.1469-7793.2000.00085.x. J Physiol. 2000. PMID: 10944172 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal