Regional and ontogenic expression of the NMDA receptor subunit NR2D protein in rat brain using a subunit-specific antibody - PubMed (original) (raw)
Regional and ontogenic expression of the NMDA receptor subunit NR2D protein in rat brain using a subunit-specific antibody
A W Dunah et al. J Neurochem. 1996 Dec.
Abstract
A polyclonal antibody for the NMDA receptor subunit NR2D has been developed that identifies an approximately 160-kDa band on immunoblots from NR2D transfected cells and CNS tissues. No cross-reactivity is seen with other NMDA receptor subunits. The NR2D receptor subunit is N-glycosylated in both brain and transfected cells. Transfected cells expressing NR2D are immunofluorescently labeled, whereas untransfected cells or cells transfected with other NMDA receptor subunit cDNAs are not. Similarly, the NR2D subunit is selectively and quantitatively immunoprecipitated, whereas the NR1, NR2A, or NR2B subunit is not. The relative densities of the NR2D subunit in nine areas of postnatal day 7 and adult rat brains have been determined by quantitative immunoblotting. NR2D was expressed at highest levels in the thalamus, midbrain, medulla, and spinal cord, whereas intermediate levels of this subunit were found in the cortex and hippocampus. Low or undetectable levels were seen in the olfactory bulb, striatum, and cerebellum. Following a peak after the first week of birth, NR2D protein levels decreased by about twofold in adulthood in all rat brain regions examined. More complete ontogenic profiles were determined for the diencephalon, telencephalon, and spinal cord where similar ontogenic patterns were seen. NR2D protein is present at high levels at embryonic stages of development, rises to a peak at postnatal day 7, and decreases but remains measurable during late postnatal life. This study demonstrates the generation and characterization of an antibody selective for the NR2D NMDA receptor subunit as well as a determination of the distribution and ontogenic profile of this subunit in rat brain. The results suggest that native NMDA receptors containing the NR2D subunit may have functional roles not only in the young brain but also in adult brain.
Similar articles
- Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum.
Wang YH, Bosy TZ, Yasuda RP, Grayson DR, Vicini S, Pizzorusso T, Wolfe BB. Wang YH, et al. J Neurochem. 1995 Jul;65(1):176-83. doi: 10.1046/j.1471-4159.1995.65010176.x. J Neurochem. 1995. PMID: 7790859 - Subunit composition of N-methyl-D-aspartate receptors in the central nervous system that contain the NR2D subunit.
Dunah AW, Luo J, Wang YH, Yasuda RP, Wolfe BB. Dunah AW, et al. Mol Pharmacol. 1998 Mar;53(3):429-37. doi: 10.1124/mol.53.3.429. Mol Pharmacol. 1998. PMID: 9495808 - The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR1.
Petralia RS, Wang YX, Wenthold RJ. Petralia RS, et al. J Neurosci. 1994 Oct;14(10):6102-20. doi: 10.1523/JNEUROSCI.14-10-06102.1994. J Neurosci. 1994. PMID: 7931566 Free PMC article. - The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B).
Luo J, Wang Y, Yasuda RP, Dunah AW, Wolfe BB. Luo J, et al. Mol Pharmacol. 1997 Jan;51(1):79-86. doi: 10.1124/mol.51.1.79. Mol Pharmacol. 1997. PMID: 9016349 - Subunit characterization of NMDA receptors.
Stephenson FA. Stephenson FA. Curr Drug Targets. 2001 Sep;2(3):233-9. doi: 10.2174/1389450013348461. Curr Drug Targets. 2001. PMID: 11554550 Review.
Cited by
- GluN2C/GluN2D subunit-selective NMDA receptor potentiator CIQ reverses MK-801-induced impairment in prepulse inhibition and working memory in Y-maze test in mice.
Suryavanshi PS, Ugale RR, Yilmazer-Hanke D, Stairs DJ, Dravid SM. Suryavanshi PS, et al. Br J Pharmacol. 2014 Feb;171(3):799-809. doi: 10.1111/bph.12518. Br J Pharmacol. 2014. PMID: 24236947 Free PMC article. - Biochemical studies of the structure and function of the N-methyl-D-aspartate subtype of glutamate receptors.
Dunah AW, Yasuda RP, Luo J, Wang Y, Prybylowski KL, Wolfe BB. Dunah AW, et al. Mol Neurobiol. 1999 Apr;19(2):151-79. doi: 10.1007/BF02743658. Mol Neurobiol. 1999. PMID: 10371467 Review. - Alternative RNA splicing of the NMDA receptor NR1 mRNA in the neurons of the teleost electrosensory system.
Bottai D, Maler L, Dunn RJ. Bottai D, et al. J Neurosci. 1998 Jul 15;18(14):5191-202. doi: 10.1523/JNEUROSCI.18-14-05191.1998. J Neurosci. 1998. PMID: 9651202 Free PMC article. - Modulation of NMDA receptors in the cerebellum. 1. Properties of the NMDA receptor that modulate its function.
Llansola M, Sanchez-Perez A, Cauli O, Felipo V. Llansola M, et al. Cerebellum. 2005;4(3):154-61. doi: 10.1080/14734220510007996. Cerebellum. 2005. PMID: 16147947 Review. - Activity-dependent regulation of NMDA receptors in substantia nigra dopaminergic neurones.
Wild AR, Jones S, Gibb AJ. Wild AR, et al. J Physiol. 2014 Feb 15;592(4):653-68. doi: 10.1113/jphysiol.2013.267310. Epub 2013 Dec 16. J Physiol. 2014. PMID: 24344168 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources