Regional and ontogenic expression of the NMDA receptor subunit NR2D protein in rat brain using a subunit-specific antibody - PubMed (original) (raw)
Regional and ontogenic expression of the NMDA receptor subunit NR2D protein in rat brain using a subunit-specific antibody
A W Dunah et al. J Neurochem. 1996 Dec.
Abstract
A polyclonal antibody for the NMDA receptor subunit NR2D has been developed that identifies an approximately 160-kDa band on immunoblots from NR2D transfected cells and CNS tissues. No cross-reactivity is seen with other NMDA receptor subunits. The NR2D receptor subunit is N-glycosylated in both brain and transfected cells. Transfected cells expressing NR2D are immunofluorescently labeled, whereas untransfected cells or cells transfected with other NMDA receptor subunit cDNAs are not. Similarly, the NR2D subunit is selectively and quantitatively immunoprecipitated, whereas the NR1, NR2A, or NR2B subunit is not. The relative densities of the NR2D subunit in nine areas of postnatal day 7 and adult rat brains have been determined by quantitative immunoblotting. NR2D was expressed at highest levels in the thalamus, midbrain, medulla, and spinal cord, whereas intermediate levels of this subunit were found in the cortex and hippocampus. Low or undetectable levels were seen in the olfactory bulb, striatum, and cerebellum. Following a peak after the first week of birth, NR2D protein levels decreased by about twofold in adulthood in all rat brain regions examined. More complete ontogenic profiles were determined for the diencephalon, telencephalon, and spinal cord where similar ontogenic patterns were seen. NR2D protein is present at high levels at embryonic stages of development, rises to a peak at postnatal day 7, and decreases but remains measurable during late postnatal life. This study demonstrates the generation and characterization of an antibody selective for the NR2D NMDA receptor subunit as well as a determination of the distribution and ontogenic profile of this subunit in rat brain. The results suggest that native NMDA receptors containing the NR2D subunit may have functional roles not only in the young brain but also in adult brain.
Similar articles
- Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum.
Wang YH, Bosy TZ, Yasuda RP, Grayson DR, Vicini S, Pizzorusso T, Wolfe BB. Wang YH, et al. J Neurochem. 1995 Jul;65(1):176-83. doi: 10.1046/j.1471-4159.1995.65010176.x. J Neurochem. 1995. PMID: 7790859 - Subunit composition of N-methyl-D-aspartate receptors in the central nervous system that contain the NR2D subunit.
Dunah AW, Luo J, Wang YH, Yasuda RP, Wolfe BB. Dunah AW, et al. Mol Pharmacol. 1998 Mar;53(3):429-37. doi: 10.1124/mol.53.3.429. Mol Pharmacol. 1998. PMID: 9495808 - The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR1.
Petralia RS, Wang YX, Wenthold RJ. Petralia RS, et al. J Neurosci. 1994 Oct;14(10):6102-20. doi: 10.1523/JNEUROSCI.14-10-06102.1994. J Neurosci. 1994. PMID: 7931566 Free PMC article. - The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B).
Luo J, Wang Y, Yasuda RP, Dunah AW, Wolfe BB. Luo J, et al. Mol Pharmacol. 1997 Jan;51(1):79-86. doi: 10.1124/mol.51.1.79. Mol Pharmacol. 1997. PMID: 9016349 - Subunit characterization of NMDA receptors.
Stephenson FA. Stephenson FA. Curr Drug Targets. 2001 Sep;2(3):233-9. doi: 10.2174/1389450013348461. Curr Drug Targets. 2001. PMID: 11554550 Review.
Cited by
- Cocaine enhances NMDA receptor-mediated currents in ventral tegmental area cells via dopamine D5 receptor-dependent redistribution of NMDA receptors.
Schilström B, Yaka R, Argilli E, Suvarna N, Schumann J, Chen BT, Carman M, Singh V, Mailliard WS, Ron D, Bonci A. Schilström B, et al. J Neurosci. 2006 Aug 16;26(33):8549-58. doi: 10.1523/JNEUROSCI.5179-05.2006. J Neurosci. 2006. PMID: 16914681 Free PMC article. - Allosteric modulation of GluN2C/GluN2D-containing NMDA receptors bidirectionally modulates dopamine release: implication for Parkinson's disease.
Zhang X, Feng ZJ, Chergui K. Zhang X, et al. Br J Pharmacol. 2014 Aug;171(16):3938-45. doi: 10.1111/bph.12758. Br J Pharmacol. 2014. PMID: 24818560 Free PMC article. - Glutamate receptor abnormalities in the YAC128 transgenic mouse model of Huntington's disease.
Benn CL, Slow EJ, Farrell LA, Graham R, Deng Y, Hayden MR, Cha JH. Benn CL, et al. Neuroscience. 2007 Jun 29;147(2):354-72. doi: 10.1016/j.neuroscience.2007.03.010. Epub 2007 Jun 4. Neuroscience. 2007. PMID: 17544587 Free PMC article. - Onset of Pup Locomotion Coincides with Loss of NR2C/D-Mediated Cortico-Striatal EPSCs and Dampening of Striatal Network Immature Activity.
Dehorter N, Michel FJ, Marissal T, Rotrou Y, Matrot B, Lopez C, Humphries MD, Hammond C. Dehorter N, et al. Front Cell Neurosci. 2011 Nov 21;5:24. doi: 10.3389/fncel.2011.00024. eCollection 2011. Front Cell Neurosci. 2011. PMID: 22125512 Free PMC article. - Modal gating of GluN1/GluN2D NMDA receptors.
Vance KM, Hansen KB, Traynelis SF. Vance KM, et al. Neuropharmacology. 2013 Aug;71:184-90. doi: 10.1016/j.neuropharm.2013.03.018. Epub 2013 Apr 8. Neuropharmacology. 2013. PMID: 23578394 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources