Effect of telomere length on telomeric gene expression - PubMed (original) (raw)
Effect of telomere length on telomeric gene expression
C N Sprung et al. Nucleic Acids Res. 1996.
Abstract
Telomeres gradually shorten as human somatic cells divide and a correlation has been observed between the average telomere length and cell senescence. It has been proposed that the genes responsible for cell senescence are located near the telomere and are activated when telomere length reaches a critical point. This is consistent with evidence from Saccharomyces cerevisiae, in which genes are regulated differently depending on their distance from the telomere. We investigated the possibility that differential gene expression is conferred by telomere length in human cells. A plasmid containing the neomycin phosphotransferase (neo) gene was transfected into the SV40-transformed human fibroblast cell line LM217. In one transfectant the plasmid was integrated at the telomere of chromosome 13. Subclones of this cell line that had various lengths of telomeric repeat sequences on the end of this chromosome were isolated. No effect on neo gene expression was found when the length of the telomere varied between 25 and 0.5 kb, as demonstrated by colony forming ability, growth rates and RNA blot analysis. These results therefore suggest that putative chromatin structural differences conferred by telomere length do not affect the expression of genes located near telomeres.
Similar articles
- Telomeric transgenes are silenced in adult mouse tissues and embryo fibroblasts but are expressed in embryonic stem cells.
Gao Q, Reynolds GE, Innes L, Pedram M, Jones E, Junabi M, Gao DW, Ricoul M, Sabatier L, Van Brocklin H, Franc BL, Murnane JP. Gao Q, et al. Stem Cells. 2007 Dec;25(12):3085-92. doi: 10.1634/stemcells.2007-0478. Epub 2007 Sep 6. Stem Cells. 2007. PMID: 17823235 - A ribosomal RNA gene promoter at the telomere of a mini-chromosome in Trypanosoma brucei.
Zomerdijk JC, Kieft R, Borst P. Zomerdijk JC, et al. Nucleic Acids Res. 1992 Jun 11;20(11):2725-34. doi: 10.1093/nar/20.11.2725. Nucleic Acids Res. 1992. PMID: 1319572 Free PMC article. - Telomere length and the expression of natural telomeric genes in human fibroblasts.
Ning Y, Xu JF, Li Y, Chavez L, Riethman HC, Lansdorp PM, Weng NP. Ning Y, et al. Hum Mol Genet. 2003 Jun 1;12(11):1329-36. doi: 10.1093/hmg/ddg139. Hum Mol Genet. 2003. PMID: 12761048 - Telomere functions grounding on TERRA firma.
Azzalin CM, Lingner J. Azzalin CM, et al. Trends Cell Biol. 2015 Jan;25(1):29-36. doi: 10.1016/j.tcb.2014.08.007. Epub 2014 Sep 23. Trends Cell Biol. 2015. PMID: 25257515 Review.
Cited by
- Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q.
Lipinski D, Zeyland J, Szalata M, Plawski A, Jarmuz M, Jura J, Korcz A, Smorag Z, Pienkowski M, Slomski R. Lipinski D, et al. J Appl Genet. 2012 Nov;53(4):435-42. doi: 10.1007/s13353-012-0110-4. Epub 2012 Aug 17. J Appl Genet. 2012. PMID: 22898896 Free PMC article. - Telomere elongation involves intra-molecular DNA replication in cells utilizing alternative lengthening of telomeres.
Muntoni A, Neumann AA, Hills M, Reddel RR. Muntoni A, et al. Hum Mol Genet. 2009 Mar 15;18(6):1017-27. doi: 10.1093/hmg/ddn436. Epub 2008 Dec 18. Hum Mol Genet. 2009. PMID: 19095716 Free PMC article. - Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors.
Vega JM, Yu W, Han F, Kato A, Peters EM, Zhang ZJ, Birchler JA. Vega JM, et al. Plant Mol Biol. 2008 Apr;66(6):587-98. doi: 10.1007/s11103-007-9276-2. Epub 2008 Feb 12. Plant Mol Biol. 2008. PMID: 18265944 - Protection from acute cellular injury using Sleeping Beauty mediated telomerase gene transfer.
Song JS, Murase N, Demetris AJ, Michalopoulos GK, Ochoa ER. Song JS, et al. Biochem Biophys Res Commun. 2007 Nov 16;363(2):253-6. doi: 10.1016/j.bbrc.2007.05.213. Epub 2007 Jun 8. Biochem Biophys Res Commun. 2007. PMID: 17869215 Free PMC article. - Telomere position effect and silencing of transgenes near telomeres in the mouse.
Pedram M, Sprung CN, Gao Q, Lo AW, Reynolds GE, Murnane JP. Pedram M, et al. Mol Cell Biol. 2006 Mar;26(5):1865-78. doi: 10.1128/MCB.26.5.1865-1878.2006. Mol Cell Biol. 2006. PMID: 16479005 Free PMC article.
References
- Cell. 1984 Sep;38(2):501-10 - PubMed
- Cell. 1985 Aug;42(1):249-57 - PubMed
- Cell. 1985 Dec;43(2 Pt 1):405-13 - PubMed
- J Biol Chem. 1986 Jan 5;261(1):363-9 - PubMed
- Mol Cell Biol. 1988 Jun;8(6):2523-35 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources