Protease involvement in fodrin cleavage and phosphatidylserine exposure in apoptosis - PubMed (original) (raw)
. 1996 Dec 6;271(49):31075-85.
doi: 10.1074/jbc.271.49.31075.
Affiliations
- PMID: 8940103
- DOI: 10.1074/jbc.271.49.31075
Free article
Protease involvement in fodrin cleavage and phosphatidylserine exposure in apoptosis
D M Vanags et al. J Biol Chem. 1996.
Free article
Abstract
A detailed kinetic analysis of three extranuclear end points of apoptosis, phosphatidylserine exposure, alpha-fodrin degradation, and plasma membrane blebbing, was performed and compared with nuclear fragmentation and the activation of the interleukin-1beta-converting enzyme (ICE)-like proteases in Jurkat T lymphocytes stimulated by anti-Fas monoclonal antibody (anti-Fas mAb) and in monocytic U937 cells stimulated by tumor necrosis factor (TNF) and cycloheximide. Phosphatidylserine exposure was quantitated by plasma clotting time, as well as annexin V-fluorescein isothiocyanate binding, and the ICE-like protease activity was examined by the cleavage of a specific fluorogenic peptide substrate Ac-Asp-Glu-Val-Asp-amino-4-methylcoumarin. VAD-chloromethylketone (VAD-cmk), an inhibitor of ICE-like proteases, effectively inhibited ICE-like activity in both cell types studied, whereas the calpain inhibitor calpeptin was ineffective. VAD-cmk also effectively inhibited all three extranuclear events, as well as nuclear fragmentation, in Jurkat cells stimulated by anti-Fas monoclonal antibody, indicating that ICE-like proteases play an important role in the regulation of this apoptotic system. Calpain inhibitors were ineffective in this system. TNF-induced extranuclear and nuclear changes in U937 cells were inhibited by calpeptin but were not as effectively inhibited by VAD-cmk as in Jurkat cells. This suggests that ICE-like enzymes predominate in anti-Fas monoclonal antibody-stimulated Jurkat cells, whereas proteases affected by calpain inhibitors as well as the ICE-like enzymes are involved in the signaling of apoptotic events in TNF-induced U937 cells. Importantly, the two apoptotic systems seem to be regulated by different proteases.
Similar articles
- Sequential activation of three distinct ICE-like activities in Fas-ligated Jurkat cells.
Greidinger EL, Miller DK, Yamin TT, Casciola-Rosen L, Rosen A. Greidinger EL, et al. FEBS Lett. 1996 Jul 29;390(3):299-303. doi: 10.1016/0014-5793(96)00678-3. FEBS Lett. 1996. PMID: 8706881 - Interleukin 1 beta-converting enzyme related proteases/caspases are involved in TRAIL-induced apoptosis of myeloma and leukemia cells.
Mariani SM, Matiba B, Armandola EA, Krammer PH. Mariani SM, et al. J Cell Biol. 1997 Apr 7;137(1):221-9. doi: 10.1083/jcb.137.1.221. J Cell Biol. 1997. PMID: 9105050 Free PMC article. - Phosphatidylserine externalization during CD95-induced apoptosis of cells and cytoplasts requires ICE/CED-3 protease activity.
Martin SJ, Finucane DM, Amarante-Mendes GP, O'Brien GA, Green DR. Martin SJ, et al. J Biol Chem. 1996 Nov 15;271(46):28753-6. doi: 10.1074/jbc.271.46.28753. J Biol Chem. 1996. PMID: 8910516 - Proteases in apoptosis.
Zhivotovsky B, Burgess DH, Orrenius S. Zhivotovsky B, et al. Experientia. 1996 Oct 31;52(10-11):968-78. doi: 10.1007/BF01920106. Experientia. 1996. PMID: 8917728 Review.
Cited by
- Over Fifty Years of Life, Death, and Cannibalism: A Historical Recollection of Apoptosis and Autophagy.
Izadi M, Ali TA, Pourkarimi E. Izadi M, et al. Int J Mol Sci. 2021 Nov 18;22(22):12466. doi: 10.3390/ijms222212466. Int J Mol Sci. 2021. PMID: 34830349 Free PMC article. Review. - Extracellular Vesicles as an Efficient and Versatile System for Drug Delivery.
Dang XTT, Kavishka JM, Zhang DX, Pirisinu M, Le MTN. Dang XTT, et al. Cells. 2020 Sep 29;9(10):2191. doi: 10.3390/cells9102191. Cells. 2020. PMID: 33003285 Free PMC article. Review. - Fatal dysfunction and disintegration of thrombin-stimulated platelets.
Kim OV, Nevzorova TA, Mordakhanova ER, Ponomareva AA, Andrianova IA, Le Minh G, Daminova AG, Peshkova AD, Alber MS, Vagin O, Litvinov RI, Weisel JW. Kim OV, et al. Haematologica. 2019 Sep;104(9):1866-1878. doi: 10.3324/haematol.2018.202309. Epub 2019 Feb 21. Haematologica. 2019. PMID: 30792211 Free PMC article. - Three cell deaths and a funeral: macrophage clearance of cells undergoing distinct modes of cell death.
Klöditz K, Fadeel B. Klöditz K, et al. Cell Death Discov. 2019 Feb 8;5:65. doi: 10.1038/s41420-019-0146-x. eCollection 2019. Cell Death Discov. 2019. PMID: 30774993 Free PMC article. - Influence of sevoflurane exposure on mitogen-activated protein kinases and Akt/GSK-3β/CRMP-2 signaling pathways in the developing rat brain.
Liu Y, Liu C, Zeng M, Han X, Zhang K, Fu Y, Li J, Li Y. Liu Y, et al. Exp Ther Med. 2018 Feb;15(2):2066-2073. doi: 10.3892/etm.2017.5651. Epub 2017 Dec 18. Exp Ther Med. 2018. PMID: 29434807 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous