A conserved repetitive DNA element located in the centromeres of cereal chromosomes - PubMed (original) (raw)

Comparative Study

A conserved repetitive DNA element located in the centromeres of cereal chromosomes

J Jiang et al. Proc Natl Acad Sci U S A. 1996.

Abstract

Repetitive DNA sequences have been demonstrated to play an important role for centromere function of eukaryotic chromosomes, including those from fission yeast, Drosophila melanogaster, and humans. Here we report on the isolation of a repetitive DNA element located in the centromeric regions of cereal chromosomes. A 745-bp repetitive DNA clone pSau3A9, was isolated from sorghum (Sorghum bicolor). This DNA element is located in the centromeric regions of all sorghum chromosomes, as demonstrated by fluorescence in situ hybridization. Repetitive DNA sequences homologous to pSau3A9 also are present in the centromeric regions of chromosomes from other cereal species, including rice, maize, wheat, barley, rye, and oats. Probe pSau3A9 also hybridized to the centromeric region of B chromosomes from rye and maize. The repetitive nature and its conservation in distantly related plant species indicate that the pSau3A9 family may be associated with centromere function of cereal chromosomes. The absence of DNA sequences homologous to pSau3A9 in dicot species suggests a faster divergence of centromererelated sequences compared with the telomere-related sequences in plants.

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Figures

Figure 1

Figure 1

DNA of sorghum BAC clone 52A4 was digested with restriction enzymes _Dra_I (lane 1), _Sal_I (lane 2), _Sau_3AI (lane 3), _Acc_I (lane 4), and _Hin_dIII (lane 5). The same blot was probed with genomic DNA from wheat, maize, and rice, respectively. A 745_Sau_3AI fragment (arrow) hybridized to the genomic DNA from all the three species. This fragment was subcloned as plasmid pSau3A9.

Figure 2

Figure 2

FISH analysis using pSau3A9 as a probe: 1, sorghum; 2, rice; 3, wheat; 4, barley; 5, rye, 14 A and 2 B (arrows) chromosomes have similar FISH signals at centromeres; 6, maize; 7, oat.

Figure 3

Figure 3

Genomic DNA from sorghum (lane 1), maize (lane 2), wheat (lane 3), rice (lane 4), A. thaliana (lane 5), tobacco (lane 6), tomato (lane 7), and soybean (lane 8) was digested with _Sal_I and probed with pSau3A9. Strong hybridization signals were detected in the four cereal species, and very weak or no signals were detected in the four dicot species.

Figure 4

Figure 4

Nucleotide sequence of pSau3A9.

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