Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells - PubMed (original) (raw)

Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells

B H Spain et al. Mol Cell Biol. 1996 Dec.

Abstract

We have isolated two novel human cDNAs, gps1-1 and gps2, that suppress lethal G-protein subunit-activating mutations in the pheromone response pathway of the yeast Saccharomyces cerevisiae. Suppression of other pathway-activating events was examined. In wild-type cells, expression of either gps1-1 or gps2 led to enhanced recovery from cell cycle arrest induced by pheromone. Sequence analysis indicated that gps1-1 contains only the carboxy-terminal half of the gps1 coding sequence. The predicted gene product of gps1 has striking similarity to the protein encoded by the Arabidopsis FUS6 (COP11) gene, a negative regulator of light-mediated signal transduction that is known to be essential for normal development. A chimeric construct containing gps1 and FUS6 sequences also suppressed the yeast pheromone pathway, indicating functional conservation between these human and plant genes. In addition, when overexpressed in mammalian cells, gps1 or gps2 potently suppressed a RAS- and mitogen-activated protein kinase-mediated signal and interfered with JNK activity, suggesting that signal repression is part of their normal function. For gps1, these results are consistent with the proposed function of FUS6 (COP11) as a signal transduction repressor in plants.

PubMed Disclaimer

Cited by

A protective strategy against hyperinflammatory responses requiring the nontranscriptional actions of GPS2.
Cardamone MD, Krones A, Tanasa B, Taylor H, Ricci L, Ohgi KA, Glass CK, Rosenfeld MG, Perissi V. Cardamone MD, et al. Mol Cell. 2012 Apr 13;46(1):91-104. doi: 10.1016/j.molcel.2012.01.025. Epub 2012 Mar 15. Mol Cell. 2012. PMID: 22424771 Free PMC article.
The Nuclear Receptor-Co-repressor Complex in Control of Liver Metabolism and Disease.
Liang N, Jakobsson T, Fan R, Treuter E. Liang N, et al. Front Endocrinol (Lausanne). 2019 Jun 26;10:411. doi: 10.3389/fendo.2019.00411. eCollection 2019. Front Endocrinol (Lausanne). 2019. PMID: 31293521 Free PMC article. Review.
GPS2/KDM4A pioneering activity regulates promoter-specific recruitment of PPARγ.
Cardamone MD, Tanasa B, Chan M, Cederquist CT, Andricovich J, Rosenfeld MG, Perissi V. Cardamone MD, et al. Cell Rep. 2014 Jul 10;8(1):163-76. doi: 10.1016/j.celrep.2014.05.041. Epub 2014 Jun 19. Cell Rep. 2014. PMID: 24953653 Free PMC article.
Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue.
Cederquist CT, Lentucci C, Martinez-Calejman C, Hayashi V, Orofino J, Guertin D, Fried SK, Lee MJ, Cardamone MD, Perissi V. Cederquist CT, et al. Mol Metab. 2016 Oct 31;6(1):125-137. doi: 10.1016/j.molmet.2016.10.007. eCollection 2017 Jan. Mol Metab. 2016. PMID: 28123943 Free PMC article.
Lineage-coupled clonal capture identifies clonal evolution mechanisms and vulnerabilities of BRAFV600E inhibition resistance in melanoma.
Zhang ZY, Ding Y, Ezhilarasan R, Lhakhang T, Wang Q, Yang J, Modrek AS, Zhang H, Tsirigos A, Futreal A, Draetta GF, Verhaak RGW, Sulman EP. Zhang ZY, et al. Cell Discov. 2022 Oct 6;8(1):102. doi: 10.1038/s41421-022-00462-7. Cell Discov. 2022. PMID: 36202798 Free PMC article.

References

1. Annu Rev Genet. 1993;27:147-79 - PubMed
1. Mol Cell Biol. 1984 Aug;4(8):1440-8 - PubMed
1. EMBO J. 1994 Jan 1;13(1):61-70 - PubMed
1. Mol Cell Biol. 1987 Oct;7(10):3629-36 - PubMed
1. Science. 1994 Aug 26;265(5176):1228-31 - PubMed

Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells - PubMed (original) (raw)