Evidence for apoptosis of human macrophage-like HL-60 cells by Legionella pneumophila infection - PubMed (original) (raw)
Evidence for apoptosis of human macrophage-like HL-60 cells by Legionella pneumophila infection
A Müller et al. Infect Immun. 1996 Dec.
Abstract
Legionella pneumophila, the causative agent of Legionnaires' disease and Pontiac fever, replicates within and eventually kills human macrophages. In this study, we show that L. pneumophila is cytotoxic to HL-60 cells, a macrophage-like cell line. We demonstrate that cell death mediated by L. pneumophila occurred at least in part through apoptosis, as shown by changes in nuclear morphology, an increase in the proportion of fragmented host cell DNA, and the typical ladder pattern of DNA fragmentation indicative of apoptosis. We further sought to determine whether potential virulence factors like the metalloprotease and the macrophage infectivity potentiator of L. pneumophila are involved in the induction of apoptosis. None of these factors are essential for the induction of apoptosis in HL-60 cells but may be involved in other cytotoxic mechanisms that lead to accidental cell death (necrosis). The ability of L. pneumophila to promote cell death may be important for the initiation of infection, bacterial survival, and escape from the host immune response. Alternatively, the triggering of apoptosis in response to bacterial infection may have evolved as a means of the host immune system to reduce or inhibit bacterial replication.
Similar articles
- Induction of rapid cell death by an environmental isolate of Legionella pneumophila in mouse macrophages.
Tao L, Zhu W, Hu BJ, Qu JM, Luo ZQ. Tao L, et al. Infect Immun. 2013 Sep;81(9):3077-88. doi: 10.1128/IAI.00252-13. Epub 2013 Jun 10. Infect Immun. 2013. PMID: 23753633 Free PMC article. - Programmed cell death in Legionella infection.
Speir M, Vince JE, Naderer T. Speir M, et al. Future Microbiol. 2014;9(1):107-18. doi: 10.2217/fmb.13.139. Future Microbiol. 2014. PMID: 24328384 Review. - Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells.
Koriyama T, Yamakuchi M, Takenouchi K, Oyama Y, Takenaka H, Nagakura T, Masamoto I, Hashiguchi T. Koriyama T, et al. Biochem Biophys Res Commun. 2019 Jan 8;508(2):608-613. doi: 10.1016/j.bbrc.2018.11.093. Epub 2018 Dec 1. Biochem Biophys Res Commun. 2019. PMID: 30509489 - Legionella pneumophila induces apoptosis via the mitochondrial death pathway.
Neumeister B, Faigle M, Lauber K, Northoff H, Wesselborg S. Neumeister B, et al. Microbiology (Reading). 2002 Nov;148(Pt 11):3639-3650. doi: 10.1099/00221287-148-11-3639. Microbiology (Reading). 2002. PMID: 12427954 - From amoeba to macrophages: exploring the molecular mechanisms of Legionella pneumophila infection in both hosts.
Escoll P, Rolando M, Gomez-Valero L, Buchrieser C. Escoll P, et al. Curr Top Microbiol Immunol. 2013;376:1-34. doi: 10.1007/82_2013_351. Curr Top Microbiol Immunol. 2013. PMID: 23949285 Review.
Cited by
- Sesquiterpene Alcohol Cedrol Chemosensitizes Human Cancer Cells and Suppresses Cell Proliferation by Destabilizing Plasma Membrane Lipid Rafts.
Mishra SK, Bae YS, Lee YM, Kim JS, Oh SH, Kim HM. Mishra SK, et al. Front Cell Dev Biol. 2021 Jan 21;8:571676. doi: 10.3389/fcell.2020.571676. eCollection 2020. Front Cell Dev Biol. 2021. PMID: 33585438 Free PMC article. - Identification of differentially expressed Legionella genes during its intracellular growth in Acanthamoeba.
Quan FS, Kong HH, Lee HA, Chu KB, Moon EK. Quan FS, et al. Heliyon. 2020 Oct 12;6(10):e05238. doi: 10.1016/j.heliyon.2020.e05238. eCollection 2020 Oct. Heliyon. 2020. PMID: 33088972 Free PMC article. - In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts.
Crépin A, Jégou JF, André S, Ecale F, Croitoru A, Cantereau A, Berjeaud JM, Ladram A, Verdon J. Crépin A, et al. Sci Rep. 2020 Mar 4;10(1):3978. doi: 10.1038/s41598-020-60829-2. Sci Rep. 2020. PMID: 32132569 Free PMC article. - Morphology-based classification of mycobacteria-infected macrophages with convolutional neural network: reveal EsxA-induced morphologic changes indistinguishable by naked eyes.
Bao Y, Zhao X, Wang L, Qian W, Sun J. Bao Y, et al. Transl Res. 2019 Oct;212:1-13. doi: 10.1016/j.trsl.2019.06.001. Epub 2019 Jun 28. Transl Res. 2019. PMID: 31287998 Free PMC article. - Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages.
Jung AL, Stoiber C, Herkt CE, Schulz C, Bertrams W, Schmeck B. Jung AL, et al. PLoS Pathog. 2016 Apr 22;12(4):e1005592. doi: 10.1371/journal.ppat.1005592. eCollection 2016 Apr. PLoS Pathog. 2016. PMID: 27105429 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1978 May;75(5):2458-62 - PubMed
- J Infect Dis. 1990 Jul;162(1):121-6 - PubMed
- Infect Immun. 1990 Aug;58(8):2585-92 - PubMed
- J Immunol. 1990 Sep 15;145(6):1859-67 - PubMed
- Int Rev Exp Pathol. 1991;32:223-54 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Medical