alpha 2-macroglobulin stimulation of protein tyrosine phosphorylation in macrophages via the mannose receptor for Fc gamma receptor-mediated phagocytosis activation - PubMed (original) (raw)

alpha 2-macroglobulin stimulation of protein tyrosine phosphorylation in macrophages via the mannose receptor for Fc gamma receptor-mediated phagocytosis activation

M Murai et al. Immunology. 1996 Nov.

Abstract

Macrophage phagocytic activity has previously been shown to be increased by binding of modified alpha 2-macroglobulin (alpha 2M) with mannose residues at termini of sugar chains to mannose receptors on macrophages, with a subsequent increase in the number of Fc gamma receptors at the cell surface. In the present study, an examination was made of the association of protein tyrosine kinase with the increase in number of Fc gamma receptors following binding of modified alpha 2M to mannose receptors. The phagocytosis of IgG-opsonized sheep red blood cells through the action of the Fc gamma receptor by modified alpha 2M was inhibited by mannose and herbimycin A and slightly so by genistein. The mannose receptor would thus appear to be associated with tyrosine kinase activity. By Western blotting, tyrosine phosphorylated proteins with molecular weights of 32000, 34000, 36000, 65000, 85000 and 110000 appeared or increased upon treating macrophages with modified alpha 2M. The degree of tyrosine phosphorylated proteins was the same for control macrophages following incubation in the presence of mannose and herbimycin A. Genistein treatment affected only tyrosine phosphorylated proteins of 65000 and 110000. The binding of modified alpha 2M to mannose receptors was demonstrated by the inducement of tyrosine kinase activation that was sensitive to herbimycin A, followed by an increase in Fc gamma receptors and consequently greater phagocytosis.

PubMed Disclaimer

Cited by

Phenotypic expression in human monocyte-derived interleukin-4-induced foreign body giant cells and macrophages in vitro: dependence on material surface properties.
McNally AK, Anderson JM. McNally AK, et al. J Biomed Mater Res A. 2015 Apr;103(4):1380-90. doi: 10.1002/jbm.a.35280. Epub 2014 Jul 26. J Biomed Mater Res A. 2015. PMID: 25045023 Free PMC article.
Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
Dalli J, Norling LV, Montero-Melendez T, Federici Canova D, Lashin H, Pavlov AM, Sukhorukov GB, Hinds CJ, Perretti M. Dalli J, et al. EMBO Mol Med. 2014 Jan;6(1):27-42. doi: 10.1002/emmm.201303503. Epub 2013 Dec 15. EMBO Mol Med. 2014. PMID: 24357647 Free PMC article.
Cellular and molecular mechanisms of glial scarring and progressive cavitation: in vivo and in vitro analysis of inflammation-induced secondary injury after CNS trauma.
Fitch MT, Doller C, Combs CK, Landreth GE, Silver J. Fitch MT, et al. J Neurosci. 1999 Oct 1;19(19):8182-98. doi: 10.1523/JNEUROSCI.19-19-08182.1999. J Neurosci. 1999. PMID: 10493720 Free PMC article.
The mannose receptor mediates uptake of pathogenic and nonpathogenic mycobacteria and bypasses bactericidal responses in human macrophages.
Astarie-Dequeker C, N'Diaye EN, Le Cabec V, Rittig MG, Prandi J, Maridonneau-Parini I. Astarie-Dequeker C, et al. Infect Immun. 1999 Feb;67(2):469-77. doi: 10.1128/IAI.67.2.469-477.1999. Infect Immun. 1999. PMID: 9916047 Free PMC article.

References

1. J Immunol. 1994 Feb 15;152(4):1538-45 - PubMed
1. Immunology. 1993 Oct;80(2):253-8 - PubMed
1. J Biol Chem. 1994 Oct 28;269(43):26626-9 - PubMed
1. J Leukoc Biol. 1995 Apr;57(4):687-91 - PubMed
1. Biochem J. 1995 Jul 1;309 ( Pt 1):151-8 - PubMed

alpha 2-macroglobulin stimulation of protein tyrosine phosphorylation in macrophages via the mannose receptor for Fc gamma receptor-mediated phagocytosis activation - PubMed (original) (raw)