[Expression of mRNA, interleukin-1 beta, interleukin 6 and tumor necrosis factor-alpha during regeneration of the sciatic nerve in rats after tissue loss] - PubMed (original) (raw)
[Article in French]
Affiliations
- PMID: 8978144
[Expression of mRNA, interleukin-1 beta, interleukin 6 and tumor necrosis factor-alpha during regeneration of the sciatic nerve in rats after tissue loss]
[Article in French]
C Bizette et al. Chirurgie. 1996.
Abstract
Within the family of proinflammatory cytokines, some exhibit a neurotrophic activity in addition to their proinflammatory functions. However, their role in peripheral nerve regeneration processes is still poorly understood. This study presents the kinetics of expression of mRNAs for different cytokines, IL-1 beta, IL-6, TNF alpha and that of NGF included as control. It was carried out on the rat sciatic nerve regeneration using two models of nerve transection with a 7 mm loss of substance. Nerve regeneration took place through an autologous nerve grafting in the first model, and through a collagenous cylinder in the second one. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to quantify each mRNA of cytokine and that of actin beta gene considered as an endogenous internal standard. The results showed a peak in the expression of IL-1 beta mRNAs in both models at D2 (second bay after operation). This D2-peak was also observed for TNF alpha and IL-6, but only in the model of the cylinder. The level of IL-6 D2-peak was very high compared to the nearly complete absence of IL-6 mRNAs expression in non operated control nerves. According to these results, proinflammatory cytokines and especially IL-6 may play a major role in promoting peripheral nerve regeneration.
Similar articles
- Cytokine gene expression in liver following minor or major hepatectomy in rat.
Scotté M, Masson S, Lyoumi S, Hiron M, Ténière P, Lebreton JP, Daveau M. Scotté M, et al. Cytokine. 1997 Nov;9(11):859-67. doi: 10.1006/cyto.1997.0273. Cytokine. 1997. PMID: 9367547 - Cytokine mRNA profiles in contused spinal cord and axotomized facial nucleus suggest a beneficial role for inflammation and gliosis.
Streit WJ, Semple-Rowland SL, Hurley SD, Miller RC, Popovich PG, Stokes BT. Streit WJ, et al. Exp Neurol. 1998 Jul;152(1):74-87. doi: 10.1006/exnr.1998.6835. Exp Neurol. 1998. PMID: 9682014 - [Studies on the temporal profile of expression and release of major pro-inflammatory cytokines in vital organs following hemorrhagic shock].
Liu YJ, Mao EQ, Li L, Tang YQ, Zhang SD. Liu YJ, et al. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 May;19(5):290-4. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007. PMID: 17490570 Chinese. - Induction of interleukin-6 in dorsal root ganglion neurons after gradual elongation of rat sciatic nerve.
Osamura N, Ikeda K, Ito T, Higashida H, Tomita K, Yokoyama S. Osamura N, et al. Exp Neurol. 2005 Sep;195(1):61-70. doi: 10.1016/j.expneurol.2005.03.019. Exp Neurol. 2005. PMID: 15913608 - Substance P, cytokines, and arthritis.
Kimball ES. Kimball ES. Ann N Y Acad Sci. 1990;594:293-308. doi: 10.1111/j.1749-6632.1990.tb40489.x. Ann N Y Acad Sci. 1990. PMID: 1696078 Review. No abstract available.
Cited by
- Divergent roles for tumor necrosis factor-alpha in the brain.
Sriram K, O'Callaghan JP. Sriram K, et al. J Neuroimmune Pharmacol. 2007 Jun;2(2):140-53. doi: 10.1007/s11481-007-9070-6. Epub 2007 Mar 31. J Neuroimmune Pharmacol. 2007. PMID: 18040839 Review. - Anterograde transport of tumor necrosis factor-alpha in the intact and injured rat sciatic nerve.
Schäfers M, Geis C, Brors D, Yaksh TL, Sommer C. Schäfers M, et al. J Neurosci. 2002 Jan 15;22(2):536-45. doi: 10.1523/JNEUROSCI.22-02-00536.2002. J Neurosci. 2002. PMID: 11784800 Free PMC article. - Sensory impairments and delayed regeneration of sensory axons in interleukin-6-deficient mice.
Zhong J, Dietzel ID, Wahle P, Kopf M, Heumann R. Zhong J, et al. J Neurosci. 1999 Jun 1;19(11):4305-13. doi: 10.1523/JNEUROSCI.19-11-04305.1999. J Neurosci. 1999. PMID: 10341234 Free PMC article. - Interleukin-1β influences functional regeneration following nerve injury in mice through nuclear factor-κB signaling pathway.
Wu R, Chen B, Jia X, Qiu Y, Liu M, Huang C, Feng J, Wu Q. Wu R, et al. Immunology. 2019 Mar;156(3):235-248. doi: 10.1111/imm.13022. Epub 2019 Jan 6. Immunology. 2019. PMID: 30418673 Free PMC article. - Differential acute and chronic responses of tumor necrosis factor-deficient mice to experimental brain injury.
Scherbel U, Raghupathi R, Nakamura M, Saatman KE, Trojanowski JQ, Neugebauer E, Marino MW, McIntosh TK. Scherbel U, et al. Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8721-6. doi: 10.1073/pnas.96.15.8721. Proc Natl Acad Sci U S A. 1999. PMID: 10411942 Free PMC article.