RSC, an essential, abundant chromatin-remodeling complex - PubMed (original) (raw)

RSC, an essential, abundant chromatin-remodeling complex

B R Cairns et al. Cell. 1996.

Free article

Abstract

A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWII/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.

PubMed Disclaimer

Similar articles

• The core histone N-terminal domains are required for multiple rounds of catalytic chromatin remodeling by the SWI/SNF and RSC complexes.
  Logie C, Tse C, Hansen JC, Peterson CL. Logie C, et al. Biochemistry. 1999 Feb 23;38(8):2514-22. doi: 10.1021/bi982109d. Biochemistry. 1999. PMID: 10029546
• The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling.
  Sengupta SM, VanKanegan M, Persinger J, Logie C, Cairns BR, Peterson CL, Bartholomew B. Sengupta SM, et al. J Biol Chem. 2001 Apr 20;276(16):12636-44. doi: 10.1074/jbc.m010470200. J Biol Chem. 2001. PMID: 11304548
• Sth1p, a Saccharomyces cerevisiae Snf2p/Swi2p homolog, is an essential ATPase in RSC and differs from Snf/Swi in its interactions with histones and chromatin-associated proteins.
  Du J, Nasir I, Benton BK, Kladde MP, Laurent BC. Du J, et al. Genetics. 1998 Nov;150(3):987-1005. doi: 10.1093/genetics/150.3.987. Genetics. 1998. PMID: 9799253 Free PMC article.
• Remodeling chromatin structures for transcription: what happens to the histones?
  Steger DJ, Workman JL. Steger DJ, et al. Bioessays. 1996 Nov;18(11):875-84. doi: 10.1002/bies.950181106. Bioessays. 1996. PMID: 8939065 Review.
• The mammalian SWI/SNF complex and the control of cell growth.
  Muchardt C, Yaniv M. Muchardt C, et al. Semin Cell Dev Biol. 1999 Apr;10(2):189-95. doi: 10.1006/scdb.1999.0300. Semin Cell Dev Biol. 1999. PMID: 10441072 Review.

Cited by

• ATP-dependent remodeling of chromatin condensates uncovers distinct mesoscale effects of two remodelers.
  Moore C, Wong E, Kaur U, Chio US, Zhou Z, Ostrowski M, Wu K, Irkliyenko I, Wang S, Ramani V, Narlikar GJ. Moore C, et al. bioRxiv [Preprint]. 2024 Sep 10:2024.09.10.611504. doi: 10.1101/2024.09.10.611504. bioRxiv. 2024. PMID: 39314305 Free PMC article. Preprint.
• Transcriptional activation domains interact with ATPase subunits of yeast chromatin remodelling complexes SWI/SNF, RSC and INO80.
  Wendegatz EC, Engelhardt M, Schüller HJ. Wendegatz EC, et al. Curr Genet. 2024 Sep 5;70(1):15. doi: 10.1007/s00294-024-01300-x. Curr Genet. 2024. PMID: 39235627 Free PMC article.
• A genome-wide comprehensive analysis of nucleosome positioning in yeast.
  Zeitler L, André K, Alberti A, Denby Wilkes C, Soutourina J, Goldar A. Zeitler L, et al. PLoS Comput Biol. 2024 Jan 24;20(1):e1011799. doi: 10.1371/journal.pcbi.1011799. eCollection 2024 Jan. PLoS Comput Biol. 2024. PMID: 38266035 Free PMC article.
• Context-specific functions of chromatin remodellers in development and disease.
  Gourisankar S, Krokhotin A, Wenderski W, Crabtree GR. Gourisankar S, et al. Nat Rev Genet. 2024 May;25(5):340-361. doi: 10.1038/s41576-023-00666-x. Epub 2023 Nov 24. Nat Rev Genet. 2024. PMID: 38001317 Review.
• A SAM-key domain required for enzymatic activity of the Fun30 nucleosome remodeler.
  Karl LA, Galanti L, Bantele SC, Metzner F, Šafarić B, Rajappa L, Foster B, Pires VB, Bansal P, Chacin E, Basquin J, Duderstadt KE, Kurat CF, Bartke T, Hopfner KP, Pfander B. Karl LA, et al. Life Sci Alliance. 2023 Jul 19;6(9):e202201790. doi: 10.26508/lsa.202201790. Print 2023 Sep. Life Sci Alliance. 2023. PMID: 37468166 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database