RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants - PubMed (original) (raw)

Review

. 1996 Oct;32(1-2):79-88.

doi: 10.1007/BF00039378.

Affiliations

• PMID: 8980475
• DOI: 10.1007/BF00039378

Review

RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants

D C Baulcombe. Plant Mol Biol. 1996 Oct.

Abstract

Post-transcriptional gene silencing in transgenic plants is the manifestation of a mechanism that suppresses RNA accumulation in a sequence-specific manner. The target RNA species may be the products of transgenes, endogenous plant genes or viral RNAs. For an RNA to be a target it is necessary only that it has sequence homology to the sense RNA product of the transgene. There are three current hypotheses to account for the mechanism of post transcriptional gene silencing. These models all require production of an antisense RNA of the RNA targets to account for the specificity of the mechanism. There could be either direct transcription of the antisense RNA from the transgene, antisense RNA produced in response to over expression of the transgene or antisense RNA produced in response to the production of an aberrant sense RNA product of the transgene. To determine which of these models is correct it will be necessary to find out whether transgene methylation, which is frequently associated with the potential of transgenes to confer post-transcriptional gene silencing, is a cause or a consequence of the process.

PubMed Disclaimer

Similar articles

• HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal.
  Mallory AC, Ely L, Smith TH, Marathe R, Anandalakshmi R, Fagard M, Vaucheret H, Pruss G, Bowman L, Vance VB. Mallory AC, et al. Plant Cell. 2001 Mar;13(3):571-83. doi: 10.1105/tpc.13.3.571. Plant Cell. 2001. PMID: 11251097 Free PMC article.
• Relationship between small antisense RNAs and aberrant RNAs associated with sense transgene mediated gene silencing in tomato.
  Han Y, Grierson D. Han Y, et al. Plant J. 2002 Feb;29(4):509-19. doi: 10.1046/j.1365-313x.2002.01236.x. Plant J. 2002. PMID: 11846883
• Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants.
  Pang SZ, Jan FJ, Gonsalves D. Pang SZ, et al. Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8261-6. doi: 10.1073/pnas.94.15.8261. Proc Natl Acad Sci U S A. 1997. PMID: 9223349 Free PMC article.
• RNA degradation and models for post-transcriptional gene-silencing.
  Meins F Jr. Meins F Jr. Plant Mol Biol. 2000 Jun;43(2-3):261-73. doi: 10.1023/a:1006443731515. Plant Mol Biol. 2000. PMID: 10999409 Review.
• RNA silencing and antiviral defense in plants.
  Wang MB, Metzlaff M. Wang MB, et al. Curr Opin Plant Biol. 2005 Apr;8(2):216-22. doi: 10.1016/j.pbi.2005.01.006. Curr Opin Plant Biol. 2005. PMID: 15753004 Review.

Cited by

• RNA therapeutics: RNAi and antisense mechanisms and clinical applications.
  Chery J. Chery J. Postdoc J. 2016 Jul;4(7):35-50. doi: 10.14304/surya.jpr.v4n7.5. Postdoc J. 2016. PMID: 27570789 Free PMC article.
• Transcriptionally silenced transgenes in maize are activated by three mutations defective in paramutation.
  McGinnis KM, Springer C, Lin Y, Carey CC, Chandler V. McGinnis KM, et al. Genetics. 2006 Jul;173(3):1637-47. doi: 10.1534/genetics.106.058669. Epub 2006 May 15. Genetics. 2006. PMID: 16702420 Free PMC article.
• Inhibition of viruses by RNA interference.
  Stram Y, Kuzntzova L. Stram Y, et al. Virus Genes. 2006 Jun;32(3):299-306. doi: 10.1007/s11262-005-6914-0. Virus Genes. 2006. PMID: 16732482 Free PMC article. Review.
• Cloning of the Arabidopsis RSF1 gene by using a mapping strategy based on high-density DNA arrays and denaturing high-performance liquid chromatography.
  Spiegelman JI, Mindrinos MN, Fankhauser C, Richards D, Lutes J, Chory J, Oefner PJ. Spiegelman JI, et al. Plant Cell. 2000 Dec;12(12):2485-2498. doi: 10.1105/tpc.12.12.2485. Plant Cell. 2000. PMID: 11148292 Free PMC article.
• Recent Advances in Plant Gene Silencing Methods.
  Pandey P, Mysore KS, Senthil-Kumar M. Pandey P, et al. Methods Mol Biol. 2022;2408:1-22. doi: 10.1007/978-1-0716-1875-2_1. Methods Mol Biol. 2022. PMID: 35325413

References

1. 1. Mol Plant Microbe Interact. 1995 Sep-Oct;8(5):674-82 - PubMed
2. 1. Mol Gen Genet. 1994 May 25;243(4):390-9 - PubMed
3. 1. Plant Cell. 1996 Feb;8(2):179-188 - PubMed
4. 1. Plant Mol Biol. 1990 Dec;15(6):851-64 - PubMed
5. 1. Plant J. 1994 Jan;5(1):33-44 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database