Discrete cross-linking products identified during membrane protein biosynthesis - PubMed (original) (raw)
. 1997 Jan 17;272(3):1983-9.
doi: 10.1074/jbc.272.3.1983.
Affiliations
- PMID: 8999890
- DOI: 10.1074/jbc.272.3.1983
Free article
Discrete cross-linking products identified during membrane protein biosynthesis
V Laird et al. J Biol Chem. 1997.
Free article
Abstract
We have investigated the molecular details of the membrane insertion of the multiple-spanning membrane protein opsin. Using heterobifunctional cross-linking reagents the endoplasmic reticulum (ER) proteins adjacent to a series of defined translocation intermediates were determined. Once the nascent opsin chain reaches a critical minimum length Sec61alpha is the major ER component adjacent to the polypeptide. Using a homobifunctional reagent, the cross-linking partners from a single cysteine residue in the nascent chain were analyzed. This approach identified chain length-dependent cross-linking products between nascent opsin and a 21-kDa ribosomal protein, followed by Sec61beta and finally with Sec61alpha. Our data support a model where the sequential transmembrane domains of a multiple-spanning membrane protein are integrated at an ER insertion site similar to that mediating the insertion of single-spanning membrane proteins.
Similar articles
- Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation complex.
Knight BC, High S. Knight BC, et al. Biochem J. 1998 Apr 1;331 ( Pt 1)(Pt 1):161-7. doi: 10.1042/bj3310161. Biochem J. 1998. PMID: 9512475 Free PMC article. - Different transmembrane domains associate with distinct endoplasmic reticulum components during membrane integration of a polytopic protein.
Meacock SL, Lecomte FJ, Crawshaw SG, High S. Meacock SL, et al. Mol Biol Cell. 2002 Dec;13(12):4114-29. doi: 10.1091/mbc.e02-04-0198. Mol Biol Cell. 2002. PMID: 12475939 Free PMC article. - Specific transmembrane segments are selectively delayed at the ER translocon during opsin biogenesis.
Ismail N, Crawshaw SG, Cross BC, Haagsma AC, High S. Ismail N, et al. Biochem J. 2008 May 1;411(3):495-506. doi: 10.1042/BJ20071597. Biochem J. 2008. PMID: 18248332 - A second signal recognition event required for translocation into the endoplasmic reticulum.
Siegel V. Siegel V. Cell. 1995 Jul 28;82(2):167-70. doi: 10.1016/0092-8674(95)90301-1. Cell. 1995. PMID: 7628005 Review. - Protein insertion into the membrane of the endoplasmic reticulum: the architecture of the translocation site.
Martoglio B, Dobberstein B. Martoglio B, et al. Cold Spring Harb Symp Quant Biol. 1995;60:41-5. doi: 10.1101/sqb.1995.060.01.007. Cold Spring Harb Symp Quant Biol. 1995. PMID: 8824376 Review. No abstract available.
Cited by
- The ER Protein Translocation Channel Subunit Sbh1 Controls Virulence of Cryptococcus neoformans.
Santiago-Tirado FH, Hurtaux T, Geddes-McAlister J, Nguyen D, Helms V, Doering TL, Römisch K. Santiago-Tirado FH, et al. mBio. 2023 Feb 28;14(1):e0338422. doi: 10.1128/mbio.03384-22. Epub 2023 Feb 7. mBio. 2023. PMID: 36749043 Free PMC article. - Cancer associated mutations in Sec61γ alter the permeability of the ER translocase.
Witham CM, Paxman AL, Baklous L, Steuart RFL, Schulz BL, Mousley CJ. Witham CM, et al. PLoS Genet. 2021 Aug 30;17(8):e1009780. doi: 10.1371/journal.pgen.1009780. eCollection 2021 Aug. PLoS Genet. 2021. PMID: 34460824 Free PMC article. - Mechanistic insights into the inhibition of Sec61-dependent co- and post-translational translocation by mycolactone.
McKenna M, Simmonds RE, High S. McKenna M, et al. J Cell Sci. 2016 Apr 1;129(7):1404-15. doi: 10.1242/jcs.182352. Epub 2016 Feb 11. J Cell Sci. 2016. PMID: 26869228 Free PMC article. - Reorientation of the first signal-anchor sequence during potassium channel biogenesis at the Sec61 complex.
Watson HR, Wunderley L, Andreou T, Warwicker J, High S. Watson HR, et al. Biochem J. 2013 Dec 1;456(2):297-309. doi: 10.1042/BJ20130100. Biochem J. 2013. PMID: 24015703 Free PMC article. - N-acetylation and phosphorylation of Sec complex subunits in the ER membrane.
Soromani C, Zeng N, Hollemeyer K, Heinzle E, Klein MC, Tretter T, Seaman MN, Römisch K. Soromani C, et al. BMC Cell Biol. 2012 Dec 13;13:34. doi: 10.1186/1471-2121-13-34. BMC Cell Biol. 2012. PMID: 23237413 Free PMC article.